##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921201_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3006553
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.528810568115713	31.0	30.0	33.0	26.0	34.0
2	29.78920278471725	31.0	30.0	34.0	26.0	34.0
3	29.90716445045206	31.0	30.0	34.0	26.0	34.0
4	33.57380994115188	35.0	35.0	37.0	28.0	37.0
5	33.09965166088873	35.0	35.0	37.0	27.0	37.0
6	33.03504844251872	36.0	35.0	37.0	26.0	37.0
7	32.87927536950122	36.0	33.0	37.0	26.0	37.0
8	32.81301277576015	36.0	33.0	37.0	25.0	37.0
9	34.20911788350313	38.0	35.0	39.0	25.0	39.0
10	34.08529768143119	38.0	34.0	39.0	25.0	39.0
11	34.08313673499187	38.0	35.0	39.0	25.0	39.0
12	33.958068259565025	38.0	34.0	39.0	25.0	39.0
13	33.93103298029338	38.0	34.0	39.0	25.0	39.0
14	34.87545538029764	39.0	34.0	41.0	24.0	41.0
15	34.8610797813975	39.0	34.0	41.0	24.0	41.0
16	34.8438108358642	39.0	34.0	41.0	24.0	41.0
17	34.81970116608621	39.0	34.0	41.0	23.0	41.0
18	34.77090408850268	39.0	34.0	41.0	23.0	41.0
19	34.803965870550094	39.0	34.0	41.0	23.0	41.0
20	34.73861162600493	39.0	34.0	41.0	22.0	41.0
21	34.66693119994891	39.0	34.0	41.0	21.0	41.0
22	34.58224817590111	39.0	34.0	41.0	19.0	41.0
23	34.454237128033334	39.0	34.0	41.0	18.0	41.0
24	34.37697755535991	39.0	34.0	41.0	18.0	41.0
25	34.23381793036744	39.0	34.0	41.0	17.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	103197.0
3	4.0
4	1582.0
5	14101.0
6	15753.0
7	11885.0
8	9279.0
9	8037.0
10	7075.0
11	6699.0
12	6159.0
13	5685.0
14	5623.0
15	5567.0
16	5598.0
17	5805.0
18	6488.0
19	7603.0
20	9451.0
21	12496.0
22	15517.0
23	19058.0
24	22685.0
25	26151.0
26	29972.0
27	33135.0
28	38307.0
29	46371.0
30	58582.0
31	74776.0
32	95052.0
33	122689.0
34	160983.0
35	212812.0
36	290096.0
37	437842.0
38	1074438.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.57508604182946	18.366355572084526	8.769857375491972	32.28870101059404
2	14.887015129951141	20.29975856071721	34.575641939456915	30.23758436987474
3	18.220851057033492	23.13375426566651	27.36614724468555	31.279247432614447
4	13.464618026436645	15.764446308054792	32.140522875817	38.63041278969157
5	14.818458605526718	37.18020744164622	32.3739501112293	15.62738384159776
6	35.285342369931755	34.266263665244054	15.708740001882623	14.739653962941565
7	30.531116945112164	31.35196821034867	18.943872852038226	19.17304199250095
8	28.299281714571766	34.66675979929918	17.928929822898322	19.10502866323074
9	26.888912984476526	15.412214156852519	18.409476129403064	39.28939672926789
10	16.9034500054215	28.464590264043853	30.459138160533715	24.172821570000924
11	36.43116939272736	22.20001829384911	21.07491331210138	20.293899001322146
12	23.947440106620512	24.42626118765323	28.020978190624408	23.605320515101855
13	29.06720709609652	20.60639987121229	24.626625396058806	25.699767636632387
14	23.09490887490079	20.096441353972715	24.97345200478546	31.835197766341032
15	24.92571227061626	27.569297510213314	22.101780510200008	25.40320970897042
16	25.034624933144944	26.579209606905565	23.663297970001036	24.72286748994846
17	23.745482339464647	26.72915057671059	25.2014445870228	24.323922496801963
18	23.884712521374755	26.362338252839628	25.80816619640262	23.944783029382993
19	24.915457133515364	25.868470691307362	25.7232502112948	23.492821963882477
20	25.210641924029208	25.188090964848442	25.609141985894674	23.992125125227673
21	25.313740308710887	24.771014812853505	25.525461795664317	24.389783082771295
22	25.10081449866157	24.542260267047	25.320637336554785	25.03628789773665
23	24.888982020592085	24.769341043307904	25.430942010547163	24.910734925552845
24	24.689884242520602	25.17886496335555	25.374643517388236	24.756607276735615
25	24.523263069766386	24.958297283151705	25.588560512499487	24.92987913458242
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1020.0
1	1020.0
2	1094.5
3	1169.0
4	1169.0
5	1169.0
6	1537.0
7	1905.0
8	1905.0
9	1905.0
10	2581.0
11	3257.0
12	3257.0
13	3257.0
14	4601.5
15	5946.0
16	5946.0
17	5946.0
18	11220.5
19	16495.0
20	16495.0
21	16495.0
22	26452.5
23	36410.0
24	36410.0
25	36410.0
26	53411.5
27	70413.0
28	70413.0
29	70413.0
30	94997.5
31	119582.0
32	119582.0
33	119582.0
34	147379.5
35	175177.0
36	175177.0
37	175177.0
38	213922.5
39	252668.0
40	252668.0
41	252668.0
42	287860.5
43	323053.0
44	323053.0
45	323053.0
46	359414.5
47	395776.0
48	395776.0
49	395776.0
50	411573.0
51	427370.0
52	427370.0
53	427370.0
54	410118.5
55	392867.0
56	392867.0
57	392867.0
58	353529.5
59	314192.0
60	314192.0
61	314192.0
62	270932.0
63	227672.0
64	227672.0
65	227672.0
66	180220.5
67	132769.0
68	132769.0
69	132769.0
70	99237.5
71	65706.0
72	65706.0
73	65706.0
74	46155.5
75	26605.0
76	26605.0
77	26605.0
78	18699.0
79	10793.0
80	10793.0
81	10793.0
82	7377.0
83	3961.0
84	3961.0
85	3961.0
86	2651.0
87	1341.0
88	1341.0
89	1341.0
90	848.5
91	356.0
92	356.0
93	356.0
94	200.0
95	44.0
96	44.0
97	44.0
98	25.0
99	6.0
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.10217681178412621
2	0.0
3	0.038615650547321134
4	0.054314691941236365
5	0.06482506711173892
6	0.003725196262962935
7	0.0013304272367724768
8	0.0029269399208994486
9	2.9934612827380724E-4
10	2.3282476643518343E-4
11	0.0025943331117063294
12	6.652136183862382E-5
13	0.0018293374505621554
14	0.05381578172744668
15	0.022384438258696918
16	0.001629773365046284
17	6.319529374669264E-4
18	0.002860418559060825
19	0.0029934612827380723
20	9.645597466600456E-4
21	0.008148866825231419
22	0.0013636879176917886
23	0.0019291194933200911
24	0.003159764687334632
25	0.16464037055059397
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3006553.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.543981210503848
#Duplication Level	Percentage of deduplicated	Percentage of total
1	59.019825919201175	18.617202798424895
2	13.745415399738329	8.671702501998322
3	7.549134563947447	7.143892765221703
4	4.702930835187738	5.933966475978447
5	3.2511069501995067	5.127642827521586
6	2.2599099719156115	4.277193461492179
7	1.6299667560475146	3.5990948508556095
8	1.249449973769843	3.1530101196848364
9	1.0166431785110843	2.88620759886665
>10	5.243811361217355	28.629678350833217
>50	0.23212460039362556	5.006548920129009
>100	0.09467518897588789	5.138056700111454
>500	0.003407864439109624	0.7219472424804865
>1k	0.0014909406920709592	0.8902481909905129
>5k	1.0649576371935423E-4	0.2036071954112486
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	6061	0.2015929870519495	No Hit
GGTATCAACGCAGAGTACTTTTTTT	3997	0.13294294163448975	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3663	0.12183387420743955	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	3040	0.10111246999470823	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.658674901124311E-4
2	0.0	0.0	0.0	0.0	3.658674901124311E-4
3	0.0	0.0	0.0	0.0	3.9912817103174297E-4
4	0.0	0.0	0.0	0.0	3.9912817103174297E-4
5	0.0	0.0	0.0	0.0	4.656495328703668E-4
6	0.0	0.0	0.0	0.0	4.989102137896788E-4
7	0.0	0.0	0.0	0.0	4.989102137896788E-4
8	0.0	0.0	0.0	0.0	4.989102137896788E-4
9	0.0	0.0	0.0	3.326068091931192E-5	4.989102137896788E-4
10	0.0	0.0	0.0	3.326068091931192E-5	5.321708947089907E-4
11	0.0	0.0	0.0	3.326068091931192E-5	5.321708947089907E-4
12	0.0	0.0	0.0	3.326068091931192E-5	0.0012639058749338529
13	0.0	0.0	0.0	3.326068091931192E-5	0.0018293374505621554
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGGGT	40	0.0052611963	14.257918	4
TATCGTC	55	1.96184E-4	13.817575	5
CGTGCGG	55	0.003069452	12.090982	10
GGTATCA	2220	0.0	11.872051	1
CTAAGCT	165	8.0035534E-11	10.945472	4
GTCCTAG	290	0.0	10.499072	1
GTCACGC	100	2.4055511E-5	10.447977	8
GACCGTG	230	0.0	10.318767	7
TACGCTG	75	0.0026497648	10.132889	5
GTATTAC	135	6.3126936E-7	9.867185	1
CTAGCCA	355	0.0	9.639156	4
TAGACAG	395	0.0	9.619832	5
GCCTTAG	220	1.6370905E-11	9.514785	1
CCCCTAA	110	6.725007E-5	9.514785	1
GAACTAA	140	1.0371932E-6	9.514785	1
TCCTATA	180	4.145477E-9	9.505437	2
TATACAC	370	0.0	9.248533	3
GTATCAA	4020	0.0	9.207093	1
CATTCCG	145	1.7085422E-6	9.172011	5
TAAAGGT	250	1.8189894E-12	9.125067	4
>>END_MODULE