##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921198_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2124675
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.21032251991481	31.0	28.0	31.0	10.0	34.0
2	26.975614152752772	31.0	27.0	31.0	10.0	34.0
3	26.80257168743455	31.0	26.0	31.0	10.0	34.0
4	30.755473190017298	35.0	31.0	37.0	10.0	37.0
5	30.522888912421898	35.0	31.0	37.0	10.0	37.0
6	30.792227987809902	35.0	32.0	37.0	10.0	37.0
7	30.77461541176886	35.0	32.0	37.0	10.0	37.0
8	30.35840210855778	35.0	30.0	37.0	8.0	37.0
9	32.03137091555179	37.0	32.0	39.0	8.0	39.0
10	31.373234494687424	37.0	30.0	39.0	8.0	39.0
11	31.82074434913575	37.0	32.0	39.0	2.0	39.0
12	31.530419946580064	37.0	31.0	39.0	2.0	39.0
13	31.536672667584455	37.0	31.0	39.0	2.0	39.0
14	32.64643721981009	38.0	32.0	40.0	2.0	41.0
15	32.293243437231574	38.0	31.0	40.0	2.0	41.0
16	32.211255839128334	38.0	31.0	40.0	2.0	41.0
17	32.5205887959335	38.0	32.0	40.0	2.0	41.0
18	32.56836975066775	38.0	32.0	40.0	2.0	41.0
19	32.27523456528645	38.0	31.0	40.0	2.0	41.0
20	32.139244355019	38.0	31.0	40.0	2.0	41.0
21	32.092015484721195	38.0	31.0	40.0	2.0	41.0
22	31.329665478249616	38.0	30.0	40.0	2.0	41.0
23	31.436932707355243	38.0	30.0	40.0	2.0	41.0
24	31.070310047419017	38.0	30.0	40.0	2.0	41.0
25	30.571605539670774	38.0	30.0	40.0	2.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	167044.0
3	10.0
4	1403.0
5	7955.0
6	9298.0
7	8099.0
8	6781.0
9	6390.0
10	6129.0
11	5752.0
12	5707.0
13	5582.0
14	5528.0
15	5791.0
16	6084.0
17	6673.0
18	7754.0
19	9338.0
20	11786.0
21	14747.0
22	17776.0
23	21537.0
24	24872.0
25	27751.0
26	31213.0
27	35314.0
28	39580.0
29	46232.0
30	54491.0
31	65403.0
32	81729.0
33	102857.0
34	131435.0
35	169797.0
36	226098.0
37	302013.0
38	448726.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.42774597500581	16.85374795065951	11.693835828639822	26.024670245694853
2	16.651801859497905	19.197498191736234	38.097009583797956	26.053690364967903
3	19.7524680699627	22.717070589719498	28.524543244652907	29.005918095664896
4	14.100145967947302	15.187162352037408	33.753899264699996	36.95879241531529
5	14.713757040632558	35.5505461638614	33.86719599482973	15.868500800676316
6	34.82162061923407	32.992760713846984	16.8763240372446	15.30929462967434
7	30.530381976313457	30.144847315848484	20.038153387348665	19.2866173204894
8	27.239194098161278	32.99978443264838	20.36993805027332	19.391083418917024
9	27.27388688531226	15.558141220615909	18.369016452549126	38.7989554415227
10	16.29477618087212	27.13505397499288	32.224965228667116	24.34520461546789
11	36.468909174524825	21.964548725144592	21.561194801257933	20.00534729907265
12	24.389460925640453	23.687875828145778	28.432200896692123	23.490462349521646
13	29.365013243109793	20.51912425553106	24.74082149172912	25.375041009630028
14	23.56487248070664	19.75012074531635	24.918161507805372	31.766845266171632
15	25.214353897626008	26.882021572832315	22.338274361692314	25.565350167849367
16	25.159315889214547	26.042915828007107	24.073448921218972	24.724319361559377
17	24.310255933364566	26.218820554714554	24.746832909955998	24.72409060196488
18	24.34659469404338	25.831524366791093	25.4848901273503	24.336990811815223
19	25.45724323273425	25.20068897762894	25.556899261735712	23.7851685279011
20	25.711252803845003	24.776504426132657	25.20770224754803	24.3045405224743
21	26.078169399987573	24.506815883579172	24.87439717233574	24.540617544097515
22	24.86099324229232	24.013043783186642	26.2322567222882	24.89370625223284
23	25.346798550855627	24.283430495300074	25.207128223668352	25.162642730175943
24	25.05285098775213	24.74697192436907	25.256676217738068	24.943500870140735
25	24.681449744157234	24.436956747912113	25.86215702408989	25.019436483840764
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	582.0
1	582.0
2	605.5
3	629.0
4	629.0
5	629.0
6	801.5
7	974.0
8	974.0
9	974.0
10	1335.5
11	1697.0
12	1697.0
13	1697.0
14	2424.0
15	3151.0
16	3151.0
17	3151.0
18	5771.5
19	8392.0
20	8392.0
21	8392.0
22	14435.0
23	20478.0
24	20478.0
25	20478.0
26	32959.0
27	45440.0
28	45440.0
29	45440.0
30	63831.5
31	82223.0
32	82223.0
33	82223.0
34	105247.5
35	128272.0
36	128272.0
37	128272.0
38	156627.5
39	184983.0
40	184983.0
41	184983.0
42	211736.0
43	238489.0
44	238489.0
45	238489.0
46	262153.5
47	285818.0
48	285818.0
49	285818.0
50	296021.0
51	306224.0
52	306224.0
53	306224.0
54	293961.5
55	281699.0
56	281699.0
57	281699.0
58	250636.0
59	219573.0
60	219573.0
61	219573.0
62	186717.0
63	153861.0
64	153861.0
65	153861.0
66	121842.0
67	89823.0
68	89823.0
69	89823.0
70	66889.5
71	43956.0
72	43956.0
73	43956.0
74	30913.0
75	17870.0
76	17870.0
77	17870.0
78	12389.5
79	6909.0
80	6909.0
81	6909.0
82	4738.0
83	2567.0
84	2567.0
85	2567.0
86	1680.0
87	793.0
88	793.0
89	793.0
90	516.5
91	240.0
92	240.0
93	240.0
94	133.5
95	27.0
96	27.0
97	27.0
98	16.0
99	5.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.038641203948839235
2	0.051349029851624366
3	0.016143645498723335
4	0.01110758115947145
5	0.01040159083153894
6	8.471883935190087E-4
7	0.004377140033181545
8	0.0023062350712461906
9	0.006636309082565569
10	0.003765281748973372
11	0.011201713203195783
12	0.009413204372433431
13	0.008189487804017084
14	0.01736736206713968
15	0.007106969301187241
16	0.03529951639662537
17	0.041418099238707096
18	0.02513325567439726
19	0.017320296045277513
20	0.016943767870380176
21	0.024427265346464753
22	0.006495111016979067
23	0.018214550460658688
24	0.01440220268982315
25	0.4751314906985774
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2124675.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.41581591819231
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.29771222286486	38.41959391098319
2	12.578199879482465	13.185932189303632
3	5.321079187417597	8.367261215214153
4	2.79121469900415	5.852151834046164
5	1.630786857720269	4.273951186803645
6	1.0234780226738371	3.2187861379672382
7	0.705208970245591	2.58748724977756
8	0.49465501628367137	2.074219702122827
9	0.3579042967345135	1.6883861160569704
>10	1.703404166311497	15.099343272299059
>50	0.06843830934965443	2.426932356536974
>100	0.026620829604184874	2.2463639584437924
>500	0.0011121791208886995	0.42303301500417323
>1k	1.853631868147784E-4	0.13655785544059593
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.41320437243343E-5
2	0.0	0.0	0.0	0.0	9.41320437243343E-5
3	0.0	0.0	0.0	0.0	9.41320437243343E-5
4	0.0	0.0	0.0	0.0	9.41320437243343E-5
5	0.0	0.0	0.0	0.0	9.41320437243343E-5
6	0.0	0.0	0.0	0.0	1.882640874486686E-4
7	0.0	0.0	0.0	0.0	1.882640874486686E-4
8	0.0	0.0	0.0	0.0	1.882640874486686E-4
9	0.0	0.0	0.0	0.0	1.882640874486686E-4
10	0.0	0.0	0.0	0.0	1.882640874486686E-4
11	0.0	0.0	0.0	0.0	1.882640874486686E-4
12	0.0	0.0	0.0	0.0	6.11858284208173E-4
13	0.0	0.0	0.0	0.0	6.589243060703402E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	935	0.0	12.502673	1
TCGCTAA	55	0.0030722467	12.089245	11
ATAACCC	105	2.734687E-7	11.757519	3
GTATAAA	165	7.2759576E-12	11.520077	1
ACCGTAT	85	5.3450254E-5	11.172303	8
TAGACAG	310	0.0	11.027366	5
ACCGTCC	105	3.48309E-6	10.853094	8
GTATCAA	2150	0.0	10.786007	1
TTATGGC	165	9.367795E-10	10.358552	4
GTATTAG	160	6.1318133E-9	10.098067	1
GGATATA	170	1.54796E-9	10.063127	1
TATCGTG	95	1.6467505E-4	9.998625	11
GTATAAT	115	1.021501E-5	9.917284	1
GTATTAC	175	2.5556801E-9	9.775609	1
GTCTTAG	190	1.0440999E-9	9.504064	1
CCTAATA	150	2.6468297E-7	9.500483	2
ATATAAC	80	0.004530516	9.496458	3
TAGGTTG	90	0.0011183957	9.495787	5
GTGTAGC	205	4.2018655E-10	9.272258	1
TAGACTG	185	6.7484507E-9	9.239144	5
>>END_MODULE