##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921200_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2991085
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.597982337512978	31.0	27.0	31.0	7.0	34.0
2	26.406312425089894	31.0	26.0	31.0	7.0	34.0
3	26.244972978033054	31.0	26.0	31.0	7.0	34.0
4	30.03186602854817	35.0	30.0	37.0	7.0	37.0
5	29.762088004854427	35.0	30.0	37.0	7.0	37.0
6	30.05534613693693	35.0	30.0	37.0	2.0	37.0
7	29.960719605093136	35.0	30.0	37.0	2.0	37.0
8	29.62877818584226	35.0	30.0	37.0	2.0	37.0
9	31.219855336775787	37.0	30.0	39.0	2.0	39.0
10	30.555508786945204	37.0	30.0	39.0	2.0	39.0
11	30.992662528814794	37.0	30.0	39.0	2.0	39.0
12	30.682895337310708	37.0	30.0	39.0	2.0	39.0
13	30.687456224079224	37.0	30.0	39.0	2.0	39.0
14	31.749947928594473	38.0	31.0	40.0	2.0	41.0
15	31.397847603795945	38.0	30.0	40.0	2.0	41.0
16	31.314586512920897	38.0	30.0	40.0	2.0	41.0
17	31.615697313850994	38.0	30.0	40.0	2.0	41.0
18	31.66140113035905	38.0	31.0	40.0	2.0	41.0
19	31.36870533602355	38.0	30.0	40.0	2.0	41.0
20	31.232148200402197	38.0	30.0	40.0	2.0	41.0
21	31.176219331780942	38.0	30.0	40.0	2.0	41.0
22	30.430720624790002	37.0	27.0	40.0	2.0	41.0
23	30.515896071158124	37.0	29.0	40.0	2.0	41.0
24	30.15525102095059	37.0	27.0	40.0	2.0	41.0
25	29.664408734623056	37.0	27.0	40.0	2.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	283019.0
3	19.0
4	2394.0
5	13237.0
6	15311.0
7	13307.0
8	11265.0
9	10423.0
10	9808.0
11	9293.0
12	9026.0
13	8701.0
14	8518.0
15	8806.0
16	9297.0
17	10185.0
18	11660.0
19	14233.0
20	17908.0
21	22345.0
22	27023.0
23	32049.0
24	36335.0
25	41630.0
26	46156.0
27	51293.0
28	56492.0
29	66582.0
30	77986.0
31	92195.0
32	115007.0
33	144108.0
34	184186.0
35	235890.0
36	309647.0
37	407667.0
38	578084.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.4450475047983	17.216706090961598	11.820365677753962	25.517880726486137
2	15.698529731508918	18.83321146323727	39.52360945997387	25.944649345279945
3	19.32605361384005	22.560532159376304	29.228641082990077	28.884773143793574
4	13.399603449255881	14.864066016002461	35.29943593498708	36.43689459975458
5	14.155998480686257	36.24021339938425	34.617550213586625	14.986237906342867
6	35.00298221237364	34.025338106656214	16.49290487544917	14.478774805520972
7	30.244641733528677	30.823447518644354	19.86776267090518	19.064148076921793
8	26.992169568739527	34.317857134498794	20.065475925795287	18.624497370966388
9	26.777437451540848	15.532305935195211	18.62091977688478	39.069336836379165
10	15.734274735631807	27.78683643826026	32.82759372370235	23.651295102405584
11	36.590903163637265	22.21453939717761	21.52709745024572	19.667459988939406
12	23.692623940179978	24.001166228327982	29.228029067438626	23.078180764053418
13	29.106958546161664	20.705554138278874	25.05947268168549	25.128014633873967
14	22.600855326732805	20.02624829887617	25.561028130820183	31.81186824357084
15	24.666521336749923	27.63409359603276	22.61586494481921	25.083520122398106
16	24.465308688629303	26.829942847205736	24.479621968878646	24.225126495286318
17	23.747329005338145	26.872890252846915	25.375661665871235	24.004119075943706
18	23.657793079832853	26.713020925292795	25.970255961648558	23.658930033225793
19	24.742667576632947	26.02733175794585	26.18800255601994	23.041998109401266
20	24.964062719958054	25.516187742570374	25.953821723671993	23.565927813799583
21	25.349447371553847	25.28340349032716	25.552059967175357	23.815089170943633
22	24.25927721477935	24.66189446092018	26.782873554379407	24.29595476992106
23	24.498087714358892	25.134000432011373	25.952381111606726	24.415530742023
24	24.359813693463554	25.32184019499995	25.90245990163068	24.41588620990581
25	24.101629321813984	25.106180164185016	26.442244876229783	24.34994563777122
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	789.0
1	789.0
2	848.5
3	908.0
4	908.0
5	908.0
6	1282.0
7	1656.0
8	1656.0
9	1656.0
10	2286.5
11	2917.0
12	2917.0
13	2917.0
14	5087.0
15	7257.0
16	7257.0
17	7257.0
18	13209.0
19	19161.0
20	19161.0
21	19161.0
22	30911.5
23	42662.0
24	42662.0
25	42662.0
26	62731.5
27	82801.0
28	82801.0
29	82801.0
30	109403.0
31	136005.0
32	136005.0
33	136005.0
34	168137.5
35	200270.0
36	200270.0
37	200270.0
38	238875.5
39	277481.0
40	277481.0
41	277481.0
42	310357.0
43	343233.0
44	343233.0
45	343233.0
46	371299.5
47	399366.0
48	399366.0
49	399366.0
50	406225.0
51	413084.0
52	413084.0
53	413084.0
54	392221.5
55	371359.0
56	371359.0
57	371359.0
58	329492.0
59	287625.0
60	287625.0
61	287625.0
62	242119.5
63	196614.0
64	196614.0
65	196614.0
66	155124.5
67	113635.0
68	113635.0
69	113635.0
70	85360.5
71	57086.0
72	57086.0
73	57086.0
74	40143.0
75	23200.0
76	23200.0
77	23200.0
78	16274.5
79	9349.0
80	9349.0
81	9349.0
82	6308.5
83	3268.0
84	3268.0
85	3268.0
86	2166.5
87	1065.0
88	1065.0
89	1065.0
90	653.0
91	241.0
92	241.0
93	241.0
94	139.0
95	37.0
96	37.0
97	37.0
98	26.5
99	16.0
100	16.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.032295972866033563
2	0.052255285289451815
3	0.013439939018784153
4	0.009862641817266978
5	0.0087259305569718
6	5.683556301475886E-4
7	0.003309835728506545
8	0.002072826415832382
9	0.005482960196717913
10	0.0028752108348642715
11	0.00959518034425635
12	0.008257872979203199
13	0.007221459771287008
14	0.013874563912426427
15	0.006318777299876132
16	0.028584944928011075
17	0.03657535643420364
18	0.02129661978847141
19	0.016649476694911715
20	0.015312169329858563
21	0.021697811997987353
22	0.005182066039580955
23	0.014142025385437056
24	0.010531295499793553
25	0.4625077522036318
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2991085.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.18727267067547
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.5262823589801	39.106624282881505
2	12.283752445990627	13.066785815279605
3	5.442389205453888	8.683975159512505
4	2.826009448472486	6.012309404232452
5	1.6376396481337807	4.355079325080022
6	1.0404272406331967	3.320249240493379
7	0.7126703819608362	2.6533495750765805
8	0.504268030055737	2.1456512970943082
9	0.3558754230127152	1.7035238844512337
>10	1.5834073571815888	14.054509369555756
>50	0.06174158049548538	2.2137462639249628
>100	0.024371217320918726	2.111813385037517
>500	8.418672229214071E-4	0.31150751166254426
>1k	3.2379508564718795E-4	0.2608754857175673
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.3373073650531497E-4
2	0.0	0.0	0.0	0.0	1.3373073650531497E-4
3	0.0	0.0	0.0	0.0	1.3373073650531497E-4
4	0.0	0.0	0.0	0.0	1.3373073650531497E-4
5	0.0	0.0	0.0	0.0	1.3373073650531497E-4
6	0.0	0.0	0.0	0.0	1.671634206316437E-4
7	0.0	0.0	0.0	0.0	1.671634206316437E-4
8	0.0	0.0	0.0	0.0	2.3402878888430118E-4
9	0.0	0.0	0.0	3.343268412632874E-5	2.3402878888430118E-4
10	0.0	0.0	0.0	3.343268412632874E-5	2.3402878888430118E-4
11	0.0	0.0	0.0	3.343268412632874E-5	2.3402878888430118E-4
12	0.0	0.0	0.0	3.343268412632874E-5	4.346248936422736E-4
13	0.0	0.0	0.0	3.343268412632874E-5	4.6805757776860235E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1150	0.0	12.229015	1
GTATCAA	2745	0.0	11.700434	1
GGACCGT	190	0.0	11.495446	6
GACCGTC	125	1.844819E-8	11.395485	7
GTATTAA	350	0.0	11.131236	1
GTATACG	115	8.797797E-7	10.741703	1
CCGTCCG	90	9.5592055E-5	10.551376	9
CTTACGC	85	6.619785E-4	10.055008	3
TCTACAC	470	0.0	9.900499	3
CTATACT	280	0.0	9.835225	4
CTCTATA	185	6.493792E-10	9.756645	2
GTATTAG	275	0.0	9.675044	1
GTCTAAG	355	0.0	9.63611	1
GTATAAA	445	0.0	9.395509	1
TATACAG	455	0.0	9.183176	5
TCTATAC	335	0.0	9.071185	3
CGCCCTA	105	4.5018183E-4	9.046154	10
TATGCCG	105	4.512364E-4	9.044037	5
TACGGTT	105	4.5131182E-4	9.043885	4
GTCTAAC	200	2.6466296E-9	9.027162	1
>>END_MODULE