FastQCFastQC Report
Mon 15 Aug 2016
SRR2921203_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921203_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3235600
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT52750.16303004079614292No Hit
GGTATCAACGCAGAGTACTTTTTTT34340.10613178390406725No Hit
TATCAACGCAGAGTACTTTTTTTTT33970.10498825565582891No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21150.014.4398431
TAGACCG1006.6211214E-1014.2493045
TTAGGGT1850.011.8160824
CTAAGAC2550.010.4363513
CGATTCG1502.4485416E-810.1331529
CCCTATA2450.010.0864442
GTATCAA42950.010.0789671
GGCGTAG951.6206304E-410.0148441
GTACCGC1451.635035E-79.820886
TCGCTCG1856.566552E-109.75085218
TGCACCG2151.0913936E-119.7204555
GTTCTAG2350.09.716531
GTCTTAG3200.09.5141021
CTCCTAG4300.09.5141011
TTCGATC1002.728841E-49.50968419
CCGTAAA1201.6920616E-59.5045332
CGTAAAC1201.6920616E-59.5045333
ACGATTC1951.6880222E-99.2550998
TTACGAT1752.7024726E-89.2222736
AGGATCG1651.0675285E-79.2116725
Produced by FastQC (version 0.11.5)