##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921282_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	779845
Sequences flagged as poor quality	0
Sequence length	125
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.361076880662182	31.0	30.0	34.0	22.0	34.0
2	30.104243792035597	33.0	31.0	34.0	26.0	34.0
3	30.344989068340503	33.0	31.0	34.0	27.0	34.0
4	32.568447576120896	37.0	35.0	37.0	19.0	37.0
5	32.2600555238541	37.0	33.0	37.0	19.0	37.0
6	32.39738922478185	37.0	35.0	37.0	17.0	37.0
7	32.60983913469985	37.0	35.0	37.0	17.0	37.0
8	32.84819419243568	37.0	35.0	37.0	17.0	37.0
9	34.58389808231123	39.0	37.0	39.0	19.0	39.0
10-11	34.768142387269265	39.0	37.0	39.0	25.5	39.0
12-13	34.79161371811065	39.0	37.0	39.0	26.5	39.0
14-15	36.20655322532042	41.0	38.0	41.0	27.0	41.0
16-17	36.20846386140836	41.0	38.0	41.0	27.0	41.0
18-19	36.24271233386122	41.0	38.0	41.0	27.0	41.0
20-21	36.23968288570164	41.0	38.0	41.0	27.0	41.0
22-23	36.19451172989504	41.0	38.0	41.0	27.0	41.0
24-25	36.14514550968462	41.0	38.0	41.0	27.0	41.0
26-27	36.045217318826175	40.5	38.0	41.0	26.0	41.0
28-29	35.96876558803352	40.0	38.0	41.0	25.0	41.0
30-31	35.86529951464714	40.0	37.0	41.0	25.0	41.0
32-33	35.72208515794806	40.0	37.0	41.0	24.0	41.0
34-35	35.631864665414284	40.0	37.0	41.0	24.0	41.0
36-37	35.546088645820646	40.0	36.5	41.0	23.5	41.0
38-39	35.450041354371706	40.0	36.0	41.0	22.5	41.0
40-41	35.30256589450468	40.0	36.0	41.0	20.5	41.0
42-43	35.14724592707525	40.0	35.5	41.0	18.5	41.0
44-45	34.979280498047686	40.0	35.0	41.0	15.0	41.0
46-47	34.74288993325597	40.0	35.0	41.0	9.5	41.0
48-49	34.501827927344536	40.0	35.0	41.0	6.5	41.0
50-51	34.29817079034936	40.0	35.0	41.0	2.0	41.0
52-53	34.10885175900339	39.0	35.0	41.0	2.0	41.0
54-55	33.90517987548807	39.0	35.0	41.0	2.0	41.0
56-57	33.65791150805609	39.0	34.5	41.0	2.0	41.0
58-59	33.59708660054241	38.5	35.0	41.0	2.0	41.0
60-61	33.35384018619085	38.0	34.5	41.0	2.0	41.0
62-63	33.06882265065494	37.0	34.0	40.0	2.0	41.0
64-65	32.77398713846982	37.0	34.0	40.0	2.0	41.0
66-67	32.46187832197424	36.0	34.0	39.0	2.0	41.0
68-69	32.13596548031981	36.0	34.0	39.0	2.0	41.0
70-71	31.812316550083672	35.0	33.5	38.5	2.0	40.5
72-73	31.469415076072806	35.0	33.0	37.0	2.0	39.5
74-75	31.14329898890164	35.0	33.0	37.0	2.0	39.0
76-77	30.8566292019568	35.0	33.0	36.5	2.0	39.0
78-79	30.59455596945547	35.0	33.0	36.0	2.0	38.0
80-81	30.364021696619197	35.0	33.0	36.0	2.0	37.0
82-83	30.162638088338067	35.0	32.5	35.0	2.0	37.0
84-85	29.98497393712853	35.0	32.0	35.0	2.0	36.0
86-87	29.836957344087608	35.0	32.0	35.0	2.0	36.0
88-89	29.71556334912707	35.0	32.0	35.0	2.0	36.0
90-91	29.60873122223006	35.0	32.0	35.0	2.0	35.5
92-93	29.54337400380845	35.0	32.0	35.0	2.0	35.0
94-95	29.46243356051523	35.0	32.0	35.0	2.0	35.0
96-97	29.393821849213623	35.0	32.0	35.0	2.0	35.0
98-99	29.339427706787887	35.0	32.0	35.0	2.0	35.0
100-101	29.282606800069246	35.0	31.5	35.0	2.0	35.0
102-103	28.60834717155332	34.0	30.0	35.0	2.0	35.0
104-105	29.151795549115526	35.0	31.0	35.0	2.0	35.0
106-107	29.20752200757843	35.0	31.0	35.0	2.0	35.0
108-109	29.28000500099379	35.0	32.0	35.0	2.0	35.0
110-111	29.3902557559515	35.0	32.0	35.0	2.0	35.0
112-113	29.358567407625873	35.0	32.0	35.0	2.0	35.0
114-115	29.302494085363115	35.0	32.0	35.0	2.0	35.0
116-117	29.247947348511566	35.0	32.0	35.0	2.0	35.0
118-119	29.17651969301592	35.0	32.0	35.0	2.0	35.0
120-121	29.08517397688002	35.0	32.0	35.0	2.0	35.0
122-123	28.96995813270586	35.0	32.0	35.0	2.0	35.0
124-125	28.455498849130276	34.5	30.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	48913.0
3	3329.0
4	2412.0
5	2358.0
6	2441.0
7	2524.0
8	2669.0
9	2542.0
10	2834.0
11	2898.0
12	3158.0
13	4063.0
14	4973.0
15	4561.0
16	2892.0
17	2504.0
18	2149.0
19	1790.0
20	1794.0
21	1837.0
22	1998.0
23	2620.0
24	3252.0
25	4250.0
26	4999.0
27	5656.0
28	6937.0
29	8214.0
30	9846.0
31	12234.0
32	15638.0
33	20963.0
34	32241.0
35	55791.0
36	127494.0
37	266963.0
38	97838.0
39	270.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.25730961044576	17.03096773529457	10.411144679077093	27.300577975182577
2	16.463399778161044	19.175477178157198	36.337862011040656	28.023261032641102
3	20.42546916374408	21.901788175855458	28.037494630343208	29.635248030057255
4	13.264205510987988	15.093207133375655	31.49266077990035	40.149926575736
5	13.554364288714781	37.908491694318016	33.06343560157905	15.473708415388154
6	34.90450452299731	33.679042312572406	15.688148605254144	15.728304559176149
7	30.84676022953772	31.624284618561564	17.753003255290167	19.775951896610547
8	29.184321490494607	34.224025607616625	17.35721394055668	19.23443896133209
9	27.888199952551023	15.262925037029436	17.183710894669684	39.66516411574986
10-11	27.62926905582905	25.238097680639427	24.92450248580775	22.208130777723778
12-13	26.80290551060378	22.31094967959965	26.04977704446473	24.83636776533184
14-15	24.04811212484532	23.670216517384866	23.317646455385365	28.96402490238445
16-17	24.35381389891581	26.936186036968884	24.115433195057992	24.594566869057314
18-19	24.22192871660394	25.884502689637046	26.151671165423902	23.74189742833512
20-21	24.708627996589065	24.855067353127865	26.452179599792263	23.984125050490803
22-23	24.516602658220542	24.429982881213576	26.38985952336682	24.66355493719906
24-25	24.08343965788073	24.794221928716603	26.484493713494352	24.637844699908314
26-27	23.906289070263963	24.969256711269548	26.40845296180651	24.71600125665998
28-29	23.953670280632693	25.51660906975104	26.27406728260103	24.25565336701524
30-31	24.50429251966737	25.083061377581444	26.33632324372151	24.076322859029677
32-33	24.025799998717694	25.140508690829588	26.458527014983744	24.37516429546897
34-35	24.109983394136016	25.27919009546769	26.66151607050119	23.949310439895108
36-37	24.27911956863223	25.211933140559985	26.551814783707016	23.95713250710077
38-39	24.26187255159679	25.4638421737653	26.46506677609012	23.80921849854779
40-41	24.171405856291955	25.372798440715783	26.39716866813277	24.05862703485949
42-43	24.030800992504922	25.36523283472998	26.181869474062154	24.422096698702948
44-45	23.751899415909573	25.58598182972257	26.465964390359627	24.196154364008233
46-47	23.85031640903	25.477049926587974	25.96631381877168	24.706319845610345
48-49	23.96431342125679	25.669588187396215	25.74998877982163	24.616109611525367
50-51	24.063307452121897	25.864626945098063	25.774032019183302	24.29803358359674
52-53	24.184421263199738	25.83615974969385	25.78659861895633	24.19282036815008
54-55	23.778571382774782	25.538344158133985	26.135257647353	24.54782681173823
56-57	23.954183203072404	25.73126711077201	25.68523232180754	24.629317364348044
58-59	23.91218767832069	25.711840173367783	25.966249703466715	24.40972244484481
60-61	24.269438157582595	25.625797434105497	26.088774051253775	24.015990357058133
62-63	24.059332303214102	25.735819297424488	26.0061294231546	24.19871897620681
64-65	23.94059075841994	25.259186120318784	26.11345844366509	24.68676467759619
66-67	23.87570606979592	25.62829793099911	25.95535010162276	24.540645897582213
68-69	23.67419166629266	25.59072636228994	26.13307772698421	24.60200424443319
70-71	23.804473965980417	25.421846649013585	26.103199994870774	24.67047939013522
72-73	23.82826074412223	25.963941552487995	25.730433611807474	24.477364091582302
74-75	23.603793061441696	25.92726759804833	25.847508158672554	24.621431181837416
76-77	23.69836313626426	25.48487199379364	25.888670184459734	24.92809468548237
78-79	23.63828709551257	25.44249177721214	26.159685578544455	24.759535548730838
80-81	23.73542178253371	25.723060351736564	25.764414723438634	24.777103142291097
82-83	23.83262058485981	25.803845635991767	25.810577743013035	24.552956036135388
84-85	23.962838769242605	25.8337233681052	25.573671691169398	24.629766171482796
86-87	23.390609672434906	26.075566298431095	25.794292455552064	24.739531573581928
88-89	23.421833825952593	25.69690130731107	25.874885393892377	25.006379472843964
90-91	23.64785197010935	25.458858679797526	25.987619294921732	24.9056700551714
92-93	23.647531392556832	25.78527074377198	25.83842250197957	24.728775361691625
94-95	23.61388944455558	25.598453023938124	25.75098353003934	25.036674001466956
96-97	23.410304003693053	25.57279194196264	25.836499036664456	25.180405017679853
98-99	23.347346810806513	25.631776591358918	25.661718457974636	25.359158139859932
100-101	23.40170162019376	25.685296437112505	25.79781879732511	25.11518314536863
102-103	23.116580859016857	25.699594150119577	25.87866819688528	25.30515679397829
104-105	23.265471513872317	25.7219868832825	25.75032586624641	25.26221573659877
106-107	23.36853484064431	25.541791397889448	25.75753981407808	25.332133947388165
108-109	23.323970319609817	25.577569288118163	25.785560270901204	25.312900121370816
110-111	23.1339168703404	25.79233998681784	25.765026761831244	25.30871638101051
112-113	23.185121021852602	25.809125863880787	25.70564289913873	25.300110215127873
114-115	23.002351758433118	25.943652688621544	25.720081760151416	25.33391379279393
116-117	22.93459102802564	25.690093923172654	25.789280999487712	25.586034049313994
118-119	22.9633547799716	25.530219242988167	25.86874913844783	25.63767683859241
120-121	22.90094102214098	25.683550609833894	25.93783239842353	25.47767596960159
122-123	22.8861325989876	25.784831999179307	25.722382691154834	25.606652710678258
124-125	22.551269935102322	25.76826361203473	25.642597292019037	26.03786916084391
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	18.0
1	20.5
2	35.5
3	43.5
4	34.5
5	45.5
6	116.0
7	241.5
8	340.0
9	425.0
10	462.5
11	427.0
12	416.5
13	469.5
14	522.0
15	554.0
16	610.5
17	682.5
18	731.0
19	736.0
20	794.0
21	869.5
22	912.5
23	1056.0
24	1256.0
25	1402.5
26	1731.5
27	2244.0
28	2639.5
29	3110.0
30	3862.5
31	4810.0
32	6136.5
33	7437.5
34	8562.0
35	9870.0
36	11636.5
37	13308.5
38	14266.5
39	15354.0
40	16541.0
41	17689.5
42	19043.5
43	20882.0
44	22972.0
45	24841.0
46	26564.0
47	28378.5
48	30673.0
49	33091.0
50	34380.5
51	34901.0
52	35002.0
53	34765.0
54	34664.0
55	33704.0
56	31701.0
57	28990.5
58	26379.0
59	23973.0
60	20900.5
61	17694.0
62	14894.5
63	11785.0
64	8687.0
65	6452.5
66	4846.0
67	3665.5
68	2904.0
69	2286.5
70	1770.5
71	1410.0
72	1099.0
73	914.5
74	762.5
75	579.0
76	417.0
77	278.0
78	194.0
79	146.5
80	124.0
81	102.0
82	74.0
83	59.0
84	58.5
85	56.0
86	46.0
87	48.0
88	43.0
89	32.5
90	27.5
91	26.0
92	31.0
93	29.5
94	20.5
95	17.5
96	18.0
97	15.0
98	9.0
99	2.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.33968288570164584
2	0.0
3	0.0
4	0.016669979290756497
5	0.17977931512031237
6	0.04924055421269612
7	0.02449204649641916
8	0.009873756964525002
9	0.007693836595733768
10-11	0.0023081509787201302
12-13	0.05507504696446088
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	3.2057652482224034E-4
92-93	3.2057652482224034E-4
94-95	1.2823060992889614E-4
96-97	3.2057652482224034E-4
98-99	6.411530496444807E-5
100-101	0.0
102-103	0.0
104-105	6.411530496444807E-5
106-107	1.923459148933442E-4
108-109	4.488071347511365E-4
110-111	3.846918297866884E-4
112-113	8.334989645378248E-4
114-115	3.846918297866884E-4
116-117	7.052683546089287E-4
118-119	3.2057652482224034E-4
120-121	1.923459148933442E-4
122-123	0.0016028826241112017
124-125	2.564612198577923E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	779845.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	71.17510702178166
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.35327890555396	60.750287607627826
2	8.602999947449048	12.246388839361359
3	2.4746454830834645	5.2839947129830245
4	1.157652403780649	3.2958413493244194
5	0.6108611509419144	2.1739053896869738
6	0.4154617808546484	1.7742322029473758
7	0.28860160473477975	1.4378875072559114
8	0.1944108295506656	1.1069769279569581
9	0.15134264779979079	0.9694646238698926
>10	0.7221100684160059	8.780690671055611
>50	0.021972057805476865	1.0641804423577128
>100	0.006122867065541522	0.6629221885689206
>500	1.8008432131969606E-4	0.11394776029315237
>1k	3.601686426393921E-4	0.3392797767108823
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1577	0.20221967185786918	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1070	0.13720675262391885	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	889	0.11399701222678865	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.2823060992889614E-4
2	0.0	0.0	0.0	0.0	1.2823060992889614E-4
3	0.0	0.0	0.0	0.0	1.2823060992889614E-4
4	0.0	0.0	0.0	0.0	1.2823060992889614E-4
5	0.0	0.0	0.0	0.0	1.2823060992889614E-4
6	0.0	0.0	0.0	0.0	1.2823060992889614E-4
7	0.0	0.0	0.0	0.0	1.2823060992889614E-4
8	0.0	0.0	0.0	0.0	1.2823060992889614E-4
9	0.0	0.0	0.0	0.0	2.564612198577923E-4
10-11	0.0	0.0	0.0	0.0	2.564612198577923E-4
12-13	0.0	0.0	0.0	0.0	2.564612198577923E-4
14-15	0.0	0.0	0.0	0.0	3.846918297866884E-4
16-17	0.0	0.0	0.0	0.0	7.693836595733768E-4
18-19	0.0	0.0	0.0	0.0	0.001025844879431169
20-21	0.0	0.0	0.0	0.0	0.001025844879431169
22-23	0.0	0.0	0.0	6.411530496444807E-5	0.0013464214042534094
24-25	0.0	0.0	0.0	2.564612198577923E-4	0.0016028826241112014
26-27	0.0	0.0	0.0	7.693836595733768E-4	0.0016669979290756497
28-29	0.0	0.0	0.0	0.002885188723400163	0.0017952285390045457
30-31	0.0	0.0	0.0	0.009489065134738314	0.0017952285390045457
32-33	0.0	0.0	0.0	0.018529323134725488	0.0017952285390045457
34-35	0.0	0.0	0.0	0.028659541319108285	0.001923459148933442
36-37	0.0	0.0	0.0	0.04462425225525585	0.001923459148933442
38-39	0.0	0.0	0.0	0.07822067205662664	0.00198757445389789
40-41	0.0	0.0	0.0	0.12797414870903834	0.002051689758862338
42-43	0.0	0.0	0.0	0.16830267553167616	0.002051689758862338
44-45	0.0	0.0	0.0	0.21260635126210978	0.002051689758862338
46-47	0.0	0.0	0.0	0.2615263289499836	0.002051689758862338
48-49	0.0	0.0	0.0	0.31320326475132876	0.002051689758862338
50-51	0.0	0.0	0.0	0.37430515038244777	0.0021799203687912342
52-53	0.0	0.0	0.0	0.438869262481647	0.0024363815886490267
54-55	0.0	0.0	0.0	0.5037539511056685	0.0026928428085068188
56-57	0.0	1.2823060992889614E-4	0.0	0.582679891516904	0.0026928428085068188
58-59	0.0	1.2823060992889614E-4	0.0	0.6681455930345133	0.0026928428085068188
60-61	0.0	1.2823060992889614E-4	0.0	0.7651520494457231	0.002821073418435715
62-63	0.0	1.2823060992889614E-4	0.0	0.8742121831902494	0.003077534638293507
64-65	0.0	1.2823060992889614E-4	0.0	0.970321025331957	0.003077534638293507
66-67	0.0	2.564612198577923E-4	0.0	1.0832921926793144	0.003077534638293507
68-69	1.2823060992889614E-4	2.564612198577923E-4	0.0	1.207419423090486	0.0032057652482224033
70-71	1.2823060992889614E-4	2.564612198577923E-4	0.0	1.349370708281774	0.0032057652482224033
72-73	1.2823060992889614E-4	2.564612198577923E-4	0.0	1.4845898864517948	0.004103379517724676
74-75	1.2823060992889614E-4	2.564612198577923E-4	0.0	1.6260282492033673	0.004231610127653572
76-77	1.2823060992889614E-4	2.564612198577923E-4	0.0	1.7943309247350436	0.00429572543261802
78-79	1.2823060992889614E-4	2.564612198577923E-4	0.0	1.98815149164257	0.0043598407375824684
80-81	1.2823060992889614E-4	2.564612198577923E-4	0.0	2.1791509851316606	0.0043598407375824684
82-83	1.2823060992889614E-4	2.564612198577923E-4	0.0	2.380152466195206	0.0043598407375824684
84-85	1.2823060992889614E-4	2.564612198577923E-4	0.0	2.5779481820105277	0.0043598407375824684
86-87	1.2823060992889614E-4	2.564612198577923E-4	0.0	2.792990914861287	0.0043598407375824684
88-89	1.2823060992889614E-4	2.564612198577923E-4	0.0	3.0625957722367905	0.0043598407375824684
90-91	1.2823060992889614E-4	2.564612198577923E-4	0.0	3.36355301373991	0.0043598407375824684
92-93	1.2823060992889614E-4	2.564612198577923E-4	0.0	3.649635504491277	0.0043598407375824684
94-95	1.2823060992889614E-4	2.564612198577923E-4	0.0	3.9173810180228124	0.004488071347511364
96-97	1.2823060992889614E-4	2.564612198577923E-4	0.0	4.214234880008206	0.004552186652475813
98-99	1.2823060992889614E-4	2.564612198577923E-4	0.0	4.567959017497067	0.0046163019574402605
100-101	1.2823060992889614E-4	2.564612198577923E-4	0.0	4.964832755227	0.0046163019574402605
102-103	1.2823060992889614E-4	2.564612198577923E-4	0.0	5.37779943450301	0.0048727631772980525
104-105	1.2823060992889614E-4	2.564612198577923E-4	0.0	5.777494245651379	0.0048727631772980525
106-107	1.2823060992889614E-4	2.564612198577923E-4	0.0	6.199629413537306	0.0048727631772980525
108-109	1.2823060992889614E-4	2.564612198577923E-4	0.0	6.633754143451584	0.0048727631772980525
110-111	1.2823060992889614E-4	2.564612198577923E-4	0.0	7.1459713148125585	0.004936878482262501
112-113	1.2823060992889614E-4	2.564612198577923E-4	0.0	7.66562586154941	0.005000993787226949
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	975	0.0	77.2326	1
GTATCAA	1690	0.0	53.04436	1
GTACATG	3445	0.0	43.716564	1
TACATGG	3650	0.0	41.47385	2
ACATGGG	3855	0.0	39.11376	3
TCAACGC	2205	0.0	38.38073	4
CAACGCA	2300	0.0	36.27719	5
AACGCAG	2330	0.0	36.04505	6
CATGGGG	3270	0.0	35.904755	4
TATCAAC	2455	0.0	35.686123	2
ATCAACG	2425	0.0	35.3903	3
ATGGGGG	2105	0.0	34.258373	5
TGGTATC	430	0.0	30.492126	2
GAGTACA	2320	0.0	29.624058	1
GTGGTAT	455	0.0	28.89655	1
ACGCAGA	3025	0.0	27.953358	7
AGTACAT	2415	0.0	27.392982	2
CGCAGAG	3190	0.0	26.50239	8
TAGGACA	275	7.2759576E-11	26.006508	4
GAGTACT	2080	0.0	24.892138	12-13
>>END_MODULE