##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921198_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2124675
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.829474154870745	31.0	30.0	33.0	26.0	34.0
2	30.079702072081613	31.0	30.0	34.0	26.0	34.0
3	30.219486274371373	31.0	30.0	34.0	26.0	34.0
4	33.91877816607246	35.0	35.0	37.0	30.0	37.0
5	33.51725087366303	35.0	35.0	37.0	28.0	37.0
6	33.50523491828162	37.0	35.0	37.0	28.0	37.0
7	33.36990645628155	37.0	35.0	37.0	28.0	37.0
8	33.32534011084048	37.0	35.0	37.0	27.0	37.0
9	34.74375563321449	39.0	35.0	39.0	27.0	39.0
10	34.615402355654396	38.0	35.0	39.0	27.0	39.0
11	34.654098626848814	39.0	35.0	39.0	27.0	39.0
12	34.5208264793439	38.0	35.0	39.0	27.0	39.0
13	34.49959593820231	38.0	35.0	39.0	27.0	39.0
14	35.502793133067414	39.0	36.0	41.0	27.0	41.0
15	35.49466718439291	39.0	36.0	41.0	27.0	41.0
16	35.4796182945627	39.0	36.0	41.0	27.0	41.0
17	35.45021568004518	39.0	36.0	41.0	27.0	41.0
18	35.39588407638816	39.0	36.0	41.0	26.0	41.0
19	35.43054678950898	39.0	36.0	41.0	26.0	41.0
20	35.36522009248473	39.0	35.0	41.0	26.0	41.0
21	35.296613364396904	39.0	35.0	41.0	26.0	41.0
22	35.215261628249024	39.0	35.0	41.0	25.0	41.0
23	35.093936719733605	39.0	35.0	41.0	25.0	41.0
24	35.021256898113826	39.0	35.0	41.0	25.0	41.0
25	34.88677515384706	39.0	35.0	41.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	57823.0
3	9.0
4	873.0
5	7768.0
6	8748.0
7	6638.0
8	5307.0
9	4509.0
10	4031.0
11	3815.0
12	3520.0
13	3431.0
14	3418.0
15	3410.0
16	3430.0
17	3720.0
18	4041.0
19	4676.0
20	5957.0
21	7732.0
22	10045.0
23	12259.0
24	14912.0
25	17023.0
26	19440.0
27	22476.0
28	25666.0
29	31671.0
30	40676.0
31	51845.0
32	66299.0
33	86629.0
34	114253.0
35	151406.0
36	207454.0
37	318032.0
38	791733.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.2202159397374	17.895313139003903	9.059178946535221	31.82529197472348
2	15.424382552625696	20.516926118112185	34.39594290891549	29.66274842034664
3	18.95387075448777	23.12475162117214	26.850669469227395	31.070708155112698
4	14.103087559578556	15.849155410257895	32.103499152561135	37.94425787760242
5	15.547959980519657	36.415475756488185	31.97550262202767	16.061061640964482
6	35.32276861612497	33.66204515202235	16.11423751597981	14.90094871587287
7	31.14508659937148	30.3852035305264	19.079866087376434	19.389843782725684
8	28.371698527051436	34.053663261761606	18.26142742431217	19.313210786874784
9	27.569440032004895	15.464059209055502	17.949474625530964	39.01702613340864
10	17.213079647475496	27.816819042912442	30.376269311777094	24.593831997834965
11	36.92851941856103	21.966204318854782	21.067019487515477	20.038256775068707
12	24.64720486662666	23.844776259898573	27.817054373021755	23.69096450045301
13	29.555254015836013	20.24798425717918	24.524639576099794	25.67212215088502
14	23.700500819943127	19.590188563371104	24.650429186772776	32.058881429912994
15	25.34347213453873	27.054018210861265	21.818430905642202	25.7840787489578
16	25.778594430549422	26.029457862003465	23.248450812411576	24.943496895035537
17	24.435360702225044	26.250744231470698	24.633414522221045	24.680480544083213
18	24.554149271153495	25.78229211283118	25.357127728173435	24.30643088784189
19	25.71506032262437	25.0419377795852	25.03897248203877	24.20402941575166
20	25.74247826138115	24.78882652640992	24.995822890559733	24.472872321649195
21	26.011299211634743	24.35864123261388	24.885520402034274	24.744539153717106
22	25.70336639721369	24.265499429324485	24.673326508760162	25.35780766470166
23	25.42457681165559	24.25788778831287	24.87163737942103	25.445898020610503
24	25.161762830062308	24.85440521594814	24.872197126841733	25.11163482714782
25	25.32621698634116	24.502593656681054	24.84733002601437	25.323859330963415
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	332.0
1	332.0
2	347.5
3	363.0
4	363.0
5	363.0
6	542.0
7	721.0
8	721.0
9	721.0
10	997.5
11	1274.0
12	1274.0
13	1274.0
14	1703.0
15	2132.0
16	2132.0
17	2132.0
18	4479.5
19	6827.0
20	6827.0
21	6827.0
22	12356.0
23	17885.0
24	17885.0
25	17885.0
26	29467.5
27	41050.0
28	41050.0
29	41050.0
30	57680.5
31	74311.0
32	74311.0
33	74311.0
34	95416.0
35	116521.0
36	116521.0
37	116521.0
38	145196.0
39	173871.0
40	173871.0
41	173871.0
42	200622.5
43	227374.0
44	227374.0
45	227374.0
46	253761.0
47	280148.0
48	280148.0
49	280148.0
50	294795.0
51	309442.0
52	309442.0
53	309442.0
54	301614.5
55	293787.0
56	293787.0
57	293787.0
58	263070.5
59	232354.0
60	232354.0
61	232354.0
62	199867.0
63	167380.0
64	167380.0
65	167380.0
66	133055.5
67	98731.0
68	98731.0
69	98731.0
70	73770.5
71	48810.0
72	48810.0
73	48810.0
74	34187.5
75	19565.0
76	19565.0
77	19565.0
78	13572.5
79	7580.0
80	7580.0
81	7580.0
82	5239.0
83	2898.0
84	2898.0
85	2898.0
86	1923.5
87	949.0
88	949.0
89	949.0
90	620.5
91	292.0
92	292.0
93	292.0
94	179.0
95	66.0
96	66.0
97	66.0
98	39.0
99	12.0
100	12.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.10500429477449492
2	0.0
3	0.04250061774153694
4	0.05732641462811959
5	0.07102262699001023
6	0.005600856601597892
7	2.8239613117300293E-4
8	0.004377140033181545
9	0.0
10	0.0
11	0.003953545836422041
12	0.0
13	0.0011295845246920117
14	0.06386859166696084
15	0.026262840199089272
16	6.11858284208173E-4
17	0.0
18	0.004000611858284208
19	0.004659536164354548
20	0.0
21	0.013131420099544636
22	0.0
23	0.0014119806558650146
24	0.0053655264922870555
25	0.18468706978714391
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2124675.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.940861106056104
#Duplication Level	Percentage of deduplicated	Percentage of total
1	63.10149415683897	25.20328013702916
2	15.497312401593302	12.379520042983977
3	7.378182693073995	8.840729104775264
4	4.090408651820402	6.534977753174756
5	2.4562168749885607	4.9051708525134625
6	1.6115167941310273	3.8619221066678504
7	1.139871303676142	3.1869208993235523
8	0.8272414265562205	2.643258793540617
9	0.6316997979178505	2.270757050042451
>10	3.0544272926664933	21.028297734858455
>50	0.15033534056977368	4.0711111634605714
>100	0.058804688131879695	4.025995069554366
>500	0.0014220445916311609	0.41462310430299676
>1k	0.00106653344372337	0.633436187772489
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2686	0.126419334721781	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.41320437243343E-5
2	0.0	0.0	0.0	0.0	9.41320437243343E-5
3	0.0	0.0	0.0	0.0	9.41320437243343E-5
4	0.0	0.0	0.0	0.0	9.41320437243343E-5
5	0.0	0.0	0.0	0.0	9.41320437243343E-5
6	0.0	0.0	0.0	0.0	9.41320437243343E-5
7	0.0	0.0	0.0	0.0	9.41320437243343E-5
8	0.0	0.0	0.0	0.0	9.41320437243343E-5
9	0.0	0.0	0.0	0.0	9.41320437243343E-5
10	0.0	0.0	0.0	0.0	9.41320437243343E-5
11	0.0	0.0	0.0	0.0	9.41320437243343E-5
12	0.0	0.0	0.0	0.0	4.2359419675950437E-4
13	0.0	0.0	0.0	0.0	6.589243060703402E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAGTAC	40	0.005265157	14.256026	3
GGTATCA	1150	0.0	12.906394	1
TATAGCG	75	1.4809968E-5	12.66516	5
CGAATCG	70	1.09825334E-4	12.205934	16
AATCGTA	70	1.09825334E-4	12.205934	18
GCGAATC	130	2.6338967E-9	11.6876135	15
ACCGACC	90	7.481787E-6	11.608636	8
GGATCGT	70	0.0015012122	10.848953	6
CTTATAC	220	0.0	10.379269	1
ATCGCTC	110	6.06283E-6	10.361427	8
GTATCAA	2590	0.0	10.35923	1
ACTATAC	75	0.0026153454	10.148618	1
CGTACAC	85	6.5694767E-4	10.063077	3
GAACCGC	215	1.0913936E-11	9.713597	6
TACACTG	490	0.0	9.692724	5
CTCTTAT	160	6.4841515E-8	9.514329	1
GCTTTAA	160	6.4841515E-8	9.514329	1
ATAGCAC	400	0.0	9.504018	3
GTTACAC	150	2.6325506E-7	9.504018	3
TCGGAAC	160	6.593473E-8	9.504017	3
>>END_MODULE