FastQCFastQC Report
Mon 15 Aug 2016
SRR2921199_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921199_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2419292
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGATTG802.8783355E-511.8707885
CTTAGAC1701.8189894E-1211.7308893
GGTATCA14750.011.4660291
GTATAGA2450.011.2464891
GTCTTAC1201.2737837E-711.0849021
ACGATTC1158.8650813E-710.7350998
CCCCTAG1252.2372478E-710.6415051
GTATCAA29900.010.2639941
GTCCTAG2250.010.1347681
GTATCGC750.002657199310.129326
ACCGTGC1606.215487E-910.0899618
GTGTAGA3500.09.7728121
CGACGAT1002.76208E-49.49800410
TAGGCTG2201.6370905E-119.496635
GTATTAG1451.7036637E-69.1737121
TAACCTG1451.7151851E-69.169165
TCGCGCA1151.1150169E-49.08392118
CCGTATG1054.5097098E-49.044419
GTACTAC1901.05574145E-89.0012741
TACGATT950.00183080858.9964357
Produced by FastQC (version 0.11.5)