FastQCFastQC Report
Mon 15 Aug 2016
SRR2921202_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921202_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2270687
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT49430.21768742235279454No Hit
GGTATCAACGCAGAGTACTTTTTTT32230.14193942185779018No Hit
TATCAACGCAGAGTACTTTTTTTTT30080.13247092179591463No Hit
CCCATGTACTCTGCGTTGATACCAC24150.10635547743920672No Hit
ACGCAGAGTACTTTTTTTTTTTTTT22790.10036610065588081No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGACG604.0771632E-412.67260554
CTTATAC3350.012.4969621
TATACCG853.950181E-612.2928345
GTCTTAC1302.5538611E-911.710441
TATACAC4250.011.4055963
CGTCTTA600.00589612211.07851515
TTACCGC600.005905795411.07607616
CCTACAC3500.010.8624733
AAGTGCG700.00150058610.8495567
GGTATCA19200.010.753631
AACCGCT1158.901188E-710.7316257
TTATACT1451.43372745E-810.4876734
CTACTAG1105.9372105E-610.3797071
TAGGACA2400.010.2964924
CTCTTAC2250.010.1490481
TTAGTAC1054.084388E-59.9572663
AATCGAA1054.1381365E-59.9454257
CTAAGGC2300.09.9179093
TTATACA4650.09.8112652
TCTTACA2550.09.691032
Produced by FastQC (version 0.11.5)