FastQCFastQC Report
Mon 15 Aug 2016
SRR2921200_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921200_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2991085
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT45250.15128289567163755No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15450.012.4366011
ACGATCG658.03921E-411.6902295
GTATCAA30050.010.0661051
CTTAGAC2650.010.0394773
TATACCG1054.115692E-59.950615
CCCTTAA5000.09.8926391
AGGACCG2600.09.863635
ACCGTCC1451.6220656E-79.8261678
ACACCGT1856.5847416E-109.749646
GTCTTAA2550.09.6986661
TTAGACA2950.09.6625324
GTCTACG1002.721578E-49.5121531
GTCTTAG2600.09.5121531
GTACCGA900.0011216079.4930716
GACCGTC1804.2400643E-99.4930717
CTAAACT2151.05501385E-109.2805244
CTATTAG1752.617162E-89.2403761
CTTATAC3450.09.0985811
CTTAGGC2950.09.0185143
GGACCGT1804.2726242E-88.9656776
Produced by FastQC (version 0.11.5)