##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921197_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1772515
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.423693452523676	31.0	30.0	33.0	25.0	34.0
2	29.682911569154562	31.0	30.0	34.0	26.0	34.0
3	29.808122357215595	31.0	30.0	34.0	26.0	34.0
4	33.48430845437133	35.0	35.0	37.0	28.0	37.0
5	33.03214302840879	35.0	35.0	37.0	26.0	37.0
6	33.011861112599895	36.0	35.0	37.0	26.0	37.0
7	32.86549338087407	36.0	33.0	37.0	26.0	37.0
8	32.805417725660995	36.0	33.0	37.0	25.0	37.0
9	34.16161894257594	38.0	35.0	39.0	25.0	39.0
10	34.023085841304585	38.0	34.0	39.0	25.0	39.0
11	34.05945732476171	38.0	35.0	39.0	25.0	39.0
12	33.915254877955896	38.0	34.0	39.0	25.0	39.0
13	33.89410301182218	38.0	34.0	39.0	25.0	39.0
14	34.82734588987964	39.0	34.0	41.0	24.0	41.0
15	34.81908756766516	39.0	34.0	41.0	24.0	41.0
16	34.7977100334835	39.0	34.0	41.0	23.0	41.0
17	34.7778540661151	39.0	34.0	41.0	23.0	41.0
18	34.72474365520179	39.0	34.0	41.0	23.0	41.0
19	34.758189352417325	39.0	34.0	41.0	22.0	41.0
20	34.69468354287552	39.0	34.0	41.0	22.0	41.0
21	34.625325596680426	39.0	34.0	41.0	19.0	41.0
22	34.533035827623465	39.0	34.0	41.0	19.0	41.0
23	34.40404679226974	39.0	34.0	41.0	18.0	41.0
24	34.32549851482216	39.0	34.0	41.0	18.0	41.0
25	34.18754594460414	39.0	34.0	41.0	16.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	62256.0
3	5.0
4	976.0
5	8231.0
6	9181.0
7	7086.0
8	5517.0
9	4766.0
10	4081.0
11	3846.0
12	3524.0
13	3371.0
14	3332.0
15	3306.0
16	3334.0
17	3461.0
18	3892.0
19	4623.0
20	5767.0
21	7547.0
22	9167.0
23	11296.0
24	13269.0
25	15448.0
26	17483.0
27	19523.0
28	22742.0
29	27874.0
30	35115.0
31	44361.0
32	56526.0
33	72870.0
34	95455.0
35	126817.0
36	172628.0
37	261079.0
38	622760.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.71642657579226	18.525919027430053	9.113212161190615	31.644442235587068
2	15.33656978925425	21.10492717974178	34.672428724157484	28.88607430684649
3	18.580316279195774	24.321406217105988	27.31154781974603	29.786729683952206
4	13.919024569227078	16.524519834605776	32.60987002723642	36.94658556893072
5	15.096362342051735	37.152525111678024	31.889756445090768	15.861356101179473
6	33.680985160197984	34.47620627341727	16.646496993167133	15.196311573217608
7	29.756150126037813	31.6132049711027	19.665339715337346	18.965305187522144
8	27.792067259546947	34.17200900209142	18.744728422428047	19.291195315933578
9	27.644388628993493	15.625881519293566	18.577010653830616	38.15271919788232
10	17.384997892819847	28.486931815937954	30.332280025342577	23.79579026589962
11	35.93054029916259	22.553358823028287	21.32293660693683	20.193164270872295
12	24.321076528677942	24.937884764672962	27.97041712552581	22.770621581123287
13	29.437212585071432	21.105839780511047	24.499783072427856	24.957164561989664
14	23.496672128917396	20.530235335888907	25.149064126221454	30.824028408972243
15	25.145606139694994	27.457190017352538	22.429242554632282	24.96796128832019
16	25.20491017126248	26.659422595634148	23.690030488286496	24.44563674481688
17	23.838885099512044	26.93112943928318	25.013610727001822	24.216374734202954
18	23.904233670205773	26.37117125656072	26.055399183404504	23.669195889829002
19	25.170086449445723	25.62842704660868	25.852576134807652	23.348910369137947
20	25.37005746137507	24.8900561073515	25.83189233255947	23.90799409871396
21	25.591500981697546	24.748030962966304	25.490679515244523	24.169788540091623
22	25.49276807107753	24.510193868840787	25.368310176604965	24.62872788347672
23	25.100297266938266	24.679023357655176	25.532629122681712	24.688050252724853
24	24.796260157372632	25.077730029264067	25.580415248456823	24.545594564906477
25	24.593908643785134	25.05404096432251	25.758650368947904	24.59340002294445
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	685.0
1	685.0
2	713.0
3	741.0
4	741.0
5	741.0
6	1017.5
7	1294.0
8	1294.0
9	1294.0
10	1608.5
11	1923.0
12	1923.0
13	1923.0
14	2586.0
15	3249.0
16	3249.0
17	3249.0
18	6315.5
19	9382.0
20	9382.0
21	9382.0
22	15670.0
23	21958.0
24	21958.0
25	21958.0
26	33601.5
27	45245.0
28	45245.0
29	45245.0
30	60165.0
31	75085.0
32	75085.0
33	75085.0
34	93015.0
35	110945.0
36	110945.0
37	110945.0
38	131932.5
39	152920.0
40	152920.0
41	152920.0
42	172807.5
43	192695.0
44	192695.0
45	192695.0
46	212536.5
47	232378.0
48	232378.0
49	232378.0
50	242183.0
51	251988.0
52	251988.0
53	251988.0
54	240863.5
55	229739.0
56	229739.0
57	229739.0
58	205273.0
59	180807.0
60	180807.0
61	180807.0
62	153188.5
63	125570.0
64	125570.0
65	125570.0
66	99526.0
67	73482.0
68	73482.0
69	73482.0
70	54932.0
71	36382.0
72	36382.0
73	36382.0
74	25935.5
75	15489.0
76	15489.0
77	15489.0
78	11086.0
79	6683.0
80	6683.0
81	6683.0
82	4661.0
83	2639.0
84	2639.0
85	2639.0
86	1771.0
87	903.0
88	903.0
89	903.0
90	583.0
91	263.0
92	263.0
93	263.0
94	160.0
95	57.0
96	57.0
97	57.0
98	35.0
99	13.0
100	13.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.10527414436549197
2	0.0
3	0.040958750701686586
4	0.05585284186593626
5	0.06363838951997586
6	0.0029336846232612982
7	0.00174892737155962
8	0.0021438464554601796
9	3.9491908390055933E-4
10	2.2566804794317677E-4
11	0.0019181784075170025
12	1.692510359573826E-4
13	0.0019181784075170025
14	0.05427316553033402
15	0.024823485273749446
16	0.00174892737155962
17	9.026721917727071E-4
18	0.0019181784075170025
19	0.0019181784075170025
20	0.0011283402397158839
21	0.004231275898934564
22	0.0015796763356022373
23	0.0019181784075170025
24	0.0019181784075170025
25	0.17060504424504166
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1772515.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.941505259278124
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.93551921603537	34.92685100737977
2	14.890970425093641	14.873549556011382
3	6.068704717324903	9.092407456718627
4	3.058317512916488	6.109479206234447
5	1.693177130583632	4.227990728596596
6	1.002594382560291	3.0042643577734487
7	0.7026919476865824	2.4565445520729288
8	0.4790296247627813	1.9138768419552377
9	0.3531199851599204	1.587180923641834
>10	1.7032103813965145	14.988355616621318
>50	0.08130620276074635	2.7580246925647085
>100	0.028745685236708025	2.344952339149692
>500	0.0012495944915563348	0.40450842817049365
>1k	0.0013631939907887288	1.3120142931095142
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	4494	0.2535380518641591	No Hit
GGTATCAACGCAGAGTACTTTTTTT	3152	0.1778264217792233	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2693	0.15193101327774378	No Hit
GGTATCAACGCAGAGTACATGGGGG	2298	0.12964629354335508	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	5.64170119857942E-5	1.128340239715884E-4
2	0.0	0.0	0.0	5.64170119857942E-5	1.128340239715884E-4
3	0.0	0.0	0.0	1.128340239715884E-4	1.128340239715884E-4
4	0.0	0.0	0.0	1.128340239715884E-4	1.692510359573826E-4
5	0.0	0.0	0.0	1.128340239715884E-4	1.692510359573826E-4
6	0.0	0.0	0.0	1.128340239715884E-4	1.692510359573826E-4
7	0.0	0.0	0.0	1.128340239715884E-4	1.692510359573826E-4
8	0.0	0.0	0.0	1.692510359573826E-4	2.256680479431768E-4
9	0.0	0.0	0.0	1.692510359573826E-4	2.8208505992897097E-4
10	0.0	0.0	0.0	1.692510359573826E-4	2.8208505992897097E-4
11	0.0	5.64170119857942E-5	0.0	1.692510359573826E-4	3.385020719147652E-4
12	0.0	5.64170119857942E-5	0.0	1.692510359573826E-4	3.385020719147652E-4
13	0.0	5.64170119857942E-5	0.0	1.692510359573826E-4	3.385020719147652E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1305	0.0	14.363897	1
GGTTAAC	60	4.0378078E-4	12.686893	1
CTTATAC	335	0.0	12.497538	1
GCCTAAC	55	0.0030859904	12.0818615	7
TCGGACT	90	7.4282234E-6	11.615857	4
TTATACA	420	0.0	11.54043	2
AGGACCG	175	1.8189894E-12	11.398222	5
CCGCGGT	60	0.005870688	11.084732	9
TATACAC	435	0.0	10.7055235	3
GTATCAA	2930	0.0	10.521894	1
GGACCGA	110	6.0998427E-6	10.355881	6
CCTAGGT	75	0.0026389216	10.137475	2
CTTAATA	115	1.0214251E-5	9.917095	2
TATAGGG	115	1.0214251E-5	9.917095	2
CTATTAA	145	1.5796468E-7	9.843279	1
GTGTAAG	280	0.0	9.51517	1
TAGGACA	160	6.591472E-8	9.503884	4
TGACCCG	90	0.0011152653	9.498518	5
TGGTATC	385	0.0	9.380457	2
GTATTAA	155	4.161775E-7	9.208229	1
>>END_MODULE