##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921195_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1970981
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.8968148348462	30.0	26.0	31.0	2.0	34.0
2	25.52366106015228	31.0	25.0	31.0	2.0	34.0
3	25.41256105462204	30.0	25.0	31.0	2.0	34.0
4	29.097465678258693	35.0	28.0	37.0	2.0	37.0
5	28.830566098810692	35.0	28.0	37.0	2.0	37.0
6	28.925370158312028	35.0	28.0	37.0	2.0	37.0
7	28.856043767037836	35.0	28.0	37.0	2.0	37.0
8	28.616011011775356	35.0	28.0	37.0	2.0	37.0
9	30.172032607112904	37.0	29.0	39.0	2.0	39.0
10	29.548820105318114	35.0	27.0	39.0	2.0	39.0
11	29.952059913312205	37.0	28.0	39.0	2.0	39.0
12	29.665789776765987	37.0	27.0	39.0	2.0	39.0
13	29.6407697486683	37.0	27.0	39.0	2.0	39.0
14	30.666864368555558	37.0	28.0	40.0	2.0	41.0
15	30.311052212071043	37.0	27.0	40.0	2.0	41.0
16	30.241832366724996	37.0	27.0	40.0	2.0	41.0
17	30.513234272679444	37.0	28.0	40.0	2.0	41.0
18	30.547461391053492	37.0	29.0	40.0	2.0	41.0
19	30.23848479513501	37.0	27.0	40.0	2.0	41.0
20	30.114049805655153	37.0	27.0	40.0	2.0	41.0
21	30.038615795890472	37.0	27.0	40.0	2.0	41.0
22	29.316572813233613	37.0	25.0	40.0	2.0	41.0
23	29.393134180390373	37.0	26.0	40.0	2.0	41.0
24	29.038731474326745	37.0	24.0	40.0	2.0	41.0
25	28.559892256698568	37.0	21.0	40.0	2.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	234980.0
3	12.0
4	1971.0
5	10072.0
6	11196.0
7	9260.0
8	7970.0
9	7478.0
10	7084.0
11	6720.0
12	6301.0
13	6103.0
14	6021.0
15	6210.0
16	6639.0
17	7226.0
18	8212.0
19	10035.0
20	12557.0
21	15582.0
22	18637.0
23	21866.0
24	24744.0
25	27706.0
26	30897.0
27	34224.0
28	37479.0
29	43759.0
30	50834.0
31	60222.0
32	74998.0
33	93657.0
34	119207.0
35	152465.0
36	199353.0
37	256586.0
38	342718.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.174657497745244	17.364241307956892	12.254508117885843	26.20659307641202
2	16.212242892257148	18.76796416456926	38.06077890485478	26.959014038318813
3	19.583744573532286	22.131818447072433	29.171726295438344	29.112710683956937
4	13.975523235369783	14.85802228269501	33.42495586743638	37.741498614498816
5	14.78012281419254	35.39513756468244	33.85701716374515	15.967722457379862
6	35.032996627530046	32.66678809655604	16.533677260663467	15.766538015250445
7	30.45513078756673	30.18510268881418	19.90424708230631	19.455519441312777
8	27.87095929666191	33.05005114300559	19.67361832705546	19.405371233277048
9	27.128825502878957	15.923620850271863	18.390523489333546	38.557030157515634
10	16.58334673656983	27.08683318475613	31.843045648294677	24.486774430379363
11	36.48611278135591	22.14247331879079	21.19700482400525	20.174409075848057
12	24.520701280091902	23.546069709904017	28.153497368575948	23.779731641428135
13	29.137317779818655	20.399527102794256	24.547931582125297	25.915223535261795
14	23.435054773082943	19.769104611818484	24.82285266578575	31.972987949312824
15	24.999720931459752	26.894190401868844	22.456645433779066	25.649443232892338
16	25.18637580081072	26.17214125227688	23.817073845368352	24.824409101544042
17	24.280948269692555	26.275975167990644	24.74496231091377	24.69811425140303
18	24.372316406830823	26.09174444149545	25.297951474190565	24.237987677483165
19	25.298341103201132	25.31123027107341	25.559169145970838	23.831259479754618
20	25.511451977607546	24.988075085351944	25.319080269391435	24.181392667649078
21	25.959434387395103	24.43132223922831	24.90566018585806	24.703583187518525
22	25.0308875156594	23.961405700351268	26.293584377589283	24.714122406400048
23	25.291196562373457	24.27996277393079	25.351024173602575	25.077816490093173
24	24.884358629312324	24.791246775336067	25.334745994921725	24.989648600429888
25	24.560519866903142	24.621185279817944	25.7284565138595	25.08983833941942
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	692.0
1	692.0
2	717.0
3	742.0
4	742.0
5	742.0
6	942.0
7	1142.0
8	1142.0
9	1142.0
10	1465.0
11	1788.0
12	1788.0
13	1788.0
14	2571.0
15	3354.0
16	3354.0
17	3354.0
18	6058.5
19	8763.0
20	8763.0
21	8763.0
22	14551.5
23	20340.0
24	20340.0
25	20340.0
26	31957.5
27	43575.0
28	43575.0
29	43575.0
30	60084.5
31	76594.0
32	76594.0
33	76594.0
34	97828.0
35	119062.0
36	119062.0
37	119062.0
38	144435.0
39	169808.0
40	169808.0
41	169808.0
42	193979.0
43	218150.0
44	218150.0
45	218150.0
46	240122.0
47	262094.0
48	262094.0
49	262094.0
50	270496.5
51	278899.0
52	278899.0
53	278899.0
54	267318.0
55	255737.0
56	255737.0
57	255737.0
58	229905.0
59	204073.0
60	204073.0
61	204073.0
62	174654.0
63	145235.0
64	145235.0
65	145235.0
66	115800.0
67	86365.0
68	86365.0
69	86365.0
70	64985.0
71	43605.0
72	43605.0
73	43605.0
74	31227.0
75	18849.0
76	18849.0
77	18849.0
78	13335.5
79	7822.0
80	7822.0
81	7822.0
82	5387.0
83	2952.0
84	2952.0
85	2952.0
86	1975.0
87	998.0
88	998.0
89	998.0
90	642.5
91	287.0
92	287.0
93	287.0
94	164.0
95	41.0
96	41.0
97	41.0
98	27.5
99	14.0
100	14.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.035616781693988936
2	0.055150201853797674
3	0.016032625377920946
4	0.010857537439478107
5	0.010096495095589455
6	0.0011161954377033568
7	0.003805211719443262
8	0.0018772377815920093
9	0.006544964157442411
10	0.003247114000591584
11	0.01075606512695962
12	0.009386188907960046
13	0.007661159595145768
14	0.015474527659069265
15	0.007052325720034845
16	0.032166723068360374
17	0.03967567419472841
18	0.022425381066585624
19	0.016945876190587326
20	0.016083361534180187
21	0.022577589535363353
22	0.006088338751109219
23	0.016184833846698676
24	0.011821524408403734
25	0.4770213411494073
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1970981.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.39554823734605
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.06699377630075	45.00369352571219
2	11.197385113182383	13.07754885017965
3	4.653975961632186	8.15314433288824
4	2.3678214255158063	5.530809210845191
5	1.3469221384696564	3.932712835447706
6	0.8319718515140129	2.9150071432320392
7	0.5623901598635388	2.2988757195964027
8	0.38471842986290544	1.7972674903084265
9	0.283975243619726	1.4924601033307023
>10	1.241303514126491	12.016321385942733
>50	0.04660378354650376	1.84238100522761
>100	0.014870617390126847	1.4693860992962735
>500	8.899874799512835E-4	0.3377615229980231
>1k	1.7799749599025478E-4	0.13263077499476034
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	1.5220846877773048E-4
13	0.0	0.0	0.0	0.0	1.5220846877773048E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGATTCG	50	8.7390916E-5	15.19525	9
GGTATCA	950	0.0	13.400912	1
GATTCGC	55	0.0030738022	12.088358	10
TTGCGCG	75	2.0798927E-4	11.396727	18
TATGCGC	60	0.0058858413	11.080996	10
CTAGAAC	125	2.2519271E-7	10.636135	3
GTATTTA	170	1.382432E-10	10.61837	1
CCCTAGA	335	0.0	10.493253	1
TAGGACA	250	0.0	10.256013	4
TCTATAC	140	9.7419615E-8	10.174874	3
GTATAAT	150	2.442539E-8	10.134024	1
GTCTTAT	150	2.442539E-8	10.134024	1
TACGATT	75	0.0026561024	10.129652	7
GTATCAA	1925	0.0	10.117572	1
TGGACCG	190	1.0004442E-10	9.996876	5
CCTAGAC	115	1.0310281E-5	9.909442	3
GTTCTAG	195	1.6370905E-10	9.744254	1
GTTCTAA	110	6.8320864E-5	9.500647	1
TCTTATA	110	6.8415786E-5	9.499441	2
ATTTGCG	100	2.760117E-4	9.498478	16
>>END_MODULE