FastQCFastQC Report
Mon 15 Aug 2016
SRR2921195_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921195_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1970981
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT29310.14870767399584267No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGAC400.005306879514.2390027
TACACCG905.4097836E-712.666215
GGTATCA9200.012.620121
TAGCACT1501.7462298E-1011.4074094
GTATCAA21450.011.3136931
CTAAGAC1201.2638884E-711.0908173
TAAGACC1252.2207314E-710.64691454
CTTATAC2250.010.5742371
ATAGCAC2700.010.2105943
TACCGTG856.641585E-410.051067
ACAACCG951.6449284E-49.9996395
GTATTAC1054.0267154E-59.9699951
CCCTAAT800.0044509499.5168131
CCCTAGA4100.09.5168121
CCTAGAC1304.2026077E-69.5064153
TCTTATA1106.7868634E-59.5064152
GTAGGAC1201.6870355E-59.5064153
TATTAGG1502.6224552E-79.5064152
TTAGGGT1701.6470949E-89.5061744
CTACACG800.00449221269.5061744
Produced by FastQC (version 0.11.5)