FastQCFastQC Report
Mon 15 Aug 2016
SRR2921201_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921201_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3006553
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT34920.1161462977702372No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCT700.001496678310.853164
CTAAGCT1855.456968E-1210.7798284
GGTATCA18900.010.7583941
GTACTAA1451.4413672E-810.4844931
TCTATAC2350.010.1028443
GTATCAA37300.010.0365131
CTAACAC2650.010.034223
GACCGTG2002.5465852E-119.9710097
GGACCGT2107.2759576E-129.9480686
TACACCG1252.585719E-69.8758825
GTACTAG1502.644192E-79.5015721
ACCGTCC1304.258791E-69.4969898
TAACACT2201.6370905E-119.4965144
TGGACCG1804.2164174E-99.4960415
GTCTAGG2950.09.3405291
TAAAGGT3600.09.2327234
AATCCGG1554.2906504E-79.1895656
CGAGCAT2850.08.99834511
ACTCGTG950.00182837578.99789518
TAGCGCT850.0074605488.9378974
Produced by FastQC (version 0.11.5)