FastQCFastQC Report
Mon 15 Aug 2016
SRR2921198_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921198_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2124675
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT26860.126419334721781No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGTAC400.00526515714.2560263
GGTATCA11500.012.9063941
TATAGCG751.4809968E-512.665165
CGAATCG701.09825334E-412.20593416
AATCGTA701.09825334E-412.20593418
GCGAATC1302.6338967E-911.687613515
ACCGACC907.481787E-611.6086368
GGATCGT700.001501212210.8489536
CTTATAC2200.010.3792691
ATCGCTC1106.06283E-610.3614278
GTATCAA25900.010.359231
ACTATAC750.002615345410.1486181
CGTACAC856.5694767E-410.0630773
GAACCGC2151.0913936E-119.7135976
TACACTG4900.09.6927245
CTCTTAT1606.4841515E-89.5143291
GCTTTAA1606.4841515E-89.5143291
ATAGCAC4000.09.5040183
GTTACAC1502.6325506E-79.5040183
TCGGAAC1606.593473E-89.5040173
Produced by FastQC (version 0.11.5)