FastQCFastQC Report
Mon 15 Aug 2016
SRR2921202_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921202_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2270687
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT26650.11736536123208527No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG751.4858502E-512.661225
TCTTATA1301.9826984E-1012.4223852
GTTCGCG550.00307199412.08943716
TTGGACT2950.011.5887454
CGGCAAA1354.7439244E-911.25739317
GTATTAG1701.382432E-1010.6189281
GGTATCA15850.010.3103911
TTAGACT1701.5697879E-910.0549414
CTTAGAC2002.5465852E-119.9715883
GTTATCA2201.8189894E-129.9330161
CCGTCCA1151.0320686E-59.908789
TCTATAC1356.4756387E-79.8484833
GTATCAA29950.09.7390711
GTCCTAA2106.730261E-119.5011461
GTCTTGG4500.09.5011451
TCACCGA800.00452051879.49905313
CTAGATC900.00111741929.4967513
ACCGTTC1002.7680252E-49.4959148
GACGCTC1401.0651511E-69.4959148
ACCGTCC1401.0651511E-69.4959148
Produced by FastQC (version 0.11.5)