FastQCFastQC Report
Mon 15 Aug 2016
SRR2921198_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921198_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2124675
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9350.012.5026731
TCGCTAA550.003072246712.08924511
ATAACCC1052.734687E-711.7575193
GTATAAA1657.2759576E-1211.5200771
ACCGTAT855.3450254E-511.1723038
TAGACAG3100.011.0273665
ACCGTCC1053.48309E-610.8530948
GTATCAA21500.010.7860071
TTATGGC1659.367795E-1010.3585524
GTATTAG1606.1318133E-910.0980671
GGATATA1701.54796E-910.0631271
TATCGTG951.6467505E-49.99862511
GTATAAT1151.021501E-59.9172841
GTATTAC1752.5556801E-99.7756091
GTCTTAG1901.0440999E-99.5040641
CCTAATA1502.6468297E-79.5004832
ATATAAC800.0045305169.4964583
TAGGTTG900.00111839579.4957875
GTGTAGC2054.2018655E-109.2722581
TAGACTG1856.7484507E-99.2391445
Produced by FastQC (version 0.11.5)