##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921282_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	779845
Sequences flagged as poor quality	0
Sequence length	125
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.104355352666236	31.0	31.0	34.0	26.0	34.0
2	30.302279299091484	31.0	31.0	34.0	26.0	34.0
3	30.40153363809475	33.0	31.0	34.0	26.0	34.0
4	33.83392981938719	37.0	35.0	37.0	30.0	37.0
5	33.78057306259577	37.0	35.0	37.0	30.0	37.0
6	33.92140104764408	37.0	35.0	37.0	30.0	37.0
7	33.836972731760795	37.0	35.0	37.0	29.0	37.0
8	33.86933685540075	37.0	35.0	37.0	30.0	37.0
9	35.44138514704845	39.0	37.0	39.0	30.0	39.0
10-11	35.44084144926235	39.0	37.0	39.0	30.0	39.0
12-13	35.35332341683283	39.0	37.0	39.0	29.0	39.0
14-15	36.65727612538389	40.0	38.0	41.0	28.5	41.0
16-17	36.64118061922562	40.0	38.0	41.0	29.0	41.0
18-19	36.6264122998801	40.0	38.0	41.0	28.5	41.0
20-21	36.66801351550629	40.0	38.0	41.0	29.0	41.0
22-23	36.62260449191827	40.0	38.0	41.0	28.5	41.0
24-25	36.627181042386624	40.0	38.0	41.0	29.0	41.0
26-27	36.604768896383256	40.0	38.0	41.0	29.0	41.0
28-29	36.547579326661065	40.0	38.0	41.0	29.0	41.0
30-31	36.47059095076585	40.0	38.0	41.0	29.0	41.0
32-33	36.388445139739304	40.0	37.0	41.0	29.0	41.0
34-35	36.27739037885733	40.0	37.0	41.0	28.0	41.0
36-37	36.15479293962262	40.0	37.0	41.0	27.0	41.0
38-39	36.048733402150425	40.0	37.0	41.0	27.0	41.0
40-41	35.94394463002263	40.0	36.5	41.0	27.0	41.0
42-43	35.74395104155313	40.0	36.0	41.0	26.0	41.0
44-45	35.47936769486244	40.0	35.0	41.0	25.5	41.0
46-47	35.252809853240066	40.0	35.0	41.0	24.5	41.0
48-49	35.03799537087498	39.5	35.0	41.0	24.0	41.0
50-51	34.856559957427436	39.0	35.0	41.0	24.0	41.0
52-53	34.655438580743606	39.0	35.0	41.0	23.0	41.0
54-55	34.44395232385923	39.0	34.5	40.5	23.0	41.0
56-57	34.19484384717476	38.0	34.0	40.0	22.0	41.0
58-59	33.926821996678825	38.0	34.0	40.0	21.5	41.0
60-61	33.634010604671445	37.5	34.0	40.0	20.0	41.0
62-63	33.30482467669857	37.0	33.0	40.0	18.5	41.0
64-65	32.962527810013526	36.0	33.0	39.0	17.5	41.0
66-67	32.62973154921811	36.0	33.0	39.0	16.5	41.0
68-69	32.27996268489251	35.0	32.5	39.0	13.5	40.0
70-71	32.2440965832954	35.0	33.0	38.5	13.5	40.0
72-73	31.963184350736363	35.0	33.0	37.0	12.0	39.5
74-75	31.64450692124717	35.0	33.0	37.0	9.0	39.0
76-77	31.347327994665605	35.0	32.0	36.5	7.0	39.0
78-79	31.06379216382743	35.0	32.0	36.0	6.5	38.0
80-81	30.784919439119314	35.0	32.0	35.5	2.0	37.0
82-83	30.56427559322686	35.0	32.0	35.0	2.0	37.0
84-85	30.345725753194543	35.0	32.0	35.0	2.0	36.0
86-87	30.169836954779477	35.0	32.0	35.0	2.0	36.0
88-89	30.029114118831306	35.0	31.0	35.0	2.0	36.0
90-91	29.904336759227796	35.0	31.0	35.0	2.0	35.5
92-93	29.78597093012073	35.0	31.0	35.0	2.0	35.0
94-95	29.667471100026287	35.0	31.0	35.0	2.0	35.0
96-97	29.581505299129958	35.0	31.0	35.0	2.0	35.0
98-99	29.491377132635332	35.0	31.0	35.0	2.0	35.0
100-101	29.396184498201563	34.0	31.0	35.0	2.0	35.0
102-103	28.546550276016387	33.5	29.5	34.5	2.0	35.0
104-105	29.152531592816523	34.0	30.5	35.0	2.0	35.0
106-107	29.23446518218364	34.0	31.0	35.0	2.0	35.0
108-109	29.178654091518187	34.0	31.0	35.0	2.0	35.0
110-111	29.100477659021983	34.0	30.5	35.0	2.0	35.0
112-113	29.006828279978713	34.0	30.0	35.0	2.0	35.0
114-115	28.887037808795338	34.0	30.0	35.0	2.0	35.0
116-117	28.76255089152332	34.0	30.0	35.0	2.0	35.0
118-119	28.76400759125211	34.0	30.0	35.0	2.0	35.0
120-121	28.94243022651937	35.0	31.0	35.0	2.0	35.0
122-123	28.812581987446222	35.0	31.0	35.0	2.0	35.0
124-125	28.22607569452904	34.5	30.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	30788.0
3	4427.0
4	2764.0
5	2162.0
6	2367.0
7	2649.0
8	2860.0
9	2916.0
10	3355.0
11	3573.0
12	3710.0
13	3609.0
14	3675.0
15	3397.0
16	2747.0
17	2518.0
18	2389.0
19	2209.0
20	2128.0
21	2298.0
22	2519.0
23	3295.0
24	4767.0
25	6117.0
26	6344.0
27	6999.0
28	8254.0
29	10019.0
30	12310.0
31	15364.0
32	20061.0
33	27425.0
34	43172.0
35	71585.0
36	148660.0
37	239316.0
38	67032.0
39	65.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.274891241894444	16.765036321103178	10.674454157432487	25.285618279569892
2	17.124587691534273	18.91617377743807	37.68242886236207	26.276809668665585
3	21.4304035129118	22.36877879120541	27.578640388226	28.62217730765679
4	13.457673766032569	13.933729276772672	34.50825338157158	38.100343575623185
5	13.860116417898963	38.35224686032032	32.94736828606128	14.840268435719443
6	34.91756049881863	33.67718399901488	16.54639162033961	14.858863881826881
7	30.870429507650083	31.88167699075321	18.41175791620176	18.836135585394945
8	28.63762339159687	35.00557242030285	18.058361003061304	18.29844318503898
9	27.827934760584476	15.71981112476179	17.439796659031977	39.01245745562176
10-11	27.310179705223415	25.518577747227734	25.706772577288124	21.464469970260726
12-13	26.395413106399566	22.54577211469286	26.31866137804298	24.740153400864596
14-15	23.6549315010522	24.09677952995218	23.359532593906334	28.888756375089287
16-17	24.56837104028879	27.41138037272019	24.055359816364934	23.964888770626082
18-19	24.36614588743423	26.69150659213407	25.5579365293097	23.384410991122003
20-21	24.908822541222214	25.57277653956537	25.694731199618364	23.823669719594047
22-23	24.940465427629427	25.11762646528144	25.532989911502835	24.4089181955863
24-25	24.47646698247495	25.247115278572142	25.727752571819885	24.548665167133027
26-27	24.153617990150096	25.571729837414374	25.6783591271915	24.596293045244032
28-29	24.254950804535795	26.184169608135445	25.425575229466624	24.135304357862136
30-31	24.780135368390273	25.657926796426768	25.548667731899798	24.01327010328316
32-33	24.312177793736694	25.579714791351403	25.607927877093537	24.500179537818358
34-35	24.4418468506883	25.73797916467626	25.7261809878793	24.093992996756143
36-37	24.489509341629596	25.796721933753876	25.691308802765878	24.022459921850654
38-39	24.536958074556065	25.986837465006555	25.568198576794494	23.908005883642883
40-41	24.53422999189519	25.649346485693474	25.755978578683326	24.060444943728008
42-43	24.2872559184545	25.686807461795585	25.5182354634704	24.507701156279516
44-45	24.06884709394629	25.88774356041273	25.810286411913015	24.233122933727973
46-47	24.02865214865466	25.603866283133296	25.69382905406359	24.673652514148454
48-49	24.08636031083146	25.862567559162557	25.62782171113757	24.42325041886841
50-51	24.18828233501796	25.9448933504489	25.623399423183212	24.243424891349928
52-53	24.27628879199795	25.917542959733264	25.61740189792254	24.188766350346242
54-55	23.84817262518859	25.640890825583952	26.049652888873787	24.46128366035367
56-57	24.184035815092148	25.785314632404145	25.56294507574543	24.46770447675828
58-59	24.07434836497332	25.957358950535948	25.806035867417897	24.162256817072837
60-61	24.464848471247528	25.94612097293866	25.779027381933243	23.810003173880563
62-63	24.353630269396863	25.994825579717812	25.62652483497318	24.025019315912143
64-65	24.21367618366139	25.577203838600283	25.805719429788198	24.403400547950124
66-67	24.178871547079996	25.81805078410025	25.640123055458307	24.362954613361442
68-69	23.9800177739477	25.79909027139254	25.818839102171985	24.402052852487778
70-71	24.022751856394777	25.609367476078944	25.949003884948084	24.4188767825782
72-73	24.052472058737724	26.23711938425671	25.477635128246725	24.23277342875884
74-75	23.92473807722394	26.060065913491748	25.68112745412344	24.334068555160872
76-77	23.92689449727464	25.681629177569505	25.773639418597867	24.61783690655799
78-79	23.845658660942245	25.708403381819068	25.894665030385333	24.55127292685335
80-81	24.030507235690514	25.916916191660842	25.611523245898066	24.441053326750577
82-83	24.226019492177482	26.0639907668633	25.57078738137984	24.13920235957938
84-85	24.20359171623813	26.145583325264678	25.401001453640287	24.249823504856902
86-87	23.825578234084137	26.282399831749142	25.589198602697145	24.30282333146958
88-89	23.76361480808947	25.87344119383922	25.683393316393165	24.67955068167814
90-91	24.143162257526125	25.646174887038413	25.841673607544497	24.36898924789097
92-93	24.103878416521646	25.88137377737481	25.66535047840601	24.349397327697535
94-95	24.01753808885358	25.824502413154338	25.702418027452318	24.455541470539767
96-97	23.777264519703294	25.670584071159624	25.87442675257256	24.677724656564518
98-99	23.73520272791742	25.759340026596938	25.68028017835594	24.825177067129697
100-101	23.774168768763378	25.942288628672046	25.78320468544212	24.500337917122458
102-103	23.60342395486022	25.873749679404977	25.87015901513208	24.65266735060272
104-105	23.71719285691844	25.89851161222026	25.74590906555305	24.638386465308248
106-107	23.698696628752394	25.80126201372044	25.874487278873488	24.625554078653682
108-109	23.748603788973686	25.790951141911467	25.780948436051293	24.67949663306356
110-111	23.62431014329014	26.15424961992558	25.764781544573705	24.456658692210574
112-113	23.569105174977445	26.19426378926405	25.755946552996566	24.480684482761937
114-115	23.594829868989923	26.199386914123735	25.77801059339557	24.427772623490768
116-117	23.45856562593171	26.177214540581613	25.73358510164237	24.63063473184431
118-119	23.502837701943047	25.934566536051467	25.86155302373549	24.701042738269997
120-121	23.428542848129023	26.08912554472308	25.90329770671263	24.579033900435267
122-123	23.4524151479557	26.18462019830014	25.741637899639407	24.621326754104754
124-125	23.370670530756705	26.067412128284367	25.7418924637421	24.820024877216827
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	157.0
1	140.5
2	116.0
3	101.0
4	82.0
5	73.0
6	90.0
7	110.5
8	118.5
9	144.0
10	155.5
11	157.0
12	178.0
13	190.0
14	227.5
15	258.0
16	286.5
17	331.5
18	356.0
19	392.0
20	463.5
21	520.5
22	581.5
23	754.0
24	938.5
25	1202.0
26	1633.0
27	2073.5
28	2542.0
29	3136.0
30	3957.0
31	4990.5
32	6411.5
33	7988.5
34	9352.5
35	10781.0
36	12506.0
37	14044.0
38	15073.0
39	16340.5
40	17558.5
41	18404.0
42	19544.5
43	21541.0
44	23732.5
45	25682.0
46	27394.5
47	28995.0
48	31195.0
49	33478.5
50	34405.0
51	34608.0
52	34667.0
53	34426.5
54	34035.5
55	33125.5
56	31350.0
57	28741.0
58	26058.5
59	23254.0
60	20317.0
61	17518.0
62	14663.5
63	11377.0
64	8424.5
65	6319.5
66	4744.0
67	3575.5
68	2737.5
69	2100.5
70	1625.0
71	1309.0
72	1018.5
73	809.0
74	646.0
75	494.0
76	370.0
77	238.0
78	151.5
79	103.5
80	70.0
81	47.5
82	28.0
83	17.0
84	15.0
85	9.0
86	7.5
87	7.0
88	4.0
89	5.0
90	6.5
91	4.0
92	1.0
93	2.0
94	1.5
95	1.5
96	1.5
97	0.0
98	0.0
99	0.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.017054671120543187
2	0.011412524283671755
3	0.011925446723387339
4	0.013335983432605197
5	0.008719681475164937
6	0.03116003821272176
7	0.014746520141823054
8	0.014361828312036367
9	0.009104373304951625
10-11	0.008719681475164937
12-13	0.007437375375875976
14-15	0.006667991716302599
16-17	0.004808647872333605
18-19	0.006667991716302599
20-21	0.006026838666658118
22-23	0.005898608056729222
24-25	0.006026838666658118
26-27	0.005193339702120293
28-29	0.006090953971622566
30-31	0.006026838666658118
32-33	0.008334989645378248
34-35	0.008142643730484905
36-37	0.007180914156018184
38-39	0.007180914156018184
40-41	0.007822067205662664
42-43	0.007629721290769319
44-45	0.007565605985804871
46-47	0.010001987574453898
48-49	0.008399104950342696
50-51	0.006155069276587015
52-53	0.005770377446800326
54-55	0.00660387641133815
56-57	0.00795029781559156
58-59	0.007245029460982631
60-61	0.005449800921978085
62-63	0.006090953971622566
64-65	0.0037828029929024357
66-67	0.0043598407375824684
68-69	0.006667991716302599
70-71	0.005193339702120293
72-73	0.005065109092191398
74-75	0.004488071347511364
76-77	0.005193339702120293
78-79	0.003590457078009092
80-81	0.003846918297866884
82-83	0.005770377446800326
84-85	0.010066102879418346
86-87	0.007309144765947079
88-89	0.00532157031204919
90-91	0.005706262141835877
92-93	0.008719681475164937
94-95	0.007245029460982631
96-97	0.009873756964525002
98-99	0.007180914156018184
100-101	0.008719681475164937
102-103	0.005770377446800326
104-105	0.00532157031204919
106-107	0.007629721290769319
108-109	0.007180914156018184
110-111	0.008142643730484905
112-113	0.005706262141835877
114-115	0.0025004968936134745
116-117	0.0035263417730446436
118-119	0.15669780533311106
120-121	0.011668985503529548
122-123	0.0021799203687912342
124-125	0.001923459148933442
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	779845.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	70.98773783591798
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.5733042018311	60.03667546596928
2	9.237022147834878	13.114306132301406
3	2.569111001770794	5.471261347952333
4	1.1345615043987936	3.221598185319451
5	0.6290530333901634	2.2327525909594943
6	0.42096147528434774	1.792986170790393
7	0.2867663985126589	1.4249828542436909
8	0.20583244181766247	1.1689263534303254
9	0.16812208324387704	1.0741145732770252
>10	0.7481755347683795	8.896809871680635
>50	0.02221440240839901	1.02615168724139
>100	0.004875774738951565	0.539434766834631
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	1.2823060992889614E-4
3	0.0	0.0	0.0	0.0	1.2823060992889614E-4
4	0.0	0.0	0.0	0.0	2.564612198577923E-4
5	0.0	0.0	0.0	0.0	2.564612198577923E-4
6	0.0	0.0	0.0	0.0	3.8469182978668836E-4
7	0.0	0.0	0.0	0.0	3.8469182978668836E-4
8	0.0	0.0	0.0	0.0	3.8469182978668836E-4
9	0.0	0.0	0.0	0.0	3.8469182978668836E-4
10-11	0.0	0.0	0.0	0.0	3.8469182978668836E-4
12-13	0.0	0.0	0.0	0.0	3.8469182978668836E-4
14-15	6.411530496444807E-5	0.0	0.0	0.0	5.770377446800326E-4
16-17	1.2823060992889614E-4	0.0	0.0	0.0	7.693836595733767E-4
18-19	1.2823060992889614E-4	0.0	0.0	0.0	7.693836595733767E-4
20-21	1.2823060992889614E-4	0.0	0.0	0.0	7.693836595733767E-4
22-23	1.2823060992889614E-4	0.0	0.0	0.0	8.976142695022728E-4
24-25	1.2823060992889614E-4	0.0	0.0	1.2823060992889614E-4	0.001025844879431169
26-27	1.2823060992889614E-4	0.0	0.0	7.052683546089287E-4	0.0012823060992889614
28-29	1.2823060992889614E-4	0.0	0.0	0.002885188723400163	0.0012823060992889614
30-31	1.2823060992889614E-4	0.0	0.0	0.009681411049631657	0.0012823060992889614
32-33	1.2823060992889614E-4	0.0	0.0	0.018978130269476628	0.0012823060992889614
34-35	1.2823060992889614E-4	0.0	0.0	0.029557155588610558	0.0015387673191467534
36-37	1.2823060992889614E-4	0.0	0.0	0.04558598182972257	0.0015387673191467534
38-39	1.923459148933442E-4	0.0	0.0	0.0809776301700979	0.0016669979290756497
40-41	2.564612198577923E-4	0.0	0.0	0.13143637517711854	0.0016669979290756497
42-43	2.564612198577923E-4	0.0	0.0	0.173431899928832	0.0017311132340400977
44-45	3.205765248222403E-4	0.0	0.0	0.21946668889330573	0.001859343843968994
46-47	3.8469182978668836E-4	0.0	0.0	0.27063070225493524	0.001923459148933442
48-49	3.8469182978668836E-4	0.0	0.0	0.3253851726945739	0.002051689758862338
50-51	3.8469182978668836E-4	0.0	0.0	0.3900134000987376	0.002051689758862338
52-53	3.8469182978668836E-4	0.0	0.0	0.4570780090915503	0.0021799203687912342
54-55	3.8469182978668836E-4	6.411530496444807E-5	0.0	0.5241426180843629	0.0023081509787201302
56-57	3.8469182978668836E-4	1.2823060992889614E-4	0.0	0.6071719380133231	0.0023081509787201302
58-59	3.8469182978668836E-4	1.2823060992889614E-4	0.0	0.696163981303977	0.0023081509787201302
60-61	5.129224397155845E-4	1.2823060992889614E-4	0.0	0.796889125403125	0.0023081509787201302
62-63	5.129224397155845E-4	1.2823060992889614E-4	0.0	0.9107579070199847	0.0025646121985779227
64-65	5.129224397155845E-4	1.2823060992889614E-4	0.0	1.0105854368496303	0.0025646121985779227
66-67	5.129224397155845E-4	1.2823060992889614E-4	0.0	1.1276599837147125	0.0025646121985779227
68-69	5.129224397155845E-4	1.2823060992889614E-4	0.0	1.2571087844379332	0.0025646121985779227
70-71	5.129224397155845E-4	1.923459148933442E-4	0.0	1.4072668286646706	0.0025646121985779227
72-73	6.411530496444807E-4	2.564612198577923E-4	0.0	1.5507568811751054	0.0032698805531868515
74-75	6.411530496444807E-4	2.564612198577923E-4	0.0	1.7010431560117716	0.0033339958581512993
76-77	6.411530496444807E-4	2.564612198577923E-4	0.0	1.8787707813732215	0.0033339958581512993
78-79	6.411530496444807E-4	2.564612198577923E-4	0.0	2.0822086440254153	0.0033339958581512993
80-81	6.411530496444807E-4	2.564612198577923E-4	0.0	2.28609531381236	0.0034622264680801954
82-83	6.411530496444807E-4	2.564612198577923E-4	0.0	2.49985574056383	0.003718687687937988
84-85	6.411530496444807E-4	2.564612198577923E-4	0.0	2.7095769031025396	0.003718687687937988
86-87	6.411530496444807E-4	2.564612198577923E-4	0.0	2.935775699017112	0.0037828029929024357
88-89	6.411530496444807E-4	2.564612198577923E-4	0.0	3.217626579640826	0.003846918297866884
90-91	6.411530496444807E-4	2.564612198577923E-4	0.0	3.5353820310446307	0.003846918297866884
92-93	6.411530496444807E-4	2.564612198577923E-4	0.0	3.8353775429732835	0.00397514890779578
94-95	6.411530496444807E-4	2.564612198577923E-4	0.0	4.118959536831037	0.00397514890779578
96-97	7.052683546089287E-4	2.564612198577923E-4	0.0	4.42818765267457	0.00397514890779578
98-99	7.693836595733767E-4	2.564612198577923E-4	0.0	4.797299463354898	0.00397514890779578
100-101	7.693836595733767E-4	2.564612198577923E-4	0.0	5.2137283690989875	0.00397514890779578
102-103	7.693836595733767E-4	2.564612198577923E-4	0.0	5.651379440786311	0.004167494822689124
104-105	7.693836595733767E-4	2.564612198577923E-4	0.0	6.071591149523302	0.00429572543261802
106-107	7.693836595733767E-4	2.564612198577923E-4	0.0	6.511806833409203	0.0043598407375824684
108-109	7.693836595733767E-4	2.564612198577923E-4	0.0	6.960229276330553	0.0043598407375824684
110-111	7.693836595733767E-4	2.564612198577923E-4	0.0	7.494566227904263	0.0043598407375824684
112-113	7.693836595733767E-4	2.564612198577923E-4	0.0	8.034737672229738	0.0043598407375824684
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	755	0.0	59.90266	1
GTATCAA	1235	0.0	48.666924	1
TCAACGC	1315	0.0	45.25076	4
CAACGCA	1405	0.0	42.775654	5
GTACATG	3950	0.0	42.18328	1
TACATGG	4070	0.0	40.059704	2
AACGCAG	1530	0.0	39.667286	6
ATCAACG	1555	0.0	38.649384	3
ACATGGG	4150	0.0	38.140392	3
TATCAAC	1725	0.0	34.495506	2
GTATCGC	70	0.0027882247	34.00053	6
CATGGGG	3520	0.0	33.64047	4
ATGGGGG	2000	0.0	31.835037	5
AGTACAT	2575	0.0	30.503405	2
GAGTACA	2685	0.0	30.363777	1
ACGCAGA	2020	0.0	29.452127	7
CTATGCG	125	4.884372E-5	28.556784	9
CGCAGAG	2105	0.0	28.26285	8
GTATTAG	235	3.4015102E-10	27.855072	1
TAGAGCG	115	0.0010040547	25.87163	5
>>END_MODULE