##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921199_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2419292
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.900157153415133	31.0	30.0	33.0	26.0	34.0
2	30.138950569009445	31.0	30.0	34.0	26.0	34.0
3	30.26235650760636	31.0	30.0	34.0	26.0	34.0
4	33.96894504673268	35.0	35.0	37.0	30.0	37.0
5	33.57320943482639	35.0	35.0	37.0	28.0	37.0
6	33.57241085408458	37.0	35.0	37.0	28.0	37.0
7	33.4433751692644	37.0	35.0	37.0	28.0	37.0
8	33.39611588845001	37.0	35.0	37.0	28.0	37.0
9	34.8154286460667	39.0	35.0	39.0	27.0	39.0
10	34.69355993406335	39.0	35.0	39.0	27.0	39.0
11	34.741044074051416	39.0	35.0	39.0	27.0	39.0
12	34.59942826248341	38.0	35.0	39.0	27.0	39.0
13	34.58661872977714	39.0	35.0	39.0	27.0	39.0
14	35.581469289362346	39.0	36.0	41.0	27.0	41.0
15	35.580532238357335	39.0	36.0	41.0	27.0	41.0
16	35.567856629129516	39.0	36.0	41.0	27.0	41.0
17	35.542307418864695	39.0	36.0	41.0	27.0	41.0
18	35.49232089388135	39.0	36.0	41.0	27.0	41.0
19	35.53304975174555	39.0	36.0	41.0	27.0	41.0
20	35.46496123659318	39.0	36.0	41.0	27.0	41.0
21	35.39690744234264	39.0	35.0	41.0	26.0	41.0
22	35.31734077573108	39.0	35.0	41.0	26.0	41.0
23	35.19786491254466	39.0	35.0	41.0	25.0	41.0
24	35.126223705117035	39.0	35.0	41.0	25.0	41.0
25	34.994506657319576	39.0	35.0	41.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	62227.0
3	7.0
4	1048.0
5	8479.0
6	9619.0
7	7319.0
8	5680.0
9	4961.0
10	4621.0
11	4277.0
12	3997.0
13	3842.0
14	3659.0
15	3760.0
16	3861.0
17	4016.0
18	4561.0
19	5410.0
20	6757.0
21	8710.0
22	11088.0
23	13669.0
24	16440.0
25	19254.0
26	22427.0
27	25233.0
28	29459.0
29	35565.0
30	45927.0
31	59295.0
32	75866.0
33	98986.0
34	130346.0
35	175262.0
36	238593.0
37	363777.0
38	901294.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.040550683596095	18.29381929163539	9.556773522433588	31.108856502334923
2	15.174563467328458	20.709984573999336	35.21158256217108	28.903869396501126
3	18.824777762753133	23.138725582022364	27.2896215402136	30.7468751150109
4	13.95481658272026	15.956379023916764	32.62739499530813	37.46140939805485
5	15.347632248836208	36.75402043245864	32.084618738330015	15.813728580375145
6	34.920111987480155	33.96632521756168	16.199452794085975	14.914110000872189
7	30.813862408449417	30.954027440270725	18.968491181920655	19.263618969359207
8	27.99584330035999	34.44496940551672	18.430969892449117	19.12821740167417
9	27.361269330035398	15.627216557571389	18.442048334802084	38.56946577759113
10	17.144395963777832	28.114423558627895	30.53265170140686	24.208528776187414
11	36.44429269817046	22.234599831434878	21.243008660146998	20.078098810247667
12	24.410736694867754	24.230353343044165	28.04093098311407	23.317978978974015
13	29.267154469797234	20.480621936591103	24.84770274817074	25.40452084544092
14	23.364205355957466	20.096235013944316	25.095757313062823	31.44380231703539
15	25.175319858999917	27.40505109723234	22.19002431862777	25.22960472513997
16	25.327544851024292	26.660168884468856	23.470262106149455	24.5420241583574
17	24.110152887704338	26.552809664976362	25.103336017314152	24.23370143000514
18	24.028503272320833	26.343247089674364	25.894261440928958	23.73398819707585
19	25.34068775772727	25.553400594570817	25.596678935063476	23.509232712638433
20	25.49572354225947	25.13045138825739	25.440914118676044	23.932910950807095
21	25.549775013590132	24.97880353116015	25.30318908159659	24.168232373653133
22	25.50672903755687	24.532104653801465	25.014105372193757	24.947060936447908
23	25.10052577626401	24.69834000198406	25.361760523792203	24.839373697959726
24	24.850013764755776	25.08033574430127	25.284574958395716	24.78507553254724
25	24.915856286512657	24.840781211437584	25.440222351419262	24.803140150630497
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	545.0
1	545.0
2	588.0
3	631.0
4	631.0
5	631.0
6	833.0
7	1035.0
8	1035.0
9	1035.0
10	1403.5
11	1772.0
12	1772.0
13	1772.0
14	2404.0
15	3036.0
16	3036.0
17	3036.0
18	6414.5
19	9793.0
20	9793.0
21	9793.0
22	17401.0
23	25009.0
24	25009.0
25	25009.0
26	40467.0
27	55925.0
28	55925.0
29	55925.0
30	75985.0
31	96045.0
32	96045.0
33	96045.0
34	119476.5
35	142908.0
36	142908.0
37	142908.0
38	172926.0
39	202944.0
40	202944.0
41	202944.0
42	234412.5
43	265881.0
44	265881.0
45	265881.0
46	295186.0
47	324491.0
48	324491.0
49	324491.0
50	338688.5
51	352886.0
52	352886.0
53	352886.0
54	338358.5
55	323831.0
56	323831.0
57	323831.0
58	288996.0
59	254161.0
60	254161.0
61	254161.0
62	215961.5
63	177762.0
64	177762.0
65	177762.0
66	139118.5
67	100475.0
68	100475.0
69	100475.0
70	75041.0
71	49607.0
72	49607.0
73	49607.0
74	34435.0
75	19263.0
76	19263.0
77	19263.0
78	13296.5
79	7330.0
80	7330.0
81	7330.0
82	5077.0
83	2824.0
84	2824.0
85	2824.0
86	1817.0
87	810.0
88	810.0
89	810.0
90	541.0
91	272.0
92	272.0
93	272.0
94	161.0
95	50.0
96	50.0
97	50.0
98	28.0
99	6.0
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.10391469901111564
2	0.0
3	0.04311178642346604
4	0.05294937527177372
5	0.07299656263072006
6	0.003844099844086617
7	4.1334406925662546E-5
8	0.002686736450168066
9	0.0
10	0.0
11	0.002025385939357465
12	0.0
13	4.133440692566255E-4
14	0.06038956851839298
15	0.023560611947627652
16	2.0667203462831275E-4
17	0.0
18	0.0025213988224654154
19	0.0028107396709450535
20	0.0
21	0.010622942579895276
22	4.1334406925662546E-5
23	4.960128831079506E-4
24	0.0028934084847963785
25	0.18087936470669932
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2419292.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.81665568146402
#Duplication Level	Percentage of deduplicated	Percentage of total
1	58.611653758340054	19.820501140669723
2	15.008382547628349	10.150666098976831
3	7.839853058313037	7.95352834398734
4	4.753743421203209	6.430228178914149
5	3.1205005513124875	5.276244634877652
6	2.1785254890842105	4.420226781459225
7	1.599203488359131	3.785579961130582
8	1.1749980558411282	3.178760374461525
9	0.886896546357167	2.699268761991592
>10	4.551371635938579	26.451641238365557
>50	0.2038745526134743	4.669065993548603
>100	0.06704313657483103	3.662972192251014
>500	0.002471099021435381	0.5564964875670785
>1k	0.001482659412861117	0.9448198117992037
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	4098	0.16938839958136515	No Hit
GGTATCAACGCAGAGTACTTTTTTT	2441	0.10089728730554227	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.0667203462831275E-4
2	0.0	4.1334406925662546E-5	0.0	0.0	2.0667203462831275E-4
3	0.0	4.1334406925662546E-5	0.0	0.0	2.0667203462831275E-4
4	0.0	4.1334406925662546E-5	0.0	0.0	2.0667203462831275E-4
5	0.0	4.1334406925662546E-5	0.0	0.0	2.0667203462831275E-4
6	0.0	4.1334406925662546E-5	0.0	0.0	2.480064415539753E-4
7	0.0	4.1334406925662546E-5	0.0	0.0	2.480064415539753E-4
8	0.0	4.1334406925662546E-5	0.0	0.0	2.480064415539753E-4
9	0.0	4.1334406925662546E-5	0.0	0.0	2.480064415539753E-4
10	0.0	4.1334406925662546E-5	0.0	0.0	2.480064415539753E-4
11	0.0	1.2400322077698765E-4	0.0	0.0	2.480064415539753E-4
12	0.0	1.2400322077698765E-4	0.0	0.0	9.920257662159012E-4
13	0.0	1.2400322077698765E-4	0.0	0.0	0.0012400322077698765
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGATCAA	70	7.2625353E-6	13.572153	10
ACGATTC	80	2.0055613E-6	13.059684	8
TACACCG	105	1.9968866E-8	12.666034	5
GGTATCA	1520	0.0	11.706041	1
CGATTCG	65	8.02266E-4	11.692932	9
TATCGGA	100	1.9205072E-6	11.405091	2
CTAGTAC	70	0.0014863751	10.861991	3
ATTACGA	70	0.0014925447	10.8566	5
TCGACGC	70	0.0015005466	10.849643	18
GTATCAA	3225	0.0	10.680484	1
CGTACAC	135	5.6543286E-8	10.560268	3
GTCCTAG	245	0.0	10.485997	1
GTCTTAA	175	2.2919266E-10	10.330649	1
TAGGACC	175	2.3464963E-10	10.318892	4
GTATTAG	140	9.472569E-8	10.194719	1
ACACCGT	245	0.0	10.073836	6
TAGGGTG	200	2.5465852E-11	9.974501	5
TAAGACT	210	5.456968E-12	9.956825	4
ACCGTCC	115	1.0295291E-5	9.910907	8
AGAACCG	135	6.450573E-7	9.851358	5
>>END_MODULE