FastQCFastQC Report
Mon 15 Aug 2016
SRR2921195_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921195_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1970981
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTCG508.7390916E-515.195259
GGTATCA9500.013.4009121
GATTCGC550.003073802212.08835810
TTGCGCG752.0798927E-411.39672718
TATGCGC600.005885841311.08099610
CTAGAAC1252.2519271E-710.6361353
GTATTTA1701.382432E-1010.618371
CCCTAGA3350.010.4932531
TAGGACA2500.010.2560134
TCTATAC1409.7419615E-810.1748743
GTATAAT1502.442539E-810.1340241
GTCTTAT1502.442539E-810.1340241
TACGATT750.002656102410.1296527
GTATCAA19250.010.1175721
TGGACCG1901.0004442E-109.9968765
CCTAGAC1151.0310281E-59.9094423
GTTCTAG1951.6370905E-109.7442541
GTTCTAA1106.8320864E-59.5006471
TCTTATA1106.8415786E-59.4994412
ATTTGCG1002.760117E-49.49847816
Produced by FastQC (version 0.11.5)