##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921196_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2135756
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.83775674749363	31.0	30.0	33.0	26.0	34.0
2	30.083900501742708	31.0	30.0	34.0	26.0	34.0
3	30.21155834280695	31.0	30.0	34.0	26.0	34.0
4	33.917764950677885	35.0	35.0	37.0	30.0	37.0
5	33.507446075300734	35.0	35.0	37.0	28.0	37.0
6	33.49828163891381	37.0	35.0	37.0	28.0	37.0
7	33.36138866050241	37.0	35.0	37.0	28.0	37.0
8	33.31045166208125	37.0	35.0	37.0	27.0	37.0
9	34.7226195314446	39.0	35.0	39.0	27.0	39.0
10	34.59967571201954	38.0	35.0	39.0	27.0	39.0
11	34.63828826888465	39.0	35.0	39.0	27.0	39.0
12	34.50519628646718	38.0	35.0	39.0	27.0	39.0
13	34.48420044237263	38.0	35.0	39.0	27.0	39.0
14	35.47714579755365	39.0	36.0	41.0	27.0	41.0
15	35.46557659208261	39.0	36.0	41.0	27.0	41.0
16	35.459882589584204	39.0	36.0	41.0	27.0	41.0
17	35.426819355769105	39.0	36.0	41.0	27.0	41.0
18	35.38342020343148	39.0	35.0	41.0	27.0	41.0
19	35.42011353356844	39.0	35.0	41.0	26.0	41.0
20	35.35362700608122	39.0	35.0	41.0	26.0	41.0
21	35.28888505990385	39.0	35.0	41.0	26.0	41.0
22	35.20751761905386	39.0	35.0	41.0	25.0	41.0
23	35.08177666362637	39.0	35.0	41.0	25.0	41.0
24	35.00918691086435	39.0	35.0	41.0	25.0	41.0
25	34.87785589739652	39.0	35.0	41.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	57427.0
3	7.0
4	955.0
5	7969.0
6	8749.0
7	6777.0
8	5311.0
9	4540.0
10	4169.0
11	3828.0
12	3585.0
13	3402.0
14	3411.0
15	3386.0
16	3460.0
17	3787.0
18	4091.0
19	4820.0
20	6100.0
21	7853.0
22	9885.0
23	12508.0
24	14943.0
25	17332.0
26	20061.0
27	22489.0
28	26176.0
29	32141.0
30	40644.0
31	52879.0
32	67541.0
33	87450.0
34	116025.0
35	152385.0
36	210378.0
37	320125.0
38	789167.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.78755510143821	18.278936896696372	9.508311206829884	31.425196795035536
2	15.255862561079075	20.46741294417527	35.10700660562349	29.169717889122165
3	19.001833346292603	22.67246055307664	27.239759913138545	31.085946187492215
4	13.949886510303083	15.810040031763062	32.505276275382926	37.73479718255093
5	15.465199903485981	36.48126761322242	32.05722437869102	15.99630810460058
6	35.04880362522486	34.04852362361416	15.979101953362406	14.923570797798568
7	30.90259032762684	30.760626345836805	18.854942495691215	19.48184083084514
8	27.92007944857966	34.4802528045564	18.264979367623393	19.334688379240546
9	27.190137824732787	15.974437154806074	18.32891959568415	38.50650542477699
10	17.086221459754768	27.991774341263703	30.509056277964337	24.412947921017196
11	36.62828482225298	22.221842440905508	21.179998239479485	19.969874497362028
12	24.406111934134795	24.2449512022909	27.87565620791888	23.473280655655422
13	29.155940536111437	20.402902961488937	24.756736509422918	25.684419992976704
14	23.33360972796678	20.167954244039596	24.90905913861378	31.58937688937984
15	25.180633740153425	27.437829585155903	22.02748143083278	25.354055243857893
16	25.499378673761118	26.68982476446257	23.281426606247724	24.529369955528583
17	24.13211059690339	26.726789015224583	25.016340817958604	24.12475956991342
18	24.14928305477102	26.354620063978185	25.599280432714277	23.89681644853652
19	25.236675626917087	25.50201828040917	25.63705413935944	23.6242519533143
20	25.40116942197517	25.186163587975408	25.480157845746426	23.932509144303
21	25.69089838915634	24.639197898097436	25.30149761962625	24.368406093119976
22	25.356688685411626	24.703805116314783	25.057028986457254	24.88247721181633
23	24.952112683969936	24.756208963344374	25.253272514530074	25.03840583815562
24	24.570777068155	25.32359110744854	25.35898900604948	24.746642818346977
25	24.825548866249363	24.94368648461561	25.336603104969896	24.894161544165133
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	461.0
1	461.0
2	455.0
3	449.0
4	449.0
5	449.0
6	631.5
7	814.0
8	814.0
9	814.0
10	1074.5
11	1335.0
12	1335.0
13	1335.0
14	1769.5
15	2204.0
16	2204.0
17	2204.0
18	4697.5
19	7191.0
20	7191.0
21	7191.0
22	13767.0
23	20343.0
24	20343.0
25	20343.0
26	33877.0
27	47411.0
28	47411.0
29	47411.0
30	64995.0
31	82579.0
32	82579.0
33	82579.0
34	105797.5
35	129016.0
36	129016.0
37	129016.0
38	158133.5
39	187251.0
40	187251.0
41	187251.0
42	212867.0
43	238483.0
44	238483.0
45	238483.0
46	261989.5
47	285496.0
48	285496.0
49	285496.0
50	296053.0
51	306610.0
52	306610.0
53	306610.0
54	292592.5
55	278575.0
56	278575.0
57	278575.0
58	250513.5
59	222452.0
60	222452.0
61	222452.0
62	189013.5
63	155575.0
64	155575.0
65	155575.0
66	123246.0
67	90917.0
68	90917.0
69	90917.0
70	68093.5
71	45270.0
72	45270.0
73	45270.0
74	32399.5
75	19529.0
76	19529.0
77	19529.0
78	14022.0
79	8515.0
80	8515.0
81	8515.0
82	6009.5
83	3504.0
84	3504.0
85	3504.0
86	2410.5
87	1317.0
88	1317.0
89	1317.0
90	846.5
91	376.0
92	376.0
93	376.0
94	225.5
95	75.0
96	75.0
97	75.0
98	41.5
99	8.0
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.10258662506391181
2	0.0
3	0.040360415702917374
4	0.05623301538190692
5	0.06325628957615008
6	0.0025283787099275386
7	1.4046548388486324E-4
8	0.001638763978656738
9	0.0
10	0.0
11	0.001123723871078906
12	0.0
13	2.809309677697265E-4
14	0.052768200112746966
15	0.02322362666896406
16	9.364365592324216E-5
17	0.0
18	0.0013110111829253903
19	0.0012641893549637693
20	0.0
21	0.00781924526959072
22	0.0
23	6.08683763501074E-4
24	0.0016855858066183592
25	0.16373593238178893
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2135756.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.4591638773264
#Duplication Level	Percentage of deduplicated	Percentage of total
1	62.02358024825505	25.09422197522656
2	15.641713267558705	12.657012808286163
3	7.54244245272471	9.154827456902783
4	4.289405641876938	6.941830632041099
5	2.5879137957284764	5.235241418088612
6	1.809633655750404	4.392975878155853
7	1.2264011885840216	3.473341666778816
8	0.892781339522597	2.8896949217890984
9	0.6631713714869402	2.4148223277967276
>10	3.1699858511406767	21.402690774943988
>50	0.11660597241689259	3.1438005536533518
>100	0.034495491179156394	2.276971524255764
>500	8.180041517230118E-4	0.23942421290313454
>1k	0.0010517196236438599	0.6831438491779942
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3140	0.1470205397994902	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.3410913980810542E-4
2	0.0	0.0	0.0	0.0	2.809309677697265E-4
3	0.0	0.0	0.0	0.0	2.809309677697265E-4
4	0.0	0.0	0.0	0.0	2.809309677697265E-4
5	0.0	0.0	0.0	0.0	2.809309677697265E-4
6	0.0	0.0	0.0	0.0	4.2139645165458975E-4
7	0.0	0.0	0.0	0.0	4.2139645165458975E-4
8	0.0	0.0	0.0	0.0	4.2139645165458975E-4
9	0.0	0.0	0.0	0.0	4.2139645165458975E-4
10	0.0	0.0	0.0	0.0	4.2139645165458975E-4
11	0.0	0.0	0.0	0.0	4.2139645165458975E-4
12	0.0	0.0	0.0	0.0	4.2139645165458975E-4
13	0.0	0.0	0.0	0.0	4.2139645165458975E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1085	0.0	13.239661	1
ATTTGCG	100	1.9438558E-6	11.393468	16
CCTAGAC	120	1.269982E-7	11.087107	3
ACGATAC	60	0.005862006	11.087107	3
CGATACT	60	0.005862006	11.087107	4
CTCCTAG	355	0.0	10.987152	1
GTATCAA	2475	0.0	10.416546	1
TTGCGCG	120	1.5354781E-6	10.28577	18
GACTGAT	170	1.5770638E-9	10.052118	7
AGGTCGT	85	6.635647E-4	10.052118	6
TTCGCAG	105	4.104549E-5	9.952737	10
CTACACG	115	1.0225696E-5	9.916418	4
CTTATAC	240	0.0	9.909653	1
TATACAC	290	0.0	9.830932	3
GTGTACG	100	2.7175611E-4	9.513267	1
TGCGCGC	120	1.6812995E-5	9.509251	19
CTAGACA	110	6.812484E-5	9.503234	4
TAGACAC	180	4.1836756E-9	9.499894	5
ACCGACC	110	6.849252E-5	9.49856	8
AGTTCGC	100	2.7601194E-4	9.498559	8
>>END_MODULE