FastQCFastQC Report
Mon 15 Aug 2016
SRR2921203_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921203_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3235600
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17300.014.2262021
GTATCAA38450.010.6022061
TCGATAA1303.8596409E-710.22802318
CCAATCG856.6086586E-410.056917
TACGTAA1054.1165804E-59.95047813
GTCTTAT2251.8189894E-129.7136091
GGTTAGG1304.228141E-69.5024431
TCTTATA3100.09.5008282
AAATTCG1701.672015E-89.49818314
AGGGTCG1502.661218E-79.497455
TAGACCC1304.25737E-69.4973034
TAAGACT3200.09.4973034
TAGACAG3450.09.3598065
CTAGACA3250.09.3511914
GTATTAT2451.8189894E-129.3085161
TCTACAC4100.09.2660913
TTCGATA1651.0698568E-79.2103617
GTCCTAA2800.09.163071
GTCTAGG2800.09.163071
GATCGCA1252.7621034E-59.11825711
Produced by FastQC (version 0.11.5)