##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921202_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2270687
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.979464805144875	31.0	30.0	33.0	24.0	34.0
2	29.269634256064354	31.0	30.0	34.0	25.0	34.0
3	29.39863662407016	31.0	30.0	34.0	25.0	34.0
4	33.02891767997967	35.0	33.0	37.0	27.0	37.0
5	32.48301505227273	35.0	33.0	37.0	25.0	37.0
6	32.39353112075773	35.0	33.0	37.0	23.0	37.0
7	32.21364018907053	35.0	33.0	37.0	21.0	37.0
8	32.14132154717933	35.0	33.0	37.0	19.0	37.0
9	33.47514827010504	38.0	34.0	39.0	18.0	39.0
10	33.326414428760984	38.0	33.0	39.0	17.0	39.0
11	33.32886038454441	38.0	33.0	39.0	17.0	39.0
12	33.19536774553252	38.0	33.0	39.0	17.0	39.0
13	33.150902348055894	38.0	33.0	39.0	17.0	39.0
14	34.05217495850375	39.0	33.0	41.0	17.0	41.0
15	34.03432000975916	39.0	33.0	41.0	17.0	41.0
16	34.01304054675964	39.0	33.0	41.0	16.0	41.0
17	33.98026368231289	39.0	33.0	41.0	15.0	41.0
18	33.92916196728127	39.0	33.0	41.0	11.0	41.0
19	33.96318382938732	39.0	34.0	41.0	10.0	41.0
20	33.8962402127638	39.0	34.0	41.0	10.0	41.0
21	33.8126875258457	39.0	34.0	41.0	9.0	41.0
22	33.72503651978454	39.0	34.0	41.0	2.0	41.0
23	33.59225071531215	39.0	33.0	41.0	2.0	41.0
24	33.50459002055325	39.0	33.0	41.0	2.0	41.0
25	33.368152457824436	39.0	33.0	41.0	2.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	103500.0
3	9.0
4	1710.0
5	13991.0
6	15090.0
7	11469.0
8	8794.0
9	7329.0
10	6571.0
11	5982.0
12	5461.0
13	5065.0
14	4965.0
15	4776.0
16	4844.0
17	5135.0
18	5575.0
19	6575.0
20	8292.0
21	10380.0
22	13175.0
23	15944.0
24	18571.0
25	20988.0
26	23810.0
27	26120.0
28	30367.0
29	36301.0
30	45586.0
31	56862.0
32	72262.0
33	92670.0
34	120339.0
35	159213.0
36	215573.0
37	325031.0
38	762362.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.486088733229444	18.693886007621828	8.691174947156362	33.128850311992366
2	14.508252348298114	20.550124257548486	34.93973409809455	30.00188929605886
3	17.989605173710437	23.590360840661862	27.423194199621726	30.996839786005975
4	13.246549067420915	16.3323505734186	32.537446149060195	37.88365421010029
5	14.759316623059895	37.434397038882075	32.243142014408086	15.563144323649942
6	34.45381741369765	34.86298253626881	16.059620951896893	14.623579098136647
7	29.982700577293365	31.8480258046786	19.117667784141258	19.051605833886786
8	28.008317121757926	35.0828915431291	18.067718213237015	18.841073121875958
9	27.179883312076775	15.759387529330523	18.35883826461946	38.70189089397324
10	16.71511775132945	28.842480760126392	30.66682814581567	23.775573342728485
11	36.301322802823364	22.480556991863402	21.244387755551465	19.97373244976177
12	23.862356711488754	24.779922851338434	28.308027415563874	23.049693021608935
13	29.040157248313076	20.967748328708165	24.76189679236167	25.23019763061709
14	22.997339391893735	20.379605940937846	25.197871160999842	31.425183506168576
15	25.01536248188989	27.622337114743097	22.218047249047526	25.14425315431949
16	24.875678861024138	26.796418987452714	23.84825399873422	24.47964815278893
17	23.65535139953379	26.972024297898972	25.264803146589365	24.107821155977874
18	23.797873221298087	26.490700628414483	26.04726938316018	23.664156767127245
19	24.74582735815484	25.930233992100433	25.94062816009133	23.383310489653397
20	25.12793794855353	25.342679923475046	25.774982471840442	23.754399656130982
21	25.22524189140202	25.063944251448135	25.552153711101038	24.158660146048803
22	24.98152475594621	24.99676297869379	25.30932270944409	24.71238955591591
23	24.494920133255114	24.96890596565034	25.612499625638396	24.923674275456147
24	24.23646891040407	25.567533665310393	25.60690932762753	24.589088096658003
25	24.236586414919543	25.117730746749306	25.7182623272639	24.92742051106724
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	783.0
1	783.0
2	851.0
3	919.0
4	919.0
5	919.0
6	1204.5
7	1490.0
8	1490.0
9	1490.0
10	2013.0
11	2536.0
12	2536.0
13	2536.0
14	3665.5
15	4795.0
16	4795.0
17	4795.0
18	8921.0
19	13047.0
20	13047.0
21	13047.0
22	21110.5
23	29174.0
24	29174.0
25	29174.0
26	43814.0
27	58454.0
28	58454.0
29	58454.0
30	77088.5
31	95723.0
32	95723.0
33	95723.0
34	118562.0
35	141401.0
36	141401.0
37	141401.0
38	169681.5
39	197962.0
40	197962.0
41	197962.0
42	223510.0
43	249058.0
44	249058.0
45	249058.0
46	275384.5
47	301711.0
48	301711.0
49	301711.0
50	311650.5
51	321590.0
52	321590.0
53	321590.0
54	306672.5
55	291755.0
56	291755.0
57	291755.0
58	260235.5
59	228716.0
60	228716.0
61	228716.0
62	195361.5
63	162007.0
64	162007.0
65	162007.0
66	127534.5
67	93062.0
68	93062.0
69	93062.0
70	69253.5
71	45445.0
72	45445.0
73	45445.0
74	32297.0
75	19149.0
76	19149.0
77	19149.0
78	13506.5
79	7864.0
80	7864.0
81	7864.0
82	5376.5
83	2889.0
84	2889.0
85	2889.0
86	1879.0
87	869.0
88	869.0
89	869.0
90	555.5
91	242.0
92	242.0
93	242.0
94	142.0
95	42.0
96	42.0
97	42.0
98	23.0
99	4.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.09697505644767421
2	0.0
3	0.03981173979504881
4	0.05408054919061941
5	0.06799704230481789
6	0.009248302386017976
7	0.0040075977006077896
8	0.008367511682587692
9	6.605930275727126E-4
10	5.2847442205817E-4
11	0.009028104710160405
12	2.64237211029085E-4
13	0.006077455853668956
14	0.05641464455470966
15	0.024045586203646736
16	0.006870167486756211
17	0.0016735023365175386
18	0.00929234192118949
19	0.008059234936387095
20	0.0024221744344332793
21	0.01541383731002996
22	0.003567202348892648
23	0.006826127951584697
24	0.010965844257707028
25	0.17008068483238772
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2270687.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.56978271458049
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.37912361828863	36.09626771734036
2	14.062848567632654	14.223103928264655
3	5.766222021322461	8.74789784106918
4	2.895416636855692	5.85682360775895
5	1.6575872647446817	4.191191390429718
6	1.0076315608186532	3.0573425452171734
7	0.6575098635464902	2.3275091652567634
8	0.4617801697468455	1.8681698276800058
9	0.34378928525281865	1.5646814509352374
>10	1.6509669656870791	14.48696337440373
>50	0.0788148513921893	2.6741875416341836
>100	0.035068877943251545	3.0319876082925945
>500	0.0022769793509386563	0.791276866815322
>1k	9.633374177045516E-4	1.0825971349021417
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	4943	0.21768742235279454	No Hit
GGTATCAACGCAGAGTACTTTTTTT	3223	0.14193942185779018	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3008	0.13247092179591463	No Hit
CCCATGTACTCTGCGTTGATACCAC	2415	0.10635547743920672	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	2279	0.10036610065588081	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.7615814068605668E-4
2	0.0	0.0	0.0	0.0	1.7615814068605668E-4
3	0.0	0.0	0.0	0.0	2.2019767585757086E-4
4	0.0	0.0	0.0	0.0	2.2019767585757086E-4
5	0.0	0.0	0.0	0.0	2.64237211029085E-4
6	0.0	0.0	0.0	0.0	2.64237211029085E-4
7	0.0	0.0	0.0	0.0	2.64237211029085E-4
8	0.0	0.0	0.0	0.0	2.64237211029085E-4
9	0.0	0.0	0.0	0.0	2.64237211029085E-4
10	0.0	0.0	0.0	0.0	3.082767462005992E-4
11	0.0	0.0	0.0	0.0	3.5231628137211336E-4
12	0.0	0.0	0.0	0.0	6.165534924011984E-4
13	0.0	0.0	0.0	0.0	6.605930275727126E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGACG	60	4.0771632E-4	12.6726055	4
CTTATAC	335	0.0	12.496962	1
TATACCG	85	3.950181E-6	12.292834	5
GTCTTAC	130	2.5538611E-9	11.71044	1
TATACAC	425	0.0	11.405596	3
CGTCTTA	60	0.005896122	11.078515	15
TTACCGC	60	0.0059057954	11.076076	16
CCTACAC	350	0.0	10.862473	3
AAGTGCG	70	0.001500586	10.849556	7
GGTATCA	1920	0.0	10.75363	1
AACCGCT	115	8.901188E-7	10.731625	7
TTATACT	145	1.43372745E-8	10.487673	4
CTACTAG	110	5.9372105E-6	10.379707	1
TAGGACA	240	0.0	10.296492	4
CTCTTAC	225	0.0	10.149048	1
TTAGTAC	105	4.084388E-5	9.957266	3
AATCGAA	105	4.1381365E-5	9.945425	7
CTAAGGC	230	0.0	9.917909	3
TTATACA	465	0.0	9.811265	2
TCTTACA	255	0.0	9.69103	2
>>END_MODULE