FastQCFastQC Report
Mon 15 Aug 2016
SRR2921196_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921196_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2135756
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9300.012.6695091
GTATCAA21400.011.0562191
ACCGTCC1553.1468517E-1011.02827558
TACTAGC951.3598332E-511.0006642
AGCGGTC1106.0735965E-610.3598948
ACAGTCG750.002655694710.1299115
TAAGGTG1901.0004442E-109.9966235
TAATAGT1356.476548E-79.8482954
AGACCGT1651.0284566E-89.7843446
TATACTG2350.09.6988525
GTCCTAG2750.09.6748981
TAGACAG2201.6370905E-119.4967925
GACCGTC1502.6627458E-79.496577
GGACCGT1106.864971E-59.496576
GTCTTAA1951.6752892E-99.2584881
GTTACAC1554.280446E-79.19065953
CTTAGAC2201.6916601E-109.0653313
CTTATAC1804.240428E-88.9694023
TAGCACG850.007458728.9379484
TAAGCCT1701.694425E-78.9379484
Produced by FastQC (version 0.11.5)