##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921203_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3235600
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.217878291506985	31.0	28.0	31.0	10.0	34.0
2	27.0356626282606	31.0	27.0	31.0	10.0	34.0
3	26.79986494004203	31.0	26.0	31.0	10.0	34.0
4	30.718390097663494	35.0	31.0	37.0	10.0	37.0
5	30.39101619483249	35.0	30.0	37.0	10.0	37.0
6	30.764702682655457	35.0	32.0	37.0	10.0	37.0
7	30.713634256397576	35.0	32.0	37.0	10.0	37.0
8	30.322225244158734	35.0	30.0	37.0	8.0	37.0
9	31.980377364321917	37.0	32.0	39.0	8.0	39.0
10	31.33286747434788	37.0	30.0	39.0	7.0	39.0
11	31.78131382123872	37.0	32.0	39.0	2.0	39.0
12	31.47554085795525	37.0	31.0	39.0	2.0	39.0
13	31.492087711707256	37.0	31.0	39.0	2.0	39.0
14	32.59768111014959	38.0	32.0	40.0	2.0	41.0
15	32.24645598961553	38.0	31.0	40.0	2.0	41.0
16	32.16071764124119	38.0	31.0	40.0	2.0	41.0
17	32.47854741006305	38.0	32.0	40.0	2.0	41.0
18	32.53089504265051	38.0	32.0	40.0	2.0	41.0
19	32.23989739151935	38.0	31.0	40.0	2.0	41.0
20	32.100760909877614	38.0	31.0	40.0	2.0	41.0
21	32.056458771170725	38.0	31.0	40.0	2.0	41.0
22	31.27349919643961	38.0	30.0	40.0	2.0	41.0
23	31.38605390035851	38.0	30.0	40.0	2.0	41.0
24	31.031239955495117	38.0	30.0	40.0	2.0	41.0
25	30.52931604648288	38.0	29.0	40.0	2.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	257140.0
3	7.0
4	2235.0
5	12363.0
6	14441.0
7	12736.0
8	10892.0
9	10181.0
10	9572.0
11	9125.0
12	8766.0
13	8561.0
14	8621.0
15	8876.0
16	9256.0
17	10412.0
18	11924.0
19	14074.0
20	17962.0
21	22678.0
22	27562.0
23	32239.0
24	37382.0
25	42578.0
26	47638.0
27	53259.0
28	59237.0
29	69612.0
30	82305.0
31	97886.0
32	122910.0
33	155250.0
34	199533.0
35	258814.0
36	342788.0
37	456728.0
38	690057.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.968311665184196	17.15900374435263	12.204314090310286	25.668370500152882
2	16.218827074216364	19.038274576547916	38.79357901429112	25.949319334944597
3	19.021843790638147	22.813495813276035	29.134306840546543	29.030353555539275
4	13.740212862040263	15.236515610429022	34.502004642003484	36.52126688552723
5	14.268171119391337	36.16948738673718	33.960810253192534	15.60153124067895
6	34.86862926415575	33.23442255722011	17.00077513246482	14.896173046159324
7	30.195921470693683	31.154419127919198	19.917075673470396	18.732583727916722
8	26.801343700269165	33.64501723512869	20.05453180501696	19.499107259585177
9	27.312525885676493	15.231379312476431	18.552782053421854	38.90331274842522
10	16.252854670718815	27.772882413064863	32.324438330303906	23.649824585912416
11	36.13566964563073	22.05124749593616	21.653750060660087	20.159332797773025
12	24.020989655389844	24.154114538708523	28.697113226932338	23.127782578969295
13	29.234318954179678	20.706290345263504	24.696022538484957	25.363368162071858
14	23.312038789515384	20.099990695808263	25.238841074822705	31.349129439853655
15	25.030668854568894	27.30861753859763	22.516147318063144	25.14456628877033
16	24.726601567753313	26.517506990893676	24.235994639155496	24.519896802197515
17	23.899851374928772	26.359508186127933	25.283669205794247	24.456971233149048
18	23.720221240141758	26.32760159957661	25.85070247330948	24.101474686972153
19	25.048059253141126	25.585605747512652	25.917747040325878	23.448587959020347
20	25.713263884531294	25.130993143010112	25.310555214332325	23.845187758126272
21	26.037954460582707	24.83631257836593	24.88617618763803	24.23955677341333
22	25.014511262532384	24.3200105828675	26.244429622385734	24.421048532214385
23	25.29204096223985	24.584700766009586	25.225983801931147	24.89727446981942
24	24.847018589594388	24.928126478851212	25.506854760312898	24.718000171241503
25	24.24890984806803	24.743281795114775	26.103627464252583	24.90418089256461
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1137.0
1	1137.0
2	1215.0
3	1293.0
4	1293.0
5	1293.0
6	1723.0
7	2153.0
8	2153.0
9	2153.0
10	2810.5
11	3468.0
12	3468.0
13	3468.0
14	5100.0
15	6732.0
16	6732.0
17	6732.0
18	12106.5
19	17481.0
20	17481.0
21	17481.0
22	28710.5
23	39940.0
24	39940.0
25	39940.0
26	60915.5
27	81891.0
28	81891.0
29	81891.0
30	110218.0
31	138545.0
32	138545.0
33	138545.0
34	170256.0
35	201967.0
36	201967.0
37	201967.0
38	243186.0
39	284405.0
40	284405.0
41	284405.0
42	324105.0
43	363805.0
44	363805.0
45	363805.0
46	396419.0
47	429033.0
48	429033.0
49	429033.0
50	441972.5
51	454912.0
52	454912.0
53	454912.0
54	435858.0
55	416804.0
56	416804.0
57	416804.0
58	374405.0
59	332006.0
60	332006.0
61	332006.0
62	281146.0
63	230286.0
64	230286.0
65	230286.0
66	180875.0
67	131464.0
68	131464.0
69	131464.0
70	96210.0
71	60956.0
72	60956.0
73	60956.0
74	42623.0
75	24290.0
76	24290.0
77	24290.0
78	16624.5
79	8959.0
80	8959.0
81	8959.0
82	5972.5
83	2986.0
84	2986.0
85	2986.0
86	1930.0
87	874.0
88	874.0
89	874.0
90	527.0
91	180.0
92	180.0
93	180.0
94	99.0
95	18.0
96	18.0
97	18.0
98	16.5
99	15.0
100	15.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03489306465570528
2	0.05266411175670664
3	0.015236741253554209
4	0.01109531462479911
5	0.009735443194461615
6	7.417480529113611E-4
7	0.0037087402645568056
8	0.0017616516256644826
9	0.0067993571516874775
10	0.0034923970824576583
11	0.01109531462479911
12	0.00902460131042156
13	0.0075720113734701455
14	0.01542217826678205
15	0.007201137347014465
16	0.03198788478180245
17	0.03977623933737174
18	0.022994189640252197
19	0.016411175670663865
20	0.0156694276177525
21	0.02398318704413401
22	0.005624922734577822
23	0.01616392631969341
24	0.012455186055136604
25	0.46198541228829276
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3235600.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.92482793839197
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.42097351775581	29.369585156269423
2	12.262120266563317	10.52698805204199
3	6.0729501683789495	7.8204102316828505
4	3.5973546265350755	6.176633135095858
5	2.31638151039088	4.971513888660052
6	1.553460726029292	4.00092206243141
7	1.131848857536231	3.400909222340578
8	0.8335912026566019	2.8625407155993496
9	0.6364988013001942	2.4589441375923218
>10	3.034309920080069	21.713241896967805
>50	0.10168188273960552	2.95596400365493
>100	0.036286504862150265	2.6742024060946856
>500	0.00171959849878735	0.49763115685688686
>1k	8.224166728771609E-4	0.5705139347118656
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.090616887130671E-4
2	0.0	0.0	0.0	0.0	3.090616887130671E-4
3	0.0	0.0	0.0	0.0	3.090616887130671E-4
4	0.0	0.0	0.0	0.0	3.090616887130671E-4
5	0.0	0.0	0.0	0.0	3.090616887130671E-4
6	0.0	0.0	0.0	0.0	4.3268636419829397E-4
7	0.0	0.0	0.0	0.0	4.635925330696007E-4
8	0.0	0.0	0.0	0.0	5.254048708122142E-4
9	0.0	0.0	0.0	3.0906168871306714E-5	6.799357151687477E-4
10	0.0	0.0	0.0	3.0906168871306714E-5	6.799357151687477E-4
11	0.0	0.0	0.0	3.0906168871306714E-5	7.108418840400544E-4
12	0.0	0.0	0.0	3.0906168871306714E-5	0.0012053405859809617
13	0.0	0.0	0.0	3.0906168871306714E-5	0.0014525899369514155
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1730	0.0	14.226202	1
GTATCAA	3845	0.0	10.602206	1
TCGATAA	130	3.8596409E-7	10.228023	18
CCAATCG	85	6.6086586E-4	10.0569	17
TACGTAA	105	4.1165804E-5	9.950478	13
GTCTTAT	225	1.8189894E-12	9.713609	1
GGTTAGG	130	4.228141E-6	9.502443	1
TCTTATA	310	0.0	9.500828	2
AAATTCG	170	1.672015E-8	9.498183	14
AGGGTCG	150	2.661218E-7	9.49745	5
TAGACCC	130	4.25737E-6	9.497303	4
TAAGACT	320	0.0	9.497303	4
TAGACAG	345	0.0	9.359806	5
CTAGACA	325	0.0	9.351191	4
GTATTAT	245	1.8189894E-12	9.308516	1
TCTACAC	410	0.0	9.266091	3
TTCGATA	165	1.0698568E-7	9.21036	17
GTCCTAA	280	0.0	9.16307	1
GTCTAGG	280	0.0	9.16307	1
GATCGCA	125	2.7621034E-5	9.118257	11
>>END_MODULE