FastQCFastQC Report
Mon 15 Aug 2016
SRR2921201_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921201_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3006553
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT60610.2015929870519495No Hit
GGTATCAACGCAGAGTACTTTTTTT39970.13294294163448975No Hit
TATCAACGCAGAGTACTTTTTTTTT36630.12183387420743955No Hit
ACGCAGAGTACTTTTTTTTTTTTTT30400.10111246999470823No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGGT400.005261196314.2579184
TATCGTC551.96184E-413.8175755
CGTGCGG550.00306945212.09098210
GGTATCA22200.011.8720511
CTAAGCT1658.0035534E-1110.9454724
GTCCTAG2900.010.4990721
GTCACGC1002.4055511E-510.4479778
GACCGTG2300.010.3187677
TACGCTG750.002649764810.1328895
GTATTAC1356.3126936E-79.8671851
CTAGCCA3550.09.6391564
TAGACAG3950.09.6198325
GCCTTAG2201.6370905E-119.5147851
CCCCTAA1106.725007E-59.5147851
GAACTAA1401.0371932E-69.5147851
TCCTATA1804.145477E-99.5054372
TATACAC3700.09.2485333
GTATCAA40200.09.2070931
CATTCCG1451.7085422E-69.1720115
TAAAGGT2501.8189894E-129.1250674
Produced by FastQC (version 0.11.5)