FastQCFastQC Report
Mon 15 Aug 2016
SRR2921194_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921194_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2635336
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGAC400.005295248214.2439673
GGTATCA14650.011.9990211
TACGTAC600.005885238311.08137312
GTATCAA32600.010.6969551
TGCACCG1252.2544737E-710.6356965
GTCCTAC1106.0345883E-610.3657391
TGTGCGA1106.067483E-610.36101710
GTCTTAG1752.3464963E-1010.3163791
TGCCGGT3550.010.21198919
CCGATCA750.002656682610.129629
TAACCGT750.00265710110.1294276
ATGCGAG856.6069164E-410.05704511
CCGGGTA800.00436273789.54014919
CCGGTTA900.00111585799.4983214
CCCGTCC1002.767165E-49.4963388
GACCCGT900.00111799059.4963376
TCAACGG1201.7094288E-59.4961584
TTAGAAC1401.0653312E-69.4959773
GTTCTAA2750.09.32916451
ATTAGAC1651.0732765E-79.20822053
Produced by FastQC (version 0.11.5)