##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921194_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2635336
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.67018588900998	31.0	28.0	31.0	16.0	34.0
2	27.24880622432965	31.0	27.0	31.0	10.0	34.0
3	27.125471666611013	31.0	27.0	31.0	10.0	34.0
4	31.03154853878215	35.0	32.0	37.0	16.0	37.0
5	30.700905311504872	35.0	31.0	37.0	10.0	37.0
6	31.00672475919579	35.0	32.0	37.0	15.0	37.0
7	31.066196872049712	35.0	32.0	37.0	11.0	37.0
8	30.745936381546795	35.0	31.0	37.0	10.0	37.0
9	32.379986840387716	37.0	32.0	39.0	11.0	39.0
10	31.733934116939928	37.0	31.0	39.0	10.0	39.0
11	32.1861990273726	37.0	32.0	39.0	10.0	39.0
12	31.890996062741145	37.0	32.0	39.0	10.0	39.0
13	31.898509715649162	37.0	32.0	39.0	8.0	39.0
14	33.02788866391231	38.0	32.0	40.0	8.0	41.0
15	32.666740028595974	38.0	32.0	40.0	8.0	41.0
16	32.59165017288118	38.0	32.0	40.0	2.0	41.0
17	32.918080654611025	38.0	32.0	40.0	2.0	41.0
18	32.966860772212726	38.0	32.0	40.0	2.0	41.0
19	32.670194237091586	38.0	32.0	40.0	2.0	41.0
20	32.54514832264273	38.0	32.0	40.0	2.0	41.0
21	32.501453704575056	38.0	32.0	40.0	2.0	41.0
22	31.724513306842088	38.0	30.0	40.0	2.0	41.0
23	31.82863854931591	38.0	31.0	40.0	2.0	41.0
24	31.458606416790875	38.0	30.0	40.0	2.0	41.0
25	30.951533694375215	38.0	30.0	40.0	2.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	183884.0
3	13.0
4	1421.0
5	8731.0
6	10474.0
7	9232.0
8	8008.0
9	7452.0
10	7011.0
11	6913.0
12	6591.0
13	6381.0
14	6372.0
15	6743.0
16	7210.0
17	7899.0
18	9108.0
19	11253.0
20	14216.0
21	17795.0
22	22168.0
23	26171.0
24	30484.0
25	34622.0
26	39130.0
27	44319.0
28	49471.0
29	59006.0
30	69505.0
31	82707.0
32	104397.0
33	132386.0
34	169719.0
35	219730.0
36	288528.0
37	380537.0
38	545749.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.94982698699252	17.384732645937497	12.005880468433288	25.659559898636697
2	16.144190153812044	19.615306640789615	38.56113567456874	25.679367530829605
3	19.046207620243987	22.853559168942166	29.858764191418828	28.241469019395016
4	14.018385153133078	15.401199440923758	34.376357890766094	36.20405751517707
5	14.303382427165676	36.34790462564846	33.87853925292874	15.470173694257122
6	34.65975086887014	33.44718419288139	16.78248439752455	15.11058054072391
7	30.028350434287976	31.174131471486582	19.912774316350774	18.88474377787467
8	26.790599896026624	34.19536369811292	20.027397364236954	18.9866390416235
9	27.120835401496972	15.792535485134268	18.877969996827552	38.20865911654121
10	16.14916260157974	27.654316771305893	32.723636701710845	23.472883925403522
11	35.97354012244012	22.36979921702686	21.85998624711774	19.796674413415282
12	23.771681592575995	24.468118614396953	28.859975249769747	22.90022454325731
13	28.68160867285021	21.172821750128833	24.930497737144453	25.2150718398765
14	23.24207475843151	20.64614091941189	25.315345505275776	30.796438816880816
15	25.018300492377165	27.51646475091787	22.72919255836899	24.736042198335976
16	24.60810924167207	26.839909982505876	24.433965092979996	24.118015682842056
17	23.651391483847064	26.93682996619321	25.308174744589053	24.10360380537067
18	23.6280544504589	26.761126811453938	26.057078400250195	23.553740337836963
19	24.77318010861851	26.002292298408676	26.210686513011826	23.013841079960986
20	25.112071203355534	25.383767019414545	25.876834772206646	23.62732700502328
21	25.432704624511103	25.144405668057683	25.52056824802212	23.902321459409094
22	24.53959886187093	24.59929068056263	26.712790898003263	24.148319559563173
23	24.857454492460572	25.068426397788496	25.603161808126764	24.470957301624168
24	24.31242953140352	25.447782746289523	25.753508771596927	24.486278950710027
25	23.969414551455227	25.19651482328437	26.47785894236315	24.356211682897253
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1354.0
1	1354.0
2	1343.5
3	1333.0
4	1333.0
5	1333.0
6	1601.0
7	1869.0
8	1869.0
9	1869.0
10	2430.0
11	2991.0
12	2991.0
13	2991.0
14	4411.0
15	5831.0
16	5831.0
17	5831.0
18	10471.5
19	15112.0
20	15112.0
21	15112.0
22	25022.0
23	34932.0
24	34932.0
25	34932.0
26	53258.0
27	71584.0
28	71584.0
29	71584.0
30	96328.0
31	121072.0
32	121072.0
33	121072.0
34	148560.5
35	176049.0
36	176049.0
37	176049.0
38	209352.5
39	242656.0
40	242656.0
41	242656.0
42	273402.5
43	304149.0
44	304149.0
45	304149.0
46	329836.5
47	355524.0
48	355524.0
49	355524.0
50	361527.0
51	367530.0
52	367530.0
53	367530.0
54	347890.0
55	328250.0
56	328250.0
57	328250.0
58	291632.5
59	255015.0
60	255015.0
61	255015.0
62	215488.0
63	175961.0
64	175961.0
65	175961.0
66	137644.5
67	99328.0
68	99328.0
69	99328.0
70	72703.0
71	46078.0
72	46078.0
73	46078.0
74	32224.0
75	18370.0
76	18370.0
77	18370.0
78	12659.5
79	6949.0
80	6949.0
81	6949.0
82	4669.5
83	2390.0
84	2390.0
85	2390.0
86	1590.5
87	791.0
88	791.0
89	791.0
90	488.5
91	186.0
92	186.0
93	186.0
94	104.5
95	23.0
96	23.0
97	23.0
98	16.0
99	9.0
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03232984332927566
2	0.05092329782615955
3	0.014457359516964818
4	0.010435102013557285
5	0.009334673073945789
6	6.450790335653594E-4
7	0.003908420026896001
8	0.001745507973176855
9	0.005615982174569011
10	0.003453070120849865
11	0.009562348026968858
12	0.007930677530303536
13	0.006450790335653593
14	0.014002009610918683
15	0.006109277906118993
16	0.027966073396333526
17	0.03620031753066782
18	0.02178090383920684
19	0.01616492166463783
20	0.015330113503553249
21	0.02166706636269531
22	0.005084740617515186
23	0.014722980295491732
24	0.011269910174641868
25	0.45523606856962456
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2635336.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.07723799307551
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.20892644944378	31.889561752562795
2	12.885852325165583	11.8748896866056
3	6.037472657959323	8.345701936124335
4	3.394107153741124	6.255643323877195
5	2.180652972741643	5.0239233002662145
6	1.4904429541717767	4.12052968286853
7	1.023489283155736	3.3011691498330364
8	0.72766899281297	2.6823181889619816
9	0.5361570869311912	2.2234173926582317
>10	2.399763630923486	18.12694999289811
>50	0.08342575893264263	2.595338437204909
>100	0.029304428718634472	2.4146349170691335
>500	0.001881209895222068	0.5869876742437424
>1k	8.550954069132923E-4	0.5589345648260432
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	1.517833020153787E-4
7	0.0	0.0	0.0	0.0	1.517833020153787E-4
8	0.0	0.0	0.0	0.0	1.517833020153787E-4
9	0.0	0.0	0.0	0.0	1.8972912751922334E-4
10	0.0	0.0	0.0	0.0	2.2767495302306802E-4
11	0.0	0.0	0.0	0.0	2.2767495302306802E-4
12	0.0	0.0	0.0	0.0	2.2767495302306802E-4
13	0.0	0.0	0.0	0.0	2.2767495302306802E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTACGAC	40	0.0052952482	14.243967	3
GGTATCA	1465	0.0	11.999021	1
TACGTAC	60	0.0058852383	11.081373	12
GTATCAA	3260	0.0	10.696955	1
TGCACCG	125	2.2544737E-7	10.635696	5
GTCCTAC	110	6.0345883E-6	10.365739	1
TGTGCGA	110	6.067483E-6	10.361017	10
GTCTTAG	175	2.3464963E-10	10.316379	1
TGCCGGT	355	0.0	10.211989	19
CCGATCA	75	0.0026566826	10.12962	9
TAACCGT	75	0.002657101	10.129427	6
ATGCGAG	85	6.6069164E-4	10.057045	11
CCGGGTA	80	0.0043627378	9.540149	19
CCGGTTA	90	0.0011158579	9.49832	14
CCCGTCC	100	2.767165E-4	9.496338	8
GACCCGT	90	0.0011179905	9.496337	6
TCAACGG	120	1.7094288E-5	9.496158	4
TTAGAAC	140	1.0653312E-6	9.495977	3
GTTCTAA	275	0.0	9.3291645	1
ATTAGAC	165	1.0732765E-7	9.2082205	3
>>END_MODULE