FastQCFastQC Report
Mon 15 Aug 2016
SRR2921197_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921197_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1772515
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT44940.2535380518641591No Hit
GGTATCAACGCAGAGTACTTTTTTT31520.1778264217792233No Hit
TATCAACGCAGAGTACTTTTTTTTT26930.15193101327774378No Hit
GGTATCAACGCAGAGTACATGGGGG22980.12964629354335508No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13050.014.3638971
GGTTAAC604.0378078E-412.6868931
CTTATAC3350.012.4975381
GCCTAAC550.003085990412.08186157
TCGGACT907.4282234E-611.6158574
TTATACA4200.011.540432
AGGACCG1751.8189894E-1211.3982225
CCGCGGT600.00587068811.0847329
TATACAC4350.010.70552353
GTATCAA29300.010.5218941
GGACCGA1106.0998427E-610.3558816
CCTAGGT750.002638921610.1374752
CTTAATA1151.0214251E-59.9170952
TATAGGG1151.0214251E-59.9170952
CTATTAA1451.5796468E-79.8432791
GTGTAAG2800.09.515171
TAGGACA1606.591472E-89.5038844
TGACCCG900.00111526539.4985185
TGGTATC3850.09.3804572
GTATTAA1554.161775E-79.2082291
Produced by FastQC (version 0.11.5)