##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921200_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2991085
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.497461289130868	31.0	30.0	33.0	26.0	34.0
2	29.775883667632314	31.0	30.0	34.0	26.0	34.0
3	29.89718881275524	31.0	30.0	34.0	26.0	34.0
4	33.56289339821503	35.0	35.0	37.0	28.0	37.0
5	33.079396272590046	35.0	35.0	37.0	26.0	37.0
6	33.01147643748004	36.0	35.0	37.0	26.0	37.0
7	32.84774454754713	36.0	33.0	37.0	26.0	37.0
8	32.790749510629084	36.0	33.0	37.0	25.0	37.0
9	34.190553595100106	38.0	35.0	39.0	25.0	39.0
10	34.05715484514816	38.0	34.0	39.0	25.0	39.0
11	34.0593289725969	38.0	34.0	39.0	25.0	39.0
12	33.92789138389581	38.0	34.0	39.0	25.0	39.0
13	33.90264001190204	38.0	34.0	39.0	25.0	39.0
14	34.84361527673069	39.0	34.0	41.0	24.0	41.0
15	34.837716748270275	39.0	34.0	41.0	24.0	41.0
16	34.823528920107584	39.0	34.0	41.0	24.0	41.0
17	34.79162578128004	39.0	34.0	41.0	23.0	41.0
18	34.7368386388217	39.0	34.0	41.0	23.0	41.0
19	34.77386500216477	39.0	34.0	41.0	22.0	41.0
20	34.70867929196262	39.0	34.0	41.0	22.0	41.0
21	34.643046921100535	39.0	34.0	41.0	21.0	41.0
22	34.55699353244726	39.0	34.0	41.0	19.0	41.0
23	34.42844586496204	39.0	34.0	41.0	18.0	41.0
24	34.35267235802393	39.0	34.0	41.0	18.0	41.0
25	34.21257436682675	39.0	34.0	41.0	16.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	102851.0
3	6.0
4	1649.0
5	14267.0
6	15709.0
7	11925.0
8	9117.0
9	7930.0
10	7031.0
11	6489.0
12	6066.0
13	5729.0
14	5541.0
15	5626.0
16	5685.0
17	5865.0
18	6492.0
19	7560.0
20	9773.0
21	12452.0
22	15651.0
23	19268.0
24	22745.0
25	26210.0
26	30065.0
27	33538.0
28	38635.0
29	46446.0
30	58971.0
31	74558.0
32	94137.0
33	122513.0
34	160551.0
35	212425.0
36	289583.0
37	438055.0
38	1059971.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.98206930524749	18.53444179250726	8.580864823042315	32.90262407920294
2	14.583169652483965	20.25910330197905	34.99315465792513	30.164572387611855
3	17.987300429952523	22.9165196421912	27.55375242694904	31.542427500907234
4	13.039067057734199	15.663151162230585	33.02134458009302	38.2764371999422
5	14.573835227016103	37.22342088737503	32.79143036755434	15.411313518054529
6	35.21493503519143	35.023489373027154	15.584098174244975	14.177477417536435
7	30.642816139893608	31.312408623928604	18.88559724219369	19.159177993984102
8	28.130244992557547	35.30599662715634	17.805826112657257	18.75793226762886
9	26.973322389701398	15.417716313645382	18.188249414510118	39.4207118821431
10	16.355670266809536	28.43937902132504	31.21195151592148	23.99299919594395
11	36.92169120019418	22.281348913268435	20.969788437819403	19.82717144871798
12	23.871839148670134	24.252102497922994	28.566891278582858	23.309167074824018
13	29.10128373709016	20.580949792262405	24.768131946387754	25.54963452425968
14	22.76059251656965	19.85859137986807	25.102304814657096	32.27851128890518
15	24.70466358667883	27.90735157606384	22.06910711562795	25.31887772162938
16	25.01211125706657	26.86743213600449	23.545955408507936	24.574501198421007
17	23.88096627143662	26.862526474506744	25.119780949053606	24.136726305003034
18	23.88027115357289	26.64453304579687	25.81833379305733	23.656862007572908
19	24.945351518956954	25.86476331473714	25.64991404022957	23.53997112607634
20	24.93085285105572	25.475571573525997	25.757910590972845	23.835664984445444
21	25.124862281175446	25.190465761302978	25.589938124750265	24.09473383277131
22	24.86815807791294	24.912389563375395	25.361758721425893	24.85769363728577
23	24.551213919832513	25.07966364572805	25.554519488882192	24.81460294555724
24	24.388960328143565	25.539087748580187	25.490974915125484	24.58097700815076
25	24.530296291135915	25.20109106303622	25.54600041799075	24.72261222783711
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	461.0
1	461.0
2	520.0
3	579.0
4	579.0
5	579.0
6	858.0
7	1137.0
8	1137.0
9	1137.0
10	1792.0
11	2447.0
12	2447.0
13	2447.0
14	4072.5
15	5698.0
16	5698.0
17	5698.0
18	10855.0
19	16012.0
20	16012.0
21	16012.0
22	26726.5
23	37441.0
24	37441.0
25	37441.0
26	56155.0
27	74869.0
28	74869.0
29	74869.0
30	99171.5
31	123474.0
32	123474.0
33	123474.0
34	153356.0
35	183238.0
36	183238.0
37	183238.0
38	222385.5
39	261533.0
40	261533.0
41	261533.0
42	294772.0
43	328011.0
44	328011.0
45	328011.0
46	360265.5
47	392520.0
48	392520.0
49	392520.0
50	407899.0
51	423278.0
52	423278.0
53	423278.0
54	406601.0
55	389924.0
56	389924.0
57	389924.0
58	347716.5
59	305509.0
60	305509.0
61	305509.0
62	260238.0
63	214967.0
64	214967.0
65	214967.0
66	170222.5
67	125478.0
68	125478.0
69	125478.0
70	94270.0
71	63062.0
72	63062.0
73	63062.0
74	44200.5
75	25339.0
76	25339.0
77	25339.0
78	18048.5
79	10758.0
80	10758.0
81	10758.0
82	7303.5
83	3849.0
84	3849.0
85	3849.0
86	2524.0
87	1199.0
88	1199.0
89	1199.0
90	724.5
91	250.0
92	250.0
93	250.0
94	150.0
95	50.0
96	50.0
97	50.0
98	26.0
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.09738940885999563
2	0.0
3	0.04078787463412106
4	0.05553168833383204
5	0.0706098288748063
6	0.005282364091959941
7	4.011922095159449E-4
8	0.004580277725307037
9	0.0
10	0.0
11	0.004814306514191339
12	0.0
13	0.0017384995745690945
14	0.05797227427505404
15	0.01995931242341826
16	0.0022065571523376968
17	0.0
18	0.0053492294602125985
19	0.00504833530307564
20	0.0
21	0.013038746809268208
22	1.0029805237898622E-4
23	0.0024071532570956694
24	0.006653104141139419
25	0.17963381181076432
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2991085.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.557837107764634
#Duplication Level	Percentage of deduplicated	Percentage of total
1	63.604017717295925	25.796413899774638
2	15.030656763393816	12.192218572648942
3	7.238707373728129	8.807589436033208
4	4.107700697443452	6.663978230974508
5	2.5201474993087283	5.110586588225189
6	1.6861535211762317	4.103204391032962
7	1.1738972953558755	3.332751470040235
8	0.8690170468268699	2.8196361463259887
9	0.6459746887669338	2.357940258247343
>10	2.9387260804035438	20.335327350949438
>50	0.1282229811947567	3.527179050648617
>100	0.05420138424389359	3.778790647772712
>500	0.0015794214961684394	0.4133758706451927
>1k	9.975293658174254E-4	0.7610080866811465
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	4525	0.15128289567163755	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	3.343268412632874E-5
6	0.0	0.0	0.0	3.343268412632874E-5	3.343268412632874E-5
7	0.0	0.0	0.0	3.343268412632874E-5	3.343268412632874E-5
8	0.0	0.0	0.0	3.343268412632874E-5	3.343268412632874E-5
9	0.0	0.0	0.0	3.343268412632874E-5	3.343268412632874E-5
10	0.0	0.0	0.0	3.343268412632874E-5	3.343268412632874E-5
11	0.0	0.0	0.0	3.343268412632874E-5	3.343268412632874E-5
12	0.0	0.0	0.0	6.686536825265748E-5	3.343268412632874E-4
13	0.0	0.0	0.0	6.686536825265748E-5	3.6775952538961614E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1545	0.0	12.436601	1
ACGATCG	65	8.03921E-4	11.690229	5
GTATCAA	3005	0.0	10.066105	1
CTTAGAC	265	0.0	10.039477	3
TATACCG	105	4.115692E-5	9.95061	5
CCCTTAA	500	0.0	9.892639	1
AGGACCG	260	0.0	9.86363	5
ACCGTCC	145	1.6220656E-7	9.826167	8
ACACCGT	185	6.5847416E-10	9.74964	6
GTCTTAA	255	0.0	9.698666	1
TTAGACA	295	0.0	9.662532	4
GTCTACG	100	2.721578E-4	9.512153	1
GTCTTAG	260	0.0	9.512153	1
GTACCGA	90	0.001121607	9.493071	6
GACCGTC	180	4.2400643E-9	9.493071	7
CTAAACT	215	1.05501385E-10	9.280524	4
CTATTAG	175	2.617162E-8	9.240376	1
CTTATAC	345	0.0	9.098581	1
CTTAGGC	295	0.0	9.018514	3
GGACCGT	180	4.2726242E-8	8.965677	6
>>END_MODULE