##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921203_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3235600
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.620428668562244	31.0	30.0	33.0	26.0	34.0
2	29.8796303622203	31.0	30.0	34.0	26.0	34.0
3	29.994948386697985	31.0	30.0	34.0	26.0	34.0
4	33.668236184942515	35.0	35.0	37.0	30.0	37.0
5	33.20112622079367	35.0	35.0	37.0	27.0	37.0
6	33.14832952157251	36.0	35.0	37.0	27.0	37.0
7	33.00022376066263	36.0	35.0	37.0	26.0	37.0
8	32.940713005315864	36.0	35.0	37.0	26.0	37.0
9	34.35400791197923	39.0	35.0	39.0	26.0	39.0
10	34.215432995425886	38.0	35.0	39.0	26.0	39.0
11	34.2301780195327	38.0	35.0	39.0	26.0	39.0
12	34.106095623686485	38.0	35.0	39.0	25.0	39.0
13	34.07519934478922	38.0	35.0	39.0	25.0	39.0
14	35.02281709729262	39.0	35.0	41.0	25.0	41.0
15	35.01284800346149	39.0	35.0	41.0	25.0	41.0
16	34.996808938064035	39.0	35.0	41.0	25.0	41.0
17	34.97287890963036	39.0	35.0	41.0	25.0	41.0
18	34.925938620348624	39.0	35.0	41.0	25.0	41.0
19	34.96023735937693	39.0	35.0	41.0	24.0	41.0
20	34.89033718630239	39.0	35.0	41.0	24.0	41.0
21	34.81996816664606	39.0	35.0	41.0	23.0	41.0
22	34.733327976264064	39.0	34.0	41.0	22.0	41.0
23	34.60276672023736	39.0	34.0	41.0	19.0	41.0
24	34.524059525281245	39.0	34.0	41.0	19.0	41.0
25	34.38509766349363	39.0	34.0	41.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	104991.0
3	7.0
4	1714.0
5	14261.0
6	16106.0
7	12096.0
8	9393.0
9	8255.0
10	7393.0
11	6711.0
12	6272.0
13	5958.0
14	5790.0
15	5963.0
16	5904.0
17	6240.0
18	6831.0
19	7904.0
20	10177.0
21	12896.0
22	16610.0
23	20162.0
24	23675.0
25	27806.0
26	31632.0
27	35329.0
28	40683.0
29	49340.0
30	63146.0
31	80071.0
32	101381.0
33	132152.0
34	173176.0
35	229083.0
36	313875.0
37	474249.0
38	1168368.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.41380517985059	18.260962647580943	8.946679974037016	32.378552198531445
2	15.153449128446036	20.383483743355175	34.77243787860057	29.69062924959822
3	18.07236887670799	23.24086762508781	27.334358210727327	31.35240528747687
4	13.556259031604782	15.974819584369143	32.24851512918461	38.220406254841464
5	15.086562726853483	37.076152249443176	32.15955391268226	15.677731111021071
6	35.187953284917	34.11774960497912	15.988269301622863	14.706027808481018
7	30.854919893756232	31.14698517544624	18.983068867448104	19.015026063349424
8	28.20075337844208	34.430482803257696	18.111373529383584	19.257390288916646
9	27.538539992582518	15.200642848312523	18.205402398318704	39.055414760786256
10	17.207256768450982	28.236586722709855	30.573123995549512	23.983032513289654
11	36.52218288461883	22.013190976130996	21.223110975933178	20.241515163316993
12	24.340215106935347	24.288725429595747	28.075101990357275	23.295957473111635
13	29.40496328057559	20.497786882383178	24.478740564385944	25.618509272655288
14	23.471741619996404	19.998694913036985	24.897255327893824	31.63230813907279
15	25.21480989614005	27.387098638973157	22.037895572481705	25.36019589240509
16	25.336396120785174	26.42531967483434	23.409584666116707	24.828699538263773
17	24.062492273457785	26.297008282853255	25.049326245518603	24.591173198170356
18	23.922083911345858	26.231475780347825	25.705824388048555	24.140615920257762
19	25.365427418175816	25.45555757836703	25.409657959751137	23.76935704370602
20	25.833446655952528	24.990913586351834	25.116021757942885	24.05961799975275
21	25.862429799529686	24.682990797389547	24.90436166453828	24.550217738542486
22	25.94928532398399	24.469988799576708	24.651037584466767	24.929688291972536
23	25.341565185139398	24.514681337701674	24.941676454225135	25.202077022933793
24	25.04284193489757	25.0295813360007	24.99289058800401	24.934686141097718
25	24.881062823353677	24.73472148077869	25.12949629594765	25.254719399919985
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	920.0
1	920.0
2	935.0
3	950.0
4	950.0
5	950.0
6	1312.5
7	1675.0
8	1675.0
9	1675.0
10	2264.0
11	2853.0
12	2853.0
13	2853.0
14	4050.5
15	5248.0
16	5248.0
17	5248.0
18	10161.5
19	15075.0
20	15075.0
21	15075.0
22	25476.0
23	35877.0
24	35877.0
25	35877.0
26	55046.0
27	74215.0
28	74215.0
29	74215.0
30	99413.5
31	124612.0
32	124612.0
33	124612.0
34	153269.0
35	181926.0
36	181926.0
37	181926.0
38	222243.0
39	262560.0
40	262560.0
41	262560.0
42	303875.0
43	345190.0
44	345190.0
45	345190.0
46	382121.5
47	419053.0
48	419053.0
49	419053.0
50	441094.0
51	463135.0
52	463135.0
53	463135.0
54	448435.5
55	433736.0
56	433736.0
57	433736.0
58	394191.5
59	354647.0
60	354647.0
61	354647.0
62	303946.5
63	253246.0
64	253246.0
65	253246.0
66	200717.5
67	148189.0
68	148189.0
69	148189.0
70	108959.5
71	69730.0
72	69730.0
73	69730.0
74	48804.5
75	27879.0
76	27879.0
77	27879.0
78	18986.0
79	10093.0
80	10093.0
81	10093.0
82	6823.5
83	3554.0
84	3554.0
85	3554.0
86	2280.5
87	1007.0
88	1007.0
89	1007.0
90	595.0
91	183.0
92	183.0
93	183.0
94	112.5
95	42.0
96	42.0
97	42.0
98	23.5
99	5.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.10217579428853998
2	0.0
3	0.04302138706885895
4	0.056156508839164294
5	0.07760539003585115
6	0.010137223389788601
7	6.799357151687477E-4
8	0.009488193843491161
9	0.0
10	0.0
11	0.011064408455927804
12	0.0
13	0.0036778340956854986
14	0.06487204846087279
15	0.02358140684880702
16	0.0062739522808752625
17	0.0
18	0.011620719495611324
19	0.008622821115094572
20	0.0
21	0.02339596983557918
22	2.472493509704537E-4
23	0.006181233774261343
24	0.013969588329830633
25	0.1905056249227346
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3235600.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.40925418605468
#Duplication Level	Percentage of deduplicated	Percentage of total
1	58.46993734687814	18.364971243707874
2	13.960154825780718	8.769561027992491
3	7.441995566844898	7.012425912315703
4	4.819021950711598	6.054475415123107
5	3.2179086677491773	5.053605564642124
6	2.3048623123900174	4.34364037402295
7	1.7274213538279195	3.797991147215988
8	1.2923989674311098	3.2474630142270664
9	1.0328195086882	2.91960814290334
>10	5.421136813970905	29.30717280918814
>50	0.22636033748047255	4.783506824262516
>100	0.08041161886274599	4.347080634930185
>500	0.003780137796522515	0.8365558064917069
>1k	0.0016911142772359315	0.9971242791771621
>5k	9.947731042564303E-5	0.16481780379969072
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	5275	0.16303004079614292	No Hit
GGTATCAACGCAGAGTACTTTTTTT	3434	0.10613178390406725	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3397	0.10498825565582891	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.271850661392014E-5
2	0.0	0.0	0.0	0.0	9.271850661392014E-5
3	0.0	0.0	0.0	0.0	1.2362467548522686E-4
4	0.0	0.0	0.0	0.0	1.2362467548522686E-4
5	3.0906168871306714E-5	0.0	0.0	0.0	1.2362467548522686E-4
6	3.0906168871306714E-5	0.0	0.0	0.0	3.090616887130671E-4
7	3.0906168871306714E-5	0.0	0.0	0.0	3.090616887130671E-4
8	3.0906168871306714E-5	0.0	0.0	0.0	3.090616887130671E-4
9	3.0906168871306714E-5	0.0	0.0	0.0	3.3996785758437384E-4
10	3.0906168871306714E-5	0.0	0.0	0.0	4.0178019532698724E-4
11	3.0906168871306714E-5	0.0	0.0	0.0	4.3268636419829397E-4
12	3.0906168871306714E-5	0.0	0.0	0.0	0.0012053405859809617
13	3.0906168871306714E-5	0.0	0.0	0.0	0.0013598714303374954
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2115	0.0	14.439843	1
TAGACCG	100	6.6211214E-10	14.249304	5
TTAGGGT	185	0.0	11.816082	4
CTAAGAC	255	0.0	10.436351	3
CGATTCG	150	2.4485416E-8	10.133152	9
CCCTATA	245	0.0	10.086444	2
GTATCAA	4295	0.0	10.078967	1
GGCGTAG	95	1.6206304E-4	10.014844	1
GTACCGC	145	1.635035E-7	9.82088	6
TCGCTCG	185	6.566552E-10	9.750852	18
TGCACCG	215	1.0913936E-11	9.720455	5
GTTCTAG	235	0.0	9.71653	1
GTCTTAG	320	0.0	9.514102	1
CTCCTAG	430	0.0	9.514101	1
TTCGATC	100	2.728841E-4	9.509684	19
CCGTAAA	120	1.6920616E-5	9.504533	2
CGTAAAC	120	1.6920616E-5	9.504533	3
ACGATTC	195	1.6880222E-9	9.255099	8
TTACGAT	175	2.7024726E-8	9.222273	6
AGGATCG	165	1.0675285E-7	9.211672	5
>>END_MODULE