FastQCFastQC Report
Mon 15 Aug 2016
SRR2921194_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921194_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2635336
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT53530.20312400392208052No Hit
GGTATCAACGCAGAGTACTTTTTTT35060.1330380642164794No Hit
TATCAACGCAGAGTACTTTTTTTTT34420.13060953138423337No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16500.013.26201251
ATCGACC701.09304514E-412.2123818
TTACGTA550.003052094312.100054
TGTTCGA907.469449E-611.61062310
AACCGTA600.005910978711.0748777
TACACCG1408.358256E-910.8573065
CTAGGGT2850.010.6747324
GACCCGT1002.4204088E-510.4420286
GTATCAA34600.010.2289821
CCTACAC2700.010.2114193
AAACCGT750.002665437310.1256036
GTCTTAT1606.02995E-910.1086811
GCCTACG2201.8189894E-129.9465091
TAGGACA2600.09.8728444
TCCGTAG900.00109909379.5140521
GCTATTC1201.6861104E-59.5071833
GCGGTTG1002.7576316E-49.49960113
ACCGTCC1502.655961E-79.498528
GAACCGT1304.2823067E-69.4927526
TCTAGGG3750.09.380423
Produced by FastQC (version 0.11.5)