FastQCFastQC Report
Mon 15 Aug 2016
SRR2921196_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921196_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2135756
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT31400.1470205397994902No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10850.013.2396611
ATTTGCG1001.9438558E-611.39346816
CCTAGAC1201.269982E-711.0871073
ACGATAC600.00586200611.0871073
CGATACT600.00586200611.0871074
CTCCTAG3550.010.9871521
GTATCAA24750.010.4165461
TTGCGCG1201.5354781E-610.2857718
GACTGAT1701.5770638E-910.0521187
AGGTCGT856.635647E-410.0521186
TTCGCAG1054.104549E-59.95273710
CTACACG1151.0225696E-59.9164184
CTTATAC2400.09.9096531
TATACAC2900.09.8309323
GTGTACG1002.7175611E-49.5132671
TGCGCGC1201.6812995E-59.50925119
CTAGACA1106.812484E-59.5032344
TAGACAC1804.1836756E-99.4998945
ACCGACC1106.849252E-59.498568
AGTTCGC1002.7601194E-49.4985598
Produced by FastQC (version 0.11.5)