FastQCFastQC Report
Mon 15 Aug 2016
SRR2921197_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921197_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1772515
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT23270.1312823868909431No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12300.013.9813581
CCCTACG1155.315087E-912.3927961
TACGAGA1053.4873538E-610.8517094
CTAGACA1502.1500455E-910.7612784
TGCACCG803.7877334E-410.6824525
GTAGTAC803.7887212E-410.6821513
TACACCG856.622614E-410.0540725
GTATCAA25400.010.0248281
TCAACTC2750.010.0134515
GTACGTG951.6421212E-410.0012041
AGACCGT1451.6279955E-79.8229456
GATATAA1651.0197255E-89.7890571
TAGACAG2054.1836756E-119.7271115
CCGATCG800.00452411839.49792618
TCTCGCA1002.761376E-49.49792518
TTACACT2054.274625E-109.2636544
CACCGTG1451.7155144E-69.1685997
ACCGTGC1151.114192E-49.0842038
TAATCTG1151.1162488E-49.08266455
CTCGCAA950.00175949019.03552519
Produced by FastQC (version 0.11.5)