##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921202_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2270687
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.998044644638384	30.0	26.0	31.0	2.0	34.0
2	25.6265544304433	31.0	25.0	31.0	2.0	34.0
3	25.414884570176337	30.0	25.0	31.0	2.0	34.0
4	29.16594228971232	35.0	28.0	37.0	2.0	37.0
5	28.753785528344505	35.0	28.0	37.0	2.0	37.0
6	29.228763805843784	35.0	29.0	37.0	2.0	37.0
7	29.024735685719783	35.0	29.0	37.0	2.0	37.0
8	28.73166667180461	35.0	28.0	37.0	2.0	37.0
9	30.312982370533675	37.0	30.0	39.0	2.0	39.0
10	29.67165619920315	35.0	27.0	39.0	2.0	39.0
11	30.11191502835926	37.0	29.0	39.0	2.0	39.0
12	29.81651588263816	37.0	28.0	39.0	2.0	39.0
13	29.794295735167374	37.0	27.0	39.0	2.0	39.0
14	30.806322051432012	37.0	29.0	40.0	2.0	41.0
15	30.477994545263172	37.0	27.0	40.0	2.0	41.0
16	30.384327738697582	37.0	27.0	40.0	2.0	41.0
17	30.6724784173248	37.0	29.0	40.0	2.0	41.0
18	30.706121539428377	38.0	29.0	40.0	2.0	41.0
19	30.402253150698446	37.0	27.0	40.0	2.0	41.0
20	30.307772053127533	37.0	27.0	40.0	2.0	41.0
21	30.219681532505362	37.0	27.0	40.0	2.0	41.0
22	29.49438077550979	37.0	25.0	40.0	2.0	41.0
23	29.574316055008904	37.0	27.0	40.0	2.0	41.0
24	29.227683956441375	37.0	25.0	40.0	2.0	41.0
25	28.737565767540836	37.0	23.0	40.0	2.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	265522.0
3	10.0
4	2122.0
5	11067.0
6	12383.0
7	10760.0
8	9150.0
9	8370.0
10	7985.0
11	7570.0
12	7205.0
13	6913.0
14	6964.0
15	7053.0
16	7432.0
17	8136.0
18	9302.0
19	11434.0
20	14067.0
21	17525.0
22	21045.0
23	24585.0
24	28083.0
25	31238.0
26	34543.0
27	39011.0
28	42875.0
29	49633.0
30	58239.0
31	68618.0
32	85928.0
33	108557.0
34	138366.0
35	178725.0
36	231166.0
37	300797.0
38	398308.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.50652687912927	17.465850133110358	12.018391829495387	26.009231158264978
2	15.815874876345875	19.817399484893816	38.82904775858309	25.537677880177224
3	19.79680853922112	23.354464975107593	28.955664074285657	27.89306241138563
4	13.657458690071039	15.498848470727724	34.27381864195742	36.56987419724382
5	14.221071023214005	36.51976253510817	33.426676174188486	15.83249026748934
6	34.03692920456482	33.77850316360509	17.33101566102325	14.85355197080684
7	29.437839528057374	31.300594772781825	19.864133725884837	19.397431973275964
8	27.34873274172594	33.97604210248167	19.926540858344527	18.748684297447866
9	27.272671218608142	15.871867971088726	18.733354679275745	38.12210613102739
10	16.20479290550628	28.014723822627086	32.29631208584845	23.484171186018184
11	36.09461643829583	22.439846196535456	21.58212321014081	19.8834141550279
12	23.793634847363094	24.521265842743443	28.851465237683545	22.83363407220992
13	28.85183966653938	21.244725264513036	24.822230238102474	25.081204830845106
14	23.013384755790312	20.556664540703963	25.460590994140098	30.96935970936563
15	24.96463415063826	27.552595139860266	22.781244384625413	24.701526324876067
16	24.512194907055797	26.759983858774856	24.586733534100098	24.141087700069253
17	23.803649519908994	26.911045926077353	25.31428183725457	23.971022716759084
18	23.733437994591622	26.629330436648	25.97563806586347	23.661593502896906
19	24.717141778622892	25.947548062883126	26.32872999114217	23.006580167351817
20	25.20590591776625	25.357161262246553	25.983324913129486	23.453607906857716
21	25.41418868529589	25.11002523609559	25.520480705993982	23.95530537261454
22	24.551815441992222	24.683061233208356	26.677160457669046	24.087962867130372
23	24.73187759461751	25.002191316221246	25.802473214187394	24.463457874973848
24	24.355813872569122	25.57787689782917	25.83853317201244	24.227776057589267
25	24.007352160338687	25.087167524477707	26.41162717432089	24.49385314086272
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1013.0
1	1013.0
2	1079.0
3	1145.0
4	1145.0
5	1145.0
6	1588.5
7	2032.0
8	2032.0
9	2032.0
10	2622.5
11	3213.0
12	3213.0
13	3213.0
14	4764.5
15	6316.0
16	6316.0
17	6316.0
18	10808.0
19	15300.0
20	15300.0
21	15300.0
22	23729.5
23	32159.0
24	32159.0
25	32159.0
26	47625.5
27	63092.0
28	63092.0
29	63092.0
30	83218.0
31	103344.0
32	103344.0
33	103344.0
34	127589.5
35	151835.0
36	151835.0
37	151835.0
38	180029.0
39	208223.0
40	208223.0
41	208223.0
42	234260.0
43	260297.0
44	260297.0
45	260297.0
46	283146.0
47	305995.0
48	305995.0
49	305995.0
50	309377.5
51	312760.0
52	312760.0
53	312760.0
54	296158.5
55	279557.0
56	279557.0
57	279557.0
58	247576.5
59	215596.0
60	215596.0
61	215596.0
62	182432.0
63	149268.0
64	149268.0
65	149268.0
66	117785.5
67	86303.0
68	86303.0
69	86303.0
70	64532.0
71	42761.0
72	42761.0
73	42761.0
74	30642.5
75	18524.0
76	18524.0
77	18524.0
78	13046.0
79	7568.0
80	7568.0
81	7568.0
82	5254.5
83	2941.0
84	2941.0
85	2941.0
86	2005.0
87	1069.0
88	1069.0
89	1069.0
90	693.0
91	317.0
92	317.0
93	317.0
94	180.5
95	44.0
96	44.0
97	44.0
98	29.5
99	15.0
100	15.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03373428394137985
2	0.05513749803473574
3	0.013916493114198477
4	0.01004101401910523
5	0.00898406517498889
6	7.927116330872551E-4
7	0.0031708465323490205
8	0.0016294628013460242
9	0.006782088416413183
10	0.003567202348892648
11	0.011318160539079143
12	0.007398641908814381
13	0.00854366982327375
14	0.015105560563829362
15	0.005945337248154413
16	0.03184058392900475
17	0.04003193747090638
18	0.02228400479678617
19	0.016735023365175385
20	0.015589995450716017
21	0.023164795500216454
22	0.006077455853668956
23	0.01594231173208813
24	0.01162643728527974
25	0.46994587981522773
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2270687.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.92437186823173
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.75083890511755	50.18271444069059
2	10.963131350539935	13.796963078832889
3	3.954453345768953	7.464944785942162
4	1.8417265624631098	4.635579483841154
5	1.0384435704403958	3.2671704695282875
6	0.6091200927181725	2.2997099535966044
7	0.3858995227789852	1.699773955258262
8	0.2852888348076305	1.43612965850319
9	0.19853641488317325	1.1243501279544896
>10	0.9187237654614664	9.875099709981372
>50	0.035920196145661686	1.547066508227643
>100	0.016486106611500247	1.7833958768767384
>500	0.0010019325844455417	0.43785432089464915
>1k	4.2939967904800366E-4	0.44924762987188493
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2665	0.11736536123208527	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.807907034302834E-5
2	4.403953517151417E-5	0.0	0.0	0.0	8.807907034302834E-5
3	4.403953517151417E-5	0.0	0.0	0.0	8.807907034302834E-5
4	4.403953517151417E-5	0.0	0.0	0.0	8.807907034302834E-5
5	4.403953517151417E-5	0.0	0.0	0.0	8.807907034302834E-5
6	4.403953517151417E-5	0.0	0.0	0.0	8.807907034302834E-5
7	4.403953517151417E-5	0.0	0.0	0.0	8.807907034302834E-5
8	4.403953517151417E-5	0.0	0.0	0.0	8.807907034302834E-5
9	4.403953517151417E-5	0.0	0.0	0.0	8.807907034302834E-5
10	4.403953517151417E-5	0.0	0.0	0.0	8.807907034302834E-5
11	4.403953517151417E-5	0.0	0.0	0.0	8.807907034302834E-5
12	8.807907034302834E-5	0.0	0.0	0.0	1.321186055145425E-4
13	8.807907034302834E-5	0.0	0.0	0.0	2.2019767585757086E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACCG	75	1.4858502E-5	12.66122	5
TCTTATA	130	1.9826984E-10	12.422385	2
GTTCGCG	55	0.003071994	12.089437	16
TTGGACT	295	0.0	11.588745	4
CGGCAAA	135	4.7439244E-9	11.257393	17
GTATTAG	170	1.382432E-10	10.618928	1
GGTATCA	1585	0.0	10.310391	1
TTAGACT	170	1.5697879E-9	10.054941	4
CTTAGAC	200	2.5465852E-11	9.971588	3
GTTATCA	220	1.8189894E-12	9.933016	1
CCGTCCA	115	1.0320686E-5	9.90878	9
TCTATAC	135	6.4756387E-7	9.848483	3
GTATCAA	2995	0.0	9.739071	1
GTCCTAA	210	6.730261E-11	9.501146	1
GTCTTGG	450	0.0	9.501145	1
TCACCGA	80	0.0045205187	9.499053	13
CTAGATC	90	0.0011174192	9.496751	3
ACCGTTC	100	2.7680252E-4	9.495914	8
GACGCTC	140	1.0651511E-6	9.495914	8
ACCGTCC	140	1.0651511E-6	9.495914	8
>>END_MODULE