##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921201_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3006553
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.121769348486456	31.0	28.0	31.0	10.0	34.0
2	26.873422487479846	31.0	27.0	31.0	10.0	34.0
3	26.63881062465887	31.0	26.0	31.0	10.0	34.0
4	30.490828533539904	35.0	30.0	37.0	10.0	37.0
5	30.26902735458181	35.0	30.0	37.0	10.0	37.0
6	30.797128139766702	35.0	32.0	37.0	10.0	37.0
7	30.774789601247676	35.0	32.0	37.0	10.0	37.0
8	30.40892344156248	35.0	31.0	37.0	8.0	37.0
9	32.05596575214207	37.0	32.0	39.0	8.0	39.0
10	31.40582786998932	37.0	30.0	39.0	8.0	39.0
11	31.865158206091827	37.0	32.0	39.0	2.0	39.0
12	31.542017054081533	37.0	31.0	39.0	2.0	39.0
13	31.554980404469838	37.0	31.0	39.0	2.0	39.0
14	32.66985581162215	38.0	32.0	40.0	2.0	41.0
15	32.31803829834365	38.0	31.0	40.0	2.0	41.0
16	32.243611870470936	38.0	31.0	40.0	2.0	41.0
17	32.56328559649539	38.0	32.0	40.0	2.0	41.0
18	32.61664570689424	38.0	32.0	40.0	2.0	41.0
19	32.30927144806694	38.0	31.0	40.0	2.0	41.0
20	32.19578666998387	38.0	31.0	40.0	2.0	41.0
21	32.135625415550635	38.0	31.0	40.0	2.0	41.0
22	31.372524282791623	38.0	30.0	40.0	2.0	41.0
23	31.491581222749108	38.0	30.0	40.0	2.0	41.0
24	31.138146575164317	38.0	30.0	40.0	2.0	41.0
25	30.63967174368787	38.0	30.0	40.0	2.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	240717.0
3	10.0
4	1737.0
5	10169.0
6	12534.0
7	11234.0
8	9711.0
9	9012.0
10	8626.0
11	8250.0
12	7838.0
13	7855.0
14	7669.0
15	7886.0
16	8511.0
17	9322.0
18	10787.0
19	13421.0
20	16592.0
21	20875.0
22	25131.0
23	29747.0
24	34425.0
25	38906.0
26	43848.0
27	48888.0
28	55086.0
29	64393.0
30	76584.0
31	90976.0
32	114103.0
33	144306.0
34	186664.0
35	242517.0
36	324282.0
37	441165.0
38	622776.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.31028011884838	17.46206134732541	11.788382005050725	25.439276528775483
2	16.30377160390089	19.137591078734957	38.57338961814736	25.985247699216796
3	19.607694418801927	22.75585273155194	29.226649368362402	28.409803481283735
4	13.826388593204461	15.372389402197054	34.130306352720766	36.67091565187771
5	14.361907904562859	36.40028726809571	34.023273505771805	15.214531321569622
6	35.13092808467951	33.45298376603507	16.661300579803466	14.754787569481955
7	30.167922579658036	30.928621677762276	19.891233666340145	19.012222076239546
8	27.011317815173065	33.84531310338038	19.931920815539936	19.211448265906625
9	26.80527762550759	15.55300244282772	18.748370117670852	38.89334981399384
10	16.019520651717286	27.86319578161271	32.30293997219984	23.81434359447017
11	36.21539557724683	22.184385942911163	21.47309567773824	20.127122802103766
12	23.742691186713778	24.256910982329785	28.69159518903956	23.308802641916877
13	28.944808899125107	20.863128917167113	24.833134751546577	25.358927432161206
14	23.056590115857446	20.25948502182889	25.33345442013394	31.350470442179724
15	24.896594598604096	27.56881968326597	22.547150700961392	24.98743501716854
16	24.574331886713857	26.75793544643582	24.427407516831757	24.240325150018567
17	23.76323377625044	26.759628905249187	25.29378999912823	24.18334731937214
18	23.753010370745933	26.593961992229175	25.8526431213174	23.80038451570749
19	24.77032998763827	25.97203907317638	26.190897562650278	23.06673337653507
20	25.28066564029822	25.305482111515776	25.73657681729089	23.677275430895108
21	25.52669881514183	24.975506055701473	25.47533222770968	24.022462901447014
22	24.372409466670703	24.46444709732206	26.762261178647133	24.4008822573601
23	24.90674797686438	24.936620559322524	25.577018203979172	24.57961325983392
24	24.555851240769076	25.185017630230856	25.76029538952831	24.498835739471758
25	24.02954626336006	24.989248461619432	26.485040575153	24.496164699867506
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1156.0
1	1156.0
2	1233.0
3	1310.0
4	1310.0
5	1310.0
6	1840.5
7	2371.0
8	2371.0
9	2371.0
10	3120.5
11	3870.0
12	3870.0
13	3870.0
14	5924.0
15	7978.0
16	7978.0
17	7978.0
18	14076.0
19	20174.0
20	20174.0
21	20174.0
22	30596.5
23	41019.0
24	41019.0
25	41019.0
26	60102.5
27	79186.0
28	79186.0
29	79186.0
30	106128.0
31	133070.0
32	133070.0
33	133070.0
34	162952.5
35	192835.0
36	192835.0
37	192835.0
38	231109.5
39	269384.0
40	269384.0
41	269384.0
42	305146.5
43	340909.0
44	340909.0
45	340909.0
46	370869.0
47	400829.0
48	400829.0
49	400829.0
50	409403.5
51	417978.0
52	417978.0
53	417978.0
54	396177.0
55	374376.0
56	374376.0
57	374376.0
58	334593.5
59	294811.0
60	294811.0
61	294811.0
62	251051.5
63	207292.0
64	207292.0
65	207292.0
66	163792.0
67	120292.0
68	120292.0
69	120292.0
70	89761.5
71	59231.0
72	59231.0
73	59231.0
74	41639.0
75	24047.0
76	24047.0
77	24047.0
78	16760.0
79	9473.0
80	9473.0
81	9473.0
82	6483.0
83	3493.0
84	3493.0
85	3493.0
86	2307.5
87	1122.0
88	1122.0
89	1122.0
90	711.0
91	300.0
92	300.0
93	300.0
94	166.5
95	33.0
96	33.0
97	33.0
98	23.5
99	14.0
100	14.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03469089019884233
2	0.05315056810906044
3	0.015898605479431095
4	0.01147493491716261
5	0.010044725637632198
6	5.654315756283026E-4
7	0.003592153539285687
8	0.0017960767696428436
9	0.006685396864781695
10	0.003193025368253944
11	0.010842981979695684
12	0.00834843091074729
13	0.007816260016038301
14	0.014801003009093802
15	0.006053443927314769
16	0.03076612985036352
17	0.0390813000801915
18	0.02308291255800247
19	0.01666360114057527
20	0.01613143024586628
21	0.023548562090872836
22	0.005787358479960273
23	0.015033827775528988
24	0.011741020364517107
25	0.4642525842717557
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3006553.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.717557460627376
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.39164866270936	29.215241815763264
2	12.336393009267342	10.53961154460519
3	6.148326064423135	7.879244158310048
4	3.5453847286415554	6.058007034631058
5	2.252039268464586	4.810080842711259
6	1.581339726309374	4.053058237403607
7	1.1297177505876639	3.378114804751347
8	0.8371888646380372	2.8610130744458195
9	0.6326884325280868	2.4324214024073534
>10	2.981258753712092	21.322810624341347
>50	0.1201932339868352	3.483723537066548
>100	0.04162699239395995	3.002288075096066
>500	0.0014630082253982496	0.45274979925309394
>1k	7.315041126929636E-4	0.5116350492138987
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3492	0.1161462977702372	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.328247664351834E-4
2	0.0	3.326068091931192E-5	0.0	0.0	2.328247664351834E-4
3	0.0	3.326068091931192E-5	0.0	0.0	2.328247664351834E-4
4	0.0	3.326068091931192E-5	0.0	0.0	2.328247664351834E-4
5	0.0	3.326068091931192E-5	0.0	0.0	2.6608544735449535E-4
6	3.326068091931192E-5	3.326068091931192E-5	0.0	0.0	3.326068091931192E-4
7	3.326068091931192E-5	3.326068091931192E-5	0.0	0.0	3.326068091931192E-4
8	3.326068091931192E-5	3.326068091931192E-5	0.0	0.0	3.326068091931192E-4
9	3.326068091931192E-5	3.326068091931192E-5	0.0	0.0	3.658674901124311E-4
10	3.326068091931192E-5	3.326068091931192E-5	0.0	0.0	3.9912817103174297E-4
11	3.326068091931192E-5	3.326068091931192E-5	0.0	0.0	3.9912817103174297E-4
12	3.326068091931192E-5	3.326068091931192E-5	0.0	0.0	9.312990657407336E-4
13	3.326068091931192E-5	3.326068091931192E-5	0.0	0.0	0.0010976024703372932
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAACGCT	70	0.0014966783	10.85316	4
CTAAGCT	185	5.456968E-12	10.779828	4
GGTATCA	1890	0.0	10.758394	1
GTACTAA	145	1.4413672E-8	10.484493	1
TCTATAC	235	0.0	10.102844	3
GTATCAA	3730	0.0	10.036513	1
CTAACAC	265	0.0	10.03422	3
GACCGTG	200	2.5465852E-11	9.971009	7
GGACCGT	210	7.2759576E-12	9.948068	6
TACACCG	125	2.585719E-6	9.875882	5
GTACTAG	150	2.644192E-7	9.501572	1
ACCGTCC	130	4.258791E-6	9.496989	8
TAACACT	220	1.6370905E-11	9.496514	4
TGGACCG	180	4.2164174E-9	9.496041	5
GTCTAGG	295	0.0	9.340529	1
TAAAGGT	360	0.0	9.232723	4
AATCCGG	155	4.2906504E-7	9.189565	6
CGAGCAT	285	0.0	8.998345	11
ACTCGTG	95	0.0018283757	8.997895	18
TAGCGCT	85	0.007460548	8.937897	4
>>END_MODULE