FastQCFastQC Report
Mon 15 Aug 2016
SRR2921282_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921282_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences779845
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15770.20221967185786918No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10700.13720675262391885No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8890.11399701222678865No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9750.077.23261
GTATCAA16900.053.044361
GTACATG34450.043.7165641
TACATGG36500.041.473852
ACATGGG38550.039.113763
TCAACGC22050.038.380734
CAACGCA23000.036.277195
AACGCAG23300.036.045056
CATGGGG32700.035.9047554
TATCAAC24550.035.6861232
ATCAACG24250.035.39033
ATGGGGG21050.034.2583735
TGGTATC4300.030.4921262
GAGTACA23200.029.6240581
GTGGTAT4550.028.896551
ACGCAGA30250.027.9533587
AGTACAT24150.027.3929822
CGCAGAG31900.026.502398
TAGGACA2757.2759576E-1126.0065084
GAGTACT20800.024.89213812-13
Produced by FastQC (version 0.11.5)