##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921196_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2135756
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.549243452903795	31.0	28.0	31.0	16.0	34.0
2	27.310960147132914	31.0	27.0	31.0	10.0	34.0
3	27.19292981033414	31.0	27.0	31.0	10.0	34.0
4	31.10231880420797	35.0	32.0	37.0	17.0	37.0
5	31.02343385667651	35.0	32.0	37.0	16.0	37.0
6	31.328322149159362	35.0	32.0	37.0	17.0	37.0
7	31.214519355207244	35.0	32.0	37.0	16.0	37.0
8	30.930843223664127	35.0	32.0	37.0	10.0	37.0
9	32.604360235907095	37.0	32.0	39.0	15.0	39.0
10	31.982342552239114	37.0	32.0	39.0	10.0	39.0
11	32.46091079692624	37.0	32.0	39.0	11.0	39.0
12	32.150787355859	37.0	32.0	39.0	10.0	39.0
13	32.16574833454758	37.0	32.0	39.0	10.0	39.0
14	33.32806041514105	38.0	32.0	40.0	10.0	41.0
15	32.969666010536784	38.0	32.0	40.0	10.0	41.0
16	32.897992560947976	38.0	32.0	40.0	8.0	41.0
17	33.22295992613388	38.0	32.0	40.0	8.0	41.0
18	33.272788651887204	38.0	32.0	40.0	2.0	41.0
19	32.960523580408996	38.0	32.0	40.0	2.0	41.0
20	32.84296099367156	38.0	32.0	40.0	2.0	41.0
21	32.8040970035903	38.0	32.0	40.0	2.0	41.0
22	32.0100058246354	38.0	31.0	40.0	2.0	41.0
23	32.144561925613225	38.0	31.0	40.0	2.0	41.0
24	31.778200318763005	38.0	31.0	40.0	2.0	41.0
25	31.278308945403875	38.0	30.0	40.0	2.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	142870.0
3	8.0
4	1108.0
5	6580.0
6	8112.0
7	7097.0
8	5972.0
9	5475.0
10	5340.0
11	5235.0
12	5097.0
13	5041.0
14	5049.0
15	5236.0
16	5692.0
17	6164.0
18	7195.0
19	8756.0
20	11071.0
21	14009.0
22	17182.0
23	20351.0
24	23705.0
25	26951.0
26	30594.0
27	34454.0
28	38831.0
29	46001.0
30	54727.0
31	65567.0
32	81831.0
33	104924.0
34	135530.0
35	178072.0
36	238739.0
37	321015.0
38	456175.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.856847770338156	17.27157119671751	12.066989388584824	25.80459164435951
2	16.402258432345878	19.17718631036205	38.85451831696945	25.56603694032263
3	19.679021977502224	22.268078604072002	28.949789993589054	29.103109424836717
4	14.294584678637237	15.263390798004227	34.21394839183991	36.22807613151863
5	14.599410363222248	35.58691148705346	34.16034823783266	15.653329911891634
6	34.676017337259516	33.66962472614968	16.69200518600752	14.962352750583285
7	30.887301778311983	30.347098367402936	19.627780792694775	19.137819061590307
8	26.6895223254712	33.692680976269386	20.137171410885873	19.48062528737354
9	27.124696398360935	16.079613749882352	18.706122041888715	38.089567809867994
10	16.137184115523464	27.440007117204434	32.3394063689615	24.0834023983106
11	36.30488154936641	22.27090124189253	21.579785074531816	19.844432134209246
12	24.132957788688238	24.003156073451287	28.548463755493277	23.315422382367196
13	28.9583575262876	20.569224314574587	25.021773658836267	25.450644500301557
14	23.255468723658527	20.341141960161394	25.14306345929786	31.260325856882222
15	25.083231177111266	27.23502049523838	22.537925790099166	25.14382253755119
16	24.9739706157173	26.699989274441744	24.100704093088478	24.22533601675248
17	24.028355280384055	26.712073711737865	25.147465163653195	24.11210584422489
18	24.016985221527225	26.46623281012032	25.73302274000266	23.78375922834979
19	25.04070660198567	25.546981061654407	26.1010216806851	23.311290655674828
20	25.40186812600157	25.214128299964127	25.55963701448252	23.824366559551784
21	25.815284840389907	24.837280226444854	25.281627675427792	24.065807257737443
22	24.53398000972075	24.470392454151874	26.673387531032883	24.322240005094496
23	25.01728010250051	24.780790894837697	25.42783473291237	24.774094269749426
24	24.435246447066692	25.278659829200713	25.79423260901836	24.49186111471424
25	24.326651123141975	24.917483830782576	26.27194393793128	24.483921108144166
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	614.0
1	614.0
2	642.5
3	671.0
4	671.0
5	671.0
6	872.0
7	1073.0
8	1073.0
9	1073.0
10	1396.5
11	1720.0
12	1720.0
13	1720.0
14	2441.5
15	3163.0
16	3163.0
17	3163.0
18	6008.5
19	8854.0
20	8854.0
21	8854.0
22	15999.5
23	23145.0
24	23145.0
25	23145.0
26	37396.5
27	51648.0
28	51648.0
29	51648.0
30	71502.0
31	91356.0
32	91356.0
33	91356.0
34	116391.5
35	141427.0
36	141427.0
37	141427.0
38	170362.0
39	199297.0
40	199297.0
41	199297.0
42	224776.5
43	250256.0
44	250256.0
45	250256.0
46	270163.5
47	290071.0
48	290071.0
49	290071.0
50	294881.5
51	299692.0
52	299692.0
53	299692.0
54	283457.0
55	267222.0
56	267222.0
57	267222.0
58	237997.0
59	208772.0
60	208772.0
61	208772.0
62	176070.5
63	143369.0
64	143369.0
65	143369.0
66	112974.5
67	82580.0
68	82580.0
69	82580.0
70	61760.5
71	40941.0
72	40941.0
73	40941.0
74	29381.5
75	17822.0
76	17822.0
77	17822.0
78	12704.0
79	7586.0
80	7586.0
81	7586.0
82	5293.5
83	3001.0
84	3001.0
85	3001.0
86	2076.0
87	1151.0
88	1151.0
89	1151.0
90	717.0
91	283.0
92	283.0
93	283.0
94	159.0
95	35.0
96	35.0
97	35.0
98	21.0
99	7.0
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.037036065917642275
2	0.055156113338789635
3	0.016153530646759274
4	0.011284060538750681
5	0.009551652904170701
6	4.213964516545898E-4
7	0.0036989244089680657
8	0.001591942150695117
9	0.006789165054435057
10	0.004026677204699414
11	0.010534911291364744
12	0.008942969140669627
13	0.006929630538319921
14	0.015919421506951168
15	0.006461412258703709
16	0.031323802906324506
17	0.03956444462756981
18	0.022849052045271087
19	0.01652810527045224
20	0.016574927098413864
21	0.02294269570119433
22	0.005524975699471288
23	0.016481283442490623
24	0.012501428065752829
25	0.4734623243479124
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2135756.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.93221421116739
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.57065845129036	37.16822765926708
2	13.094037458091456	13.600047163253103
3	5.746774707581746	8.953282054123623
4	3.0855368648625996	6.409550456899934
5	1.7967744981319687	4.665523906307609
6	1.1596386756018064	3.613356246534447
7	0.7599309831281402	2.762542902105953
8	0.5386089525050698	2.2376924398036615
9	0.4100105514891018	1.9163480209893817
>10	1.7706932403250366	15.16142258812442
>50	0.050810457298669794	1.7682421696678337
>100	0.015412198576720158	1.218375708460844
>500	9.274675976217568E-4	0.38281896443929103
>1k	1.854935195243509E-4	0.14256972002268486
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.682182796162108E-5
2	0.0	0.0	0.0	0.0	9.364365592324216E-5
3	0.0	0.0	0.0	0.0	9.364365592324216E-5
4	0.0	0.0	0.0	0.0	9.364365592324216E-5
5	0.0	0.0	0.0	0.0	9.364365592324216E-5
6	0.0	0.0	0.0	0.0	1.4046548388486324E-4
7	0.0	0.0	0.0	0.0	1.8728731184648433E-4
8	0.0	0.0	0.0	0.0	2.3410913980810542E-4
9	0.0	0.0	0.0	0.0	3.2775279573134757E-4
10	0.0	0.0	0.0	0.0	3.2775279573134757E-4
11	0.0	0.0	0.0	0.0	3.2775279573134757E-4
12	0.0	0.0	0.0	0.0	3.2775279573134757E-4
13	0.0	0.0	0.0	0.0	3.2775279573134757E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	930	0.0	12.669509	1
GTATCAA	2140	0.0	11.056219	1
ACCGTCC	155	3.1468517E-10	11.0282755	8
TACTAGC	95	1.3598332E-5	11.000664	2
AGCGGTC	110	6.0735965E-6	10.359894	8
ACAGTCG	75	0.0026556947	10.129911	5
TAAGGTG	190	1.0004442E-10	9.996623	5
TAATAGT	135	6.476548E-7	9.848295	4
AGACCGT	165	1.0284566E-8	9.784344	6
TATACTG	235	0.0	9.698852	5
GTCCTAG	275	0.0	9.674898	1
TAGACAG	220	1.6370905E-11	9.496792	5
GACCGTC	150	2.6627458E-7	9.49657	7
GGACCGT	110	6.864971E-5	9.49657	6
GTCTTAA	195	1.6752892E-9	9.258488	1
GTTACAC	155	4.280446E-7	9.1906595	3
CTTAGAC	220	1.6916601E-10	9.065331	3
CTTATAC	180	4.240428E-8	8.969402	3
TAGCACG	85	0.00745872	8.937948	4
TAAGCCT	170	1.694425E-7	8.937948	4
>>END_MODULE