FastQCFastQC Report
Mon 15 Aug 2016
SRR2921199_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921199_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2419292
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT40980.16938839958136515No Hit
GGTATCAACGCAGAGTACTTTTTTT24410.10089728730554227No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCAA707.2625353E-613.57215310
ACGATTC802.0055613E-613.0596848
TACACCG1051.9968866E-812.6660345
GGTATCA15200.011.7060411
CGATTCG658.02266E-411.6929329
TATCGGA1001.9205072E-611.4050912
CTAGTAC700.001486375110.8619913
ATTACGA700.001492544710.85665
TCGACGC700.001500546610.84964318
GTATCAA32250.010.6804841
CGTACAC1355.6543286E-810.5602683
GTCCTAG2450.010.4859971
GTCTTAA1752.2919266E-1010.3306491
TAGGACC1752.3464963E-1010.3188924
GTATTAG1409.472569E-810.1947191
ACACCGT2450.010.0738366
TAGGGTG2002.5465852E-119.9745015
TAAGACT2105.456968E-129.9568254
ACCGTCC1151.0295291E-59.9109078
AGAACCG1356.450573E-79.8513585
Produced by FastQC (version 0.11.5)