##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921195_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1970981
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.254741166962035	31.0	30.0	33.0	25.0	34.0
2	29.559643142171335	31.0	30.0	34.0	25.0	34.0
3	29.698873809539514	31.0	30.0	34.0	26.0	34.0
4	33.35552397511696	35.0	35.0	37.0	28.0	37.0
5	32.863423340965745	35.0	33.0	37.0	26.0	37.0
6	32.79450537574944	36.0	33.0	37.0	26.0	37.0
7	32.64502600481689	36.0	33.0	37.0	25.0	37.0
8	32.579141047021764	36.0	33.0	37.0	25.0	37.0
9	33.94984172856055	38.0	34.0	39.0	25.0	39.0
10	33.80580482510993	38.0	34.0	39.0	23.0	39.0
11	33.82651075784089	38.0	34.0	39.0	23.0	39.0
12	33.69139783691472	38.0	34.0	39.0	22.0	39.0
13	33.661333620161734	38.0	34.0	39.0	22.0	39.0
14	34.613586330867726	39.0	34.0	41.0	19.0	41.0
15	34.58129479685497	39.0	34.0	41.0	19.0	41.0
16	34.55660404641141	39.0	34.0	41.0	19.0	41.0
17	34.521817815595384	39.0	34.0	41.0	18.0	41.0
18	34.46406231211767	39.0	34.0	41.0	18.0	41.0
19	34.489881942038004	39.0	34.0	41.0	18.0	41.0
20	34.42293253968455	39.0	34.0	41.0	18.0	41.0
21	34.35085574137955	39.0	34.0	41.0	18.0	41.0
22	34.26519129306675	39.0	34.0	41.0	17.0	41.0
23	34.13385872314345	39.0	34.0	41.0	11.0	41.0
24	34.04686803170604	39.0	34.0	41.0	8.0	41.0
25	33.907094994827446	39.0	34.0	41.0	2.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	75963.0
3	3.0
4	1173.0
5	10420.0
6	11483.0
7	8520.0
8	6620.0
9	5781.0
10	5130.0
11	4487.0
12	4384.0
13	4023.0
14	3865.0
15	3864.0
16	3946.0
17	4171.0
18	4502.0
19	5444.0
20	6635.0
21	8570.0
22	10692.0
23	12916.0
24	15370.0
25	17818.0
26	19714.0
27	21960.0
28	25598.0
29	30769.0
30	38659.0
31	49156.0
32	61856.0
33	79076.0
34	104137.0
35	137796.0
36	186845.0
37	285174.0
38	694461.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.33669226538221	18.29085023253168	9.455966772626828	31.91649072945928
2	15.263718929812109	20.019776953709854	34.61661984565046	30.09988427082757
3	18.759028573690927	22.62135468957949	26.95750644372413	31.66211029300545
4	13.82855970926251	15.665060990808103	31.899443978258653	38.60693532167074
5	15.660156537589584	36.493365050431706	31.783528264859772	16.062950147118933
6	35.69610259267321	33.56562207768024	15.764226417085652	14.974048912560894
7	31.29382373295504	30.62572426487749	18.513225000558105	19.567227001609364
8	28.6288225471487	34.15469249390742	17.683179600160134	19.533305358783746
9	27.180184476757756	15.94333783194147	18.07126404123837	38.805213650062406
10	17.461659955118797	27.763281330464373	29.95716346327032	24.81789525114651
11	36.92967701507229	22.13604893357384	20.837513033815615	20.09676101753825
12	24.759092046042046	23.81585616502645	27.450695871751172	23.97435591718033
13	29.323901485150778	20.281636258562667	24.490096204242604	25.904366052043954
14	23.50551472921347	19.651474190559597	24.592722334336013	32.25028874589093
15	25.15734345582153	27.094842504300875	21.726134593231265	26.021679446646335
16	25.650421251043298	26.273279162216532	22.971005852611604	25.105293734128566
17	24.375868223795496	26.421908311508297	24.6261500635725	24.576073401123704
18	24.559269745588132	26.03809581798439	25.24121939091343	24.16141504551405
19	25.606264949224645	25.2634666065907	25.024228272440453	24.106040171744205
20	25.67953574316913	24.965473993114077	24.95669662472133	24.398293638995465
21	25.951997824033974	24.384368566425067	24.874265395796954	24.789368213744005
22	25.623561942693335	24.32466165222796	24.878347010997096	25.17342939408161
23	25.21628476779862	24.364388397770153	25.048594624681048	25.37073220975018
24	24.911507478226984	24.927440191436116	24.971737193447492	25.189315136889405
25	24.840340850173536	24.708892724910488	24.980379359492833	25.470387065423143
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	452.0
1	452.0
2	463.5
3	475.0
4	475.0
5	475.0
6	589.0
7	703.0
8	703.0
9	703.0
10	999.5
11	1296.0
12	1296.0
13	1296.0
14	1845.0
15	2394.0
16	2394.0
17	2394.0
18	4795.5
19	7197.0
20	7197.0
21	7197.0
22	12542.5
23	17888.0
24	17888.0
25	17888.0
26	28698.0
27	39508.0
28	39508.0
29	39508.0
30	54533.0
31	69558.0
32	69558.0
33	69558.0
34	89559.5
35	109561.0
36	109561.0
37	109561.0
38	134575.5
39	159590.0
40	159590.0
41	159590.0
42	184358.0
43	209126.0
44	209126.0
45	209126.0
46	233268.5
47	257411.0
48	257411.0
49	257411.0
50	270724.5
51	284038.0
52	284038.0
53	284038.0
54	275442.0
55	266846.0
56	266846.0
57	266846.0
58	240979.5
59	215113.0
60	215113.0
61	215113.0
62	186077.0
63	157041.0
64	157041.0
65	157041.0
66	125213.5
67	93386.0
68	93386.0
69	93386.0
70	70114.0
71	46842.0
72	46842.0
73	46842.0
74	33321.0
75	19800.0
76	19800.0
77	19800.0
78	13987.5
79	8175.0
80	8175.0
81	8175.0
82	5676.0
83	3177.0
84	3177.0
85	3177.0
86	2110.5
87	1044.0
88	1044.0
89	1044.0
90	677.5
91	311.0
92	311.0
93	311.0
94	176.0
95	41.0
96	41.0
97	41.0
98	24.5
99	8.0
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.10157378483100546
2	0.0
3	0.04297352435157924
4	0.05550535494761238
5	0.07539392820123583
6	0.008016312688960473
7	8.625146564071394E-4
8	0.007204534188812577
9	5.07361562592435E-5
10	0.0
11	0.007407478813849551
12	0.0
13	0.003551530938147044
14	0.06372461226160983
15	0.022882006472918815
16	0.0033485863131100706
17	1.5220846877773048E-4
18	0.008168521157738202
19	0.00725527034507182
20	1.5220846877773048E-4
21	0.018366488565846145
22	3.0441693755546095E-4
23	0.004261837125776454
24	0.009792078158033995
25	0.1859480126901274
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1970981.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.57184466869968
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.35123044012421	28.474736616454905
2	15.211820213709442	13.256141349598677
3	6.815441697141552	8.90884100929292
4	3.813657519363923	6.646723722133735
5	2.3553158306533164	5.131272775947783
6	1.5316428665547805	4.0041903041668085
7	1.000527088409003	3.05163676180884
8	0.7096648543481671	2.473712544039498
9	0.5385529057424866	2.111916919939964
>10	2.5247045188127384	18.76733895503759
>50	0.10560692142760318	3.133329962757729
>100	0.03914071592200302	2.86619823109409
>500	0.0017572355157466767	0.5177902882450004
>1k	9.371922750648943E-4	0.6561705594824014
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2931	0.14870767399584267	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.5220846877773048E-4
2	0.0	0.0	0.0	0.0	1.5220846877773048E-4
3	0.0	0.0	0.0	0.0	1.5220846877773048E-4
4	0.0	0.0	0.0	0.0	1.5220846877773048E-4
5	0.0	0.0	0.0	0.0	1.5220846877773048E-4
6	0.0	0.0	0.0	0.0	2.0294462503697396E-4
7	0.0	0.0	0.0	0.0	2.0294462503697396E-4
8	0.0	0.0	0.0	0.0	2.5368078129621744E-4
9	0.0	0.0	0.0	0.0	2.5368078129621744E-4
10	0.0	0.0	0.0	0.0	3.0441693755546095E-4
11	0.0	0.0	0.0	0.0	3.0441693755546095E-4
12	0.0	0.0	0.0	0.0	5.073615625924349E-4
13	0.0	0.0	0.0	0.0	5.073615625924349E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACCGAC	40	0.0053068795	14.239002	7
TACACCG	90	5.4097836E-7	12.66621	5
GGTATCA	920	0.0	12.62012	1
TAGCACT	150	1.7462298E-10	11.407409	4
GTATCAA	2145	0.0	11.313693	1
CTAAGAC	120	1.2638884E-7	11.090817	3
TAAGACC	125	2.2207314E-7	10.6469145	4
CTTATAC	225	0.0	10.574237	1
ATAGCAC	270	0.0	10.210594	3
TACCGTG	85	6.641585E-4	10.05106	7
ACAACCG	95	1.6449284E-4	9.999639	5
GTATTAC	105	4.0267154E-5	9.969995	1
CCCTAAT	80	0.004450949	9.516813	1
CCCTAGA	410	0.0	9.516812	1
CCTAGAC	130	4.2026077E-6	9.506415	3
TCTTATA	110	6.7868634E-5	9.506415	2
GTAGGAC	120	1.6870355E-5	9.506415	3
TATTAGG	150	2.6224552E-7	9.506415	2
TTAGGGT	170	1.6470949E-8	9.506174	4
CTACACG	80	0.0044922126	9.506174	4
>>END_MODULE