##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921199_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2419292
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.506485368446636	31.0	28.0	31.0	16.0	34.0
2	27.317944671416267	31.0	27.0	31.0	10.0	34.0
3	27.075503907754833	31.0	27.0	31.0	10.0	34.0
4	31.03142696292965	35.0	32.0	37.0	16.0	37.0
5	30.702410043930207	35.0	31.0	37.0	10.0	37.0
6	31.104709559656296	35.0	32.0	37.0	15.0	37.0
7	31.058954024565864	35.0	32.0	37.0	11.0	37.0
8	30.668791944089428	35.0	31.0	37.0	10.0	37.0
9	32.35914143476687	37.0	32.0	39.0	10.0	39.0
10	31.706681128197836	37.0	31.0	39.0	10.0	39.0
11	32.17632596643977	37.0	32.0	39.0	10.0	39.0
12	31.87377381481855	37.0	32.0	39.0	8.0	39.0
13	31.89632669392533	37.0	32.0	39.0	8.0	39.0
14	33.02144057021641	38.0	32.0	40.0	8.0	41.0
15	32.664223252091936	38.0	32.0	40.0	2.0	41.0
16	32.58900289836861	38.0	32.0	40.0	2.0	41.0
17	32.91114383877597	38.0	32.0	40.0	2.0	41.0
18	32.95987090438029	38.0	32.0	40.0	2.0	41.0
19	32.666575593190075	38.0	32.0	40.0	2.0	41.0
20	32.53483043799591	38.0	32.0	40.0	2.0	41.0
21	32.493328626722196	38.0	32.0	40.0	2.0	41.0
22	31.7195861433841	38.0	30.0	40.0	2.0	41.0
23	31.82806788101643	38.0	31.0	40.0	2.0	41.0
24	31.470492606927976	38.0	30.0	40.0	2.0	41.0
25	30.963819993617967	38.0	30.0	40.0	2.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	171766.0
3	8.0
4	1504.0
5	8302.0
6	9745.0
7	8695.0
8	7485.0
9	7009.0
10	6576.0
11	6338.0
12	6234.0
13	5903.0
14	6154.0
15	6251.0
16	6665.0
17	7282.0
18	8413.0
19	10409.0
20	13102.0
21	16403.0
22	20000.0
23	23982.0
24	27681.0
25	31361.0
26	35548.0
27	39860.0
28	44586.0
29	52702.0
30	62278.0
31	74094.0
32	93321.0
33	118621.0
34	151927.0
35	197261.0
36	261048.0
37	348675.0
38	522103.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.03025268527986	17.053351653508862	12.291318907917963	25.625076753293314
2	16.02867617714384	19.30803317569845	38.79989330179257	25.86339734536514
3	19.442573789582955	22.65311361716852	28.9428960383044	28.96141655494413
4	13.902876307492356	15.276851675396719	34.57914485876416	36.241127158346764
5	14.521395663200451	35.786843263823	33.84687885608696	15.844882216889584
6	34.53666755122809	33.22247381243495	17.0885693996308	15.152289236706157
7	30.24625092850981	30.911016414937343	19.723925150432024	19.118807506120827
8	26.738027596144427	33.59085105266051	20.259493537462628	19.411627813732434
9	27.19292407651832	15.6727224174942	18.692854010093697	38.44149949589378
10	16.16633177814438	27.59943006258647	32.29380794498046	23.940430214288696
11	36.03802178886283	22.361570775772478	21.58088724672741	20.019520188637284
12	24.08534934374164	24.11362440385715	28.580794537456804	23.220231714944404
13	29.017653962168843	20.723015877280844	24.973409719331475	25.28592044121884
14	23.234810792486684	20.15750928622662	25.310750112137516	31.296929809149184
15	25.007357998888857	27.24026263922326	22.613445461520147	25.138933900367732
16	24.68264800628812	26.688329972475007	24.301341848702247	24.327680172534627
17	24.00075092024946	26.544741366691394	25.164594606440875	24.289913106618272
18	23.848943899697243	26.49364942406368	25.88271579162355	23.774690884615527
19	25.08412817578696	25.66082992135359	26.021526891251	23.23351501160845
20	25.525201876699878	25.244292200632763	25.473402049751808	23.757103872915554
21	25.806532966642248	25.062428992189346	25.1626461293198	23.96839191184861
22	24.649474713277982	24.37508138172213	26.533521002168115	24.441922902831774
23	25.052813480563398	24.708233942014726	25.566520180744064	24.672432396677817
24	24.732254510654215	24.996165763054492	25.560987104148623	24.710592622142666
25	24.290396889370065	24.770753832743424	26.335371123184746	24.60347815470176
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	851.0
1	851.0
2	930.5
3	1010.0
4	1010.0
5	1010.0
6	1175.0
7	1340.0
8	1340.0
9	1340.0
10	1801.5
11	2263.0
12	2263.0
13	2263.0
14	3252.5
15	4242.0
16	4242.0
17	4242.0
18	7673.0
19	11104.0
20	11104.0
21	11104.0
22	19016.0
23	26928.0
24	26928.0
25	26928.0
26	42994.0
27	59060.0
28	59060.0
29	59060.0
30	81381.0
31	103702.0
32	103702.0
33	103702.0
34	129109.0
35	154516.0
36	154516.0
37	154516.0
38	186174.5
39	217833.0
40	217833.0
41	217833.0
42	248299.0
43	278765.0
44	278765.0
45	278765.0
46	305369.0
47	331973.0
48	331973.0
49	331973.0
50	339839.0
51	347705.0
52	347705.0
53	347705.0
54	329185.5
55	310666.0
56	310666.0
57	310666.0
58	275710.5
59	240755.0
60	240755.0
61	240755.0
62	201734.5
63	162714.0
64	162714.0
65	162714.0
66	127112.5
67	91511.0
68	91511.0
69	91511.0
70	68169.0
71	44827.0
72	44827.0
73	44827.0
74	31171.5
75	17516.0
76	17516.0
77	17516.0
78	12105.5
79	6695.0
80	6695.0
81	6695.0
82	4542.0
83	2389.0
84	2389.0
85	2389.0
86	1540.5
87	692.0
88	692.0
89	692.0
90	448.5
91	205.0
92	205.0
93	205.0
94	115.0
95	25.0
96	25.0
97	25.0
98	15.0
99	5.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.034266223341374254
2	0.05195734950555782
3	0.01459104564475888
4	0.010457604952192626
5	0.009382910372125398
6	5.786816969592757E-4
7	0.003844099844086617
8	0.0016947106839521643
9	0.006572170701180345
10	0.0032654181471273415
11	0.010664276986820938
12	0.008225546978206847
13	0.007233521211990946
14	0.015996415480231407
15	0.006448167480403358
16	0.031538152484280525
17	0.03827566081316352
18	0.022113907705229464
19	0.015045724120941168
20	0.01508705852786683
21	0.022485917367560428
22	0.005662813748815769
23	0.016616431584116347
24	0.012276318856921776
25	0.4667067885976558
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2419292.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.90882856625127
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.27341121020893	31.80261159448911
2	12.772657791025694	11.727555137271843
3	6.111223787900763	8.416773756261984
4	3.485675617403735	6.400931374278006
5	2.133449739236545	4.8972089166662025
6	1.3944118513268804	3.8409488779988883
7	0.9713825908837083	3.121652578598481
8	0.7149411746157319	2.625768945631036
9	0.513597393881655	2.1220789237007693
>10	2.505621119557835	19.248553189633412
>50	0.09543155989025065	2.9773796769310703
>100	0.02642460854782194	2.0250982932699038
>500	0.0010256374065536916	0.3446681428781588
>1k	7.459181138568424E-4	0.44877059239111766
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.8934084847963786E-4
2	0.0	0.0	0.0	0.0	2.8934084847963786E-4
3	0.0	0.0	0.0	0.0	2.8934084847963786E-4
4	0.0	4.1334406925662546E-5	0.0	0.0	2.8934084847963786E-4
5	0.0	8.266881385132509E-5	0.0	0.0	2.8934084847963786E-4
6	0.0	8.266881385132509E-5	0.0	0.0	3.3067525540530037E-4
7	0.0	8.266881385132509E-5	0.0	0.0	3.3067525540530037E-4
8	0.0	1.2400322077698765E-4	0.0	0.0	3.3067525540530037E-4
9	0.0	1.2400322077698765E-4	0.0	0.0	3.3067525540530037E-4
10	0.0	1.2400322077698765E-4	0.0	0.0	4.133440692566255E-4
11	0.0	1.2400322077698765E-4	0.0	0.0	4.133440692566255E-4
12	0.0	1.2400322077698765E-4	0.0	0.0	9.506913592902387E-4
13	0.0	1.2400322077698765E-4	0.0	0.0	0.0010746945800672262
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGATTG	80	2.8783355E-5	11.870788	5
CTTAGAC	170	1.8189894E-12	11.730889	3
GGTATCA	1475	0.0	11.466029	1
GTATAGA	245	0.0	11.246489	1
GTCTTAC	120	1.2737837E-7	11.084902	1
ACGATTC	115	8.8650813E-7	10.735099	8
CCCCTAG	125	2.2372478E-7	10.641505	1
GTATCAA	2990	0.0	10.263994	1
GTCCTAG	225	0.0	10.134768	1
GTATCGC	75	0.0026571993	10.12932	6
ACCGTGC	160	6.215487E-9	10.089961	8
GTGTAGA	350	0.0	9.772812	1
CGACGAT	100	2.76208E-4	9.498004	10
TAGGCTG	220	1.6370905E-11	9.49663	5
GTATTAG	145	1.7036637E-6	9.173712	1
TAACCTG	145	1.7151851E-6	9.16916	5
TCGCGCA	115	1.1150169E-4	9.083921	18
CCGTATG	105	4.5097098E-4	9.04441	9
GTACTAC	190	1.05574145E-8	9.001274	1
TACGATT	95	0.0018308085	8.996435	7
>>END_MODULE