FastQCFastQC Report
Mon 15 Aug 2016
SRR2921200_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921200_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2991085
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11500.012.2290151
GTATCAA27450.011.7004341
GGACCGT1900.011.4954466
GACCGTC1251.844819E-811.3954857
GTATTAA3500.011.1312361
GTATACG1158.797797E-710.7417031
CCGTCCG909.5592055E-510.5513769
CTTACGC856.619785E-410.0550083
TCTACAC4700.09.9004993
CTATACT2800.09.8352254
CTCTATA1856.493792E-109.7566452
GTATTAG2750.09.6750441
GTCTAAG3550.09.636111
GTATAAA4450.09.3955091
TATACAG4550.09.1831765
TCTATAC3350.09.0711853
CGCCCTA1054.5018183E-49.04615410
TATGCCG1054.512364E-49.0440375
TACGGTT1054.5131182E-49.0438854
GTCTAAC2002.6466296E-99.0271621
Produced by FastQC (version 0.11.5)