##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921197_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1772515
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.45222974135621	31.0	26.0	31.0	2.0	34.0
2	26.072488526190188	31.0	26.0	31.0	2.0	34.0
3	25.851888982603814	31.0	25.0	31.0	2.0	34.0
4	29.65400574889352	35.0	30.0	37.0	2.0	37.0
5	29.27715816227225	35.0	28.0	37.0	2.0	37.0
6	29.755371322668637	35.0	30.0	37.0	2.0	37.0
7	29.574121516602116	35.0	30.0	37.0	2.0	37.0
8	29.28140918412538	35.0	29.0	37.0	2.0	37.0
9	30.87811668730589	37.0	30.0	39.0	2.0	39.0
10	30.212874362135157	37.0	28.0	39.0	2.0	39.0
11	30.674196269142996	37.0	30.0	39.0	2.0	39.0
12	30.37141575670728	37.0	30.0	39.0	2.0	39.0
13	30.361732340770036	37.0	30.0	39.0	2.0	39.0
14	31.392519668380803	38.0	30.0	40.0	2.0	41.0
15	31.06088806018567	38.0	29.0	40.0	2.0	41.0
16	30.964677872965815	37.0	29.0	40.0	2.0	41.0
17	31.26112162661529	38.0	30.0	40.0	2.0	41.0
18	31.29237834376578	38.0	30.0	40.0	2.0	41.0
19	30.992871710535596	38.0	30.0	40.0	2.0	41.0
20	30.884688705032115	38.0	29.0	40.0	2.0	41.0
21	30.816591114884783	38.0	30.0	40.0	2.0	41.0
22	30.090009957602614	37.0	27.0	40.0	2.0	41.0
23	30.169447367159094	37.0	27.0	40.0	2.0	41.0
24	29.806210948849515	37.0	27.0	40.0	2.0	41.0
25	29.325960570150322	37.0	26.0	40.0	2.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	183940.0
3	12.0
4	1466.0
5	7883.0
6	8861.0
7	7703.0
8	6626.0
9	5916.0
10	5774.0
11	5609.0
12	5200.0
13	5167.0
14	5270.0
15	5200.0
16	5642.0
17	6018.0
18	7089.0
19	8577.0
20	10862.0
21	13391.0
22	16180.0
23	18891.0
24	21765.0
25	24522.0
26	27033.0
27	30110.0
28	34222.0
29	39432.0
30	46152.0
31	55205.0
32	68677.0
33	86504.0
34	109892.0
35	141239.0
36	184305.0
37	238919.0
38	323261.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.816269262548595	17.544044807859887	11.689200782015451	25.950485147576067
2	16.35600166179718	20.458337660930155	37.93012549278157	25.255535184491094
3	19.762354963229797	24.17737496917713	28.753548509414443	27.306721558178626
4	14.031647872979692	15.969339536143407	34.10341841038416	35.89559418049274
5	14.362110591834398	36.49401891401015	32.98983206918324	16.154038424972224
6	33.17378512331418	33.706872477087515	17.733095252199572	15.386247147398738
7	29.178594600693952	31.240655589720447	20.276227820248806	19.30452198933679
8	27.108340611255166	33.14546139767693	20.429239417675984	19.31695857339192
9	27.660055845276197	15.821906717287378	18.790654689666027	37.72738274777039
10	16.615710632161374	27.832545249692092	31.947261491624836	23.6044826265217
11	35.520375011707834	22.744789591935533	21.593303233392653	20.141532162963983
12	24.063057166715566	24.787571373761537	28.54493443769889	22.60443702182401
13	29.07160049696394	21.59271074419334	24.49031292704312	24.8453758317996
14	23.516450405025065	20.83954958594679	25.253239410552546	30.390760598475598
15	25.015346526832726	27.415103239245582	22.906383929478196	24.663166304443497
16	24.70409567812924	26.814380388511804	24.298495265925897	24.183028667433057
17	23.930438198486602	26.941652363700396	25.036841543828597	24.0910678939844
18	23.853631394625733	26.63054703133165	25.80682296563236	23.70899860841026
19	24.905583721973475	25.851411095217543	26.152617784059974	23.090387398749016
20	25.43437143102195	25.117254330171946	25.76587729712405	23.682496941682054
21	25.815817273182823	25.00169291818465	25.27363200924559	23.908857799386936
22	24.982650701927213	24.328342377536526	26.571056518653176	24.11795040188309
23	25.34232546014399	24.915346145534095	25.458620473677883	24.28370792064403
24	24.8075364896371	25.194720484978706	25.66219635227763	24.335546673106567
25	24.314367696858184	25.058300427744314	26.2963555005334	24.330976374864097
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1008.0
1	1008.0
2	1055.0
3	1102.0
4	1102.0
5	1102.0
6	1423.5
7	1745.0
8	1745.0
9	1745.0
10	2162.5
11	2580.0
12	2580.0
13	2580.0
14	3663.5
15	4747.0
16	4747.0
17	4747.0
18	8137.5
19	11528.0
20	11528.0
21	11528.0
22	17970.0
23	24412.0
24	24412.0
25	24412.0
26	36272.0
27	48132.0
28	48132.0
29	48132.0
30	64181.5
31	80231.0
32	80231.0
33	80231.0
34	99258.0
35	118285.0
36	118285.0
37	118285.0
38	139641.5
39	160998.0
40	160998.0
41	160998.0
42	181057.0
43	201116.0
44	201116.0
45	201116.0
46	217994.5
47	234873.0
48	234873.0
49	234873.0
50	239799.5
51	244726.0
52	244726.0
53	244726.0
54	232154.5
55	219583.0
56	219583.0
57	219583.0
58	195775.0
59	171967.0
60	171967.0
61	171967.0
62	144528.5
63	117090.0
64	117090.0
65	117090.0
66	92799.5
67	68509.0
68	68509.0
69	68509.0
70	51490.5
71	34472.0
72	34472.0
73	34472.0
74	24787.0
75	15102.0
76	15102.0
77	15102.0
78	10829.0
79	6556.0
80	6556.0
81	6556.0
82	4502.5
83	2449.0
84	2449.0
85	2449.0
86	1703.5
87	958.0
88	958.0
89	958.0
90	624.0
91	290.0
92	290.0
93	290.0
94	168.5
95	47.0
96	47.0
97	47.0
98	28.0
99	9.0
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03853281918629743
2	0.05297557425466075
3	0.016473767499851907
4	0.01128340239715884
5	0.010042228133471368
6	5.641701198579419E-4
7	0.0036671057790766226
8	0.00174892737155962
9	0.006657207414323715
10	0.004062024862977182
11	0.011114151361201457
12	0.0087446368577981
13	0.008631802833826512
14	0.01574034634403658
15	0.007164960522195863
16	0.03238336487984587
17	0.04163575484551612
18	0.024879902285735243
19	0.0174892737155962
20	0.016868686583752464
21	0.024090064117934122
22	0.006149454306451567
23	0.01681226957176667
24	0.01303232976871846
25	0.47260530940499795
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1772515.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.03916342105267
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.44758063277972	50.0830901891706
2	11.191352140426543	14.109869529657928
3	4.048729239464388	7.656855125225694
4	1.8374285442258738	4.63319833095847
5	1.0030361925884614	3.161528123090724
6	0.6175922690515552	2.3359499985791827
7	0.3957074665762411	1.7461547356701281
8	0.27821546103583744	1.4030775931601334
9	0.19247774872188228	1.0920272630935541
>10	0.9371282252203832	10.089217596774963
>50	0.03783200001974664	1.634628529449474
>100	0.011637584990913705	1.2087391236862108
>500	7.328542277350583E-4	0.3422155178330927
>1k	5.496406708012937E-4	0.5034483436498316
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2327	0.1312823868909431	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	5.64170119857942E-5	0.0	0.0	0.0
3	0.0	5.64170119857942E-5	0.0	5.64170119857942E-5	0.0
4	0.0	5.64170119857942E-5	0.0	1.128340239715884E-4	0.0
5	0.0	5.64170119857942E-5	0.0	1.128340239715884E-4	0.0
6	0.0	5.64170119857942E-5	0.0	1.128340239715884E-4	5.64170119857942E-5
7	0.0	5.64170119857942E-5	0.0	1.128340239715884E-4	5.64170119857942E-5
8	0.0	5.64170119857942E-5	0.0	1.128340239715884E-4	1.128340239715884E-4
9	0.0	5.64170119857942E-5	0.0	1.128340239715884E-4	1.692510359573826E-4
10	0.0	5.64170119857942E-5	0.0	1.128340239715884E-4	1.692510359573826E-4
11	0.0	5.64170119857942E-5	0.0	1.128340239715884E-4	1.692510359573826E-4
12	0.0	5.64170119857942E-5	0.0	1.128340239715884E-4	1.692510359573826E-4
13	0.0	5.64170119857942E-5	0.0	1.692510359573826E-4	2.256680479431768E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1230	0.0	13.981358	1
CCCTACG	115	5.315087E-9	12.392796	1
TACGAGA	105	3.4873538E-6	10.851709	4
CTAGACA	150	2.1500455E-9	10.761278	4
TGCACCG	80	3.7877334E-4	10.682452	5
GTAGTAC	80	3.7887212E-4	10.682151	3
TACACCG	85	6.622614E-4	10.054072	5
GTATCAA	2540	0.0	10.024828	1
TCAACTC	275	0.0	10.013451	5
GTACGTG	95	1.6421212E-4	10.001204	1
AGACCGT	145	1.6279955E-7	9.822945	6
GATATAA	165	1.0197255E-8	9.789057	1
TAGACAG	205	4.1836756E-11	9.727111	5
CCGATCG	80	0.0045241183	9.497926	18
TCTCGCA	100	2.761376E-4	9.497925	18
TTACACT	205	4.274625E-10	9.263654	4
CACCGTG	145	1.7155144E-6	9.168599	7
ACCGTGC	115	1.114192E-4	9.084203	8
TAATCTG	115	1.1162488E-4	9.0826645	5
CTCGCAA	95	0.0017594901	9.035525	19
>>END_MODULE