##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921194_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2635336
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.060209020785205	31.0	30.0	33.0	26.0	34.0
2	30.297280119119534	31.0	30.0	34.0	27.0	34.0
3	30.414649213610712	31.0	30.0	34.0	27.0	34.0
4	34.13678028152767	37.0	35.0	37.0	30.0	37.0
5	33.7729287650607	37.0	35.0	37.0	28.0	37.0
6	33.77615416022852	37.0	35.0	37.0	29.0	37.0
7	33.652848820795526	37.0	35.0	37.0	28.0	37.0
8	33.61509500116873	37.0	35.0	37.0	28.0	37.0
9	35.05022737138642	39.0	35.0	39.0	28.0	39.0
10	34.92261973425779	39.0	35.0	39.0	28.0	39.0
11	34.97431447071645	39.0	35.0	39.0	28.0	39.0
12	34.845941466287414	39.0	35.0	39.0	27.0	39.0
13	34.836148028183125	39.0	35.0	39.0	27.0	39.0
14	35.83954569739874	39.0	36.0	41.0	27.0	41.0
15	35.846623352771715	39.0	36.0	41.0	27.0	41.0
16	35.82465196088848	39.0	36.0	41.0	27.0	41.0
17	35.810920884471656	39.0	36.0	41.0	27.0	41.0
18	35.768659100775004	39.0	36.0	41.0	27.0	41.0
19	35.80867411214358	40.0	36.0	41.0	27.0	41.0
20	35.74536909145551	40.0	36.0	41.0	27.0	41.0
21	35.68630413730924	39.0	36.0	41.0	27.0	41.0
22	35.605556179553574	39.0	36.0	41.0	27.0	41.0
23	35.48757198323098	39.0	36.0	41.0	27.0	41.0
24	35.4236442715464	39.0	36.0	41.0	27.0	41.0
25	35.29444366866312	39.0	35.0	41.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	59842.0
3	14.0
4	896.0
5	7933.0
6	9185.0
7	7121.0
8	5575.0
9	4876.0
10	4362.0
11	4104.0
12	3833.0
13	3827.0
14	3768.0
15	3744.0
16	3840.0
17	4082.0
18	4673.0
19	5459.0
20	6746.0
21	8935.0
22	11325.0
23	14328.0
24	16889.0
25	20115.0
26	23240.0
27	26525.0
28	30679.0
29	37865.0
30	49261.0
31	63659.0
32	82548.0
33	108193.0
34	143875.0
35	193112.0
36	264483.0
37	402846.0
38	993578.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.854659300463965	18.684566868420603	9.47349629379222	30.98727753732321
2	15.228646366155965	20.64241523661499	35.10224123223756	29.026697164991482
3	18.586875274986628	23.204107175599294	27.792374134420868	30.41664341499321
4	13.834122198932327	16.044679646660093	32.772705338861655	37.348492815545924
5	15.020079528298162	37.170707886910634	32.38298586534869	15.426226719442512
6	34.95574801470374	34.34032229971437	16.023609154201367	14.680320531380525
7	30.427562989241196	31.577171899848633	18.96930594297263	19.025959167937536
8	28.061705987733333	34.613290246050596	18.245448001363048	19.079555764853026
9	27.27197870479465	15.598434354645615	18.586289788584754	38.54329715197499
10	17.02025851731999	28.260229435639328	30.950702301338424	23.768809745702253
11	36.46207701283547	22.160799157583174	21.503296619897355	19.873827209684
12	24.185189288955943	24.546129981148514	28.242546680954533	23.026134048941007
13	29.069156333635508	20.80810710202446	24.843064539814723	25.2796720245253
14	23.35481367609243	20.47450131573902	25.16810744297104	31.002577565197516
15	25.165212031595612	27.506342046974414	22.34806793391215	24.98037798751782
16	25.082779258094455	26.776803745424658	23.772730618876935	24.36768637760395
17	23.647675644785686	26.848978857700335	25.303405679661733	24.199939817852243
18	23.66360247033042	26.496323913064103	26.221362096934854	23.618711519670622
19	24.99133865827184	25.815351957842697	25.890600328694596	23.302709055190864
20	25.123694045496336	25.278702919137736	25.8400741006924	23.75752893467353
21	25.316163632065035	24.936254960424755	25.719790505660683	24.027790901849528
22	25.290314354960074	24.680068894519213	25.363398098077166	24.666218652443543
23	24.726939767375143	24.89894949979433	25.650168708420544	24.723942024409975
24	24.404915499670622	25.418972577996218	25.536190791331087	24.639921131002072
25	24.36794795187464	25.18986648907783	25.745629839789935	24.696555719257592
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	691.0
1	691.0
2	711.0
3	731.0
4	731.0
5	731.0
6	1089.5
7	1448.0
8	1448.0
9	1448.0
10	1838.0
11	2228.0
12	2228.0
13	2228.0
14	3092.0
15	3956.0
16	3956.0
17	3956.0
18	8428.0
19	12900.0
20	12900.0
21	12900.0
22	22072.0
23	31244.0
24	31244.0
25	31244.0
26	49257.5
27	67271.0
28	67271.0
29	67271.0
30	89565.0
31	111859.0
32	111859.0
33	111859.0
34	138284.5
35	164710.0
36	164710.0
37	164710.0
38	196790.0
39	228870.0
40	228870.0
41	228870.0
42	260234.0
43	291598.0
44	291598.0
45	291598.0
46	320946.5
47	350295.0
48	350295.0
49	350295.0
50	362878.0
51	375461.0
52	375461.0
53	375461.0
54	358771.0
55	342081.0
56	342081.0
57	342081.0
58	305475.0
59	268869.0
60	268869.0
61	268869.0
62	229448.5
63	190028.0
64	190028.0
65	190028.0
66	149724.5
67	109421.0
68	109421.0
69	109421.0
70	79585.5
71	49750.0
72	49750.0
73	49750.0
74	34876.5
75	20003.0
76	20003.0
77	20003.0
78	13909.0
79	7815.0
80	7815.0
81	7815.0
82	5285.0
83	2755.0
84	2755.0
85	2755.0
86	1896.0
87	1037.0
88	1037.0
89	1037.0
90	645.0
91	253.0
92	253.0
93	253.0
94	154.0
95	55.0
96	55.0
97	55.0
98	31.0
99	7.0
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.09831763388046154
2	0.0
3	0.0423854870877945
4	0.052554968322824865
5	0.06860605251095117
6	0.003301286818834487
7	1.8972912751922334E-4
8	0.002466478657749904
9	3.7945825503844674E-5
10	0.0
11	0.002314695355734525
12	0.0
13	6.450790335653594E-4
14	0.055211176108094
15	0.020490745772076123
16	5.312415570538254E-4
17	1.1383747651153401E-4
18	0.002314695355734525
19	0.0023905870067422144
20	1.1383747651153401E-4
21	0.00868959404038043
22	1.1383747651153401E-4
23	7.589165100768934E-4
24	0.0030356660403075735
25	0.1722740477874548
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2635336.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.7480065956253
#Duplication Level	Percentage of deduplicated	Percentage of total
1	57.715570200510705	19.4778544379711
2	15.635209138464804	10.553142822577824
3	7.88651002232054	7.984619767492158
4	4.852082828017277	6.549924931297893
5	3.199444697505618	5.398744037687899
6	2.221234699560287	4.497734597471541
7	1.6123364055898766	3.8089197755149766
8	1.232967285980722	3.3288150479574106
9	0.9188976489660022	2.7909867526208303
>10	4.474744224486803	25.375088683503584
>50	0.1789458620357766	4.084530558133407
>100	0.06662768670920655	4.0087333733951365
>500	0.0033933124078182105	0.8133034946513131
>1k	0.0019228770310952244	1.1232642745514598
>5k	1.1311041359383674E-4	0.20433744517345084
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	5353	0.20312400392208052	No Hit
GGTATCAACGCAGAGTACTTTTTTT	3506	0.1330380642164794	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3442	0.13060953138423337	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.7945825503844674E-5
2	0.0	0.0	0.0	0.0	3.7945825503844674E-5
3	0.0	0.0	0.0	0.0	3.7945825503844674E-5
4	0.0	0.0	0.0	0.0	7.589165100768935E-5
5	0.0	0.0	0.0	0.0	7.589165100768935E-5
6	0.0	0.0	0.0	0.0	1.517833020153787E-4
7	0.0	0.0	0.0	0.0	1.517833020153787E-4
8	0.0	0.0	0.0	0.0	1.517833020153787E-4
9	0.0	0.0	0.0	3.7945825503844674E-5	1.8972912751922334E-4
10	0.0	0.0	0.0	3.7945825503844674E-5	1.8972912751922334E-4
11	0.0	0.0	0.0	3.7945825503844674E-5	1.8972912751922334E-4
12	0.0	0.0	0.0	3.7945825503844674E-5	1.8972912751922334E-4
13	0.0	0.0	0.0	3.7945825503844674E-5	1.8972912751922334E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1650	0.0	13.2620125	1
ATCGACC	70	1.09304514E-4	12.212381	8
TTACGTA	55	0.0030520943	12.10005	4
TGTTCGA	90	7.469449E-6	11.610623	10
AACCGTA	60	0.0059109787	11.074877	7
TACACCG	140	8.358256E-9	10.857306	5
CTAGGGT	285	0.0	10.674732	4
GACCCGT	100	2.4204088E-5	10.442028	6
GTATCAA	3460	0.0	10.228982	1
CCTACAC	270	0.0	10.211419	3
AAACCGT	75	0.0026654373	10.125603	6
GTCTTAT	160	6.02995E-9	10.108681	1
GCCTACG	220	1.8189894E-12	9.946509	1
TAGGACA	260	0.0	9.872844	4
TCCGTAG	90	0.0010990937	9.514052	1
GCTATTC	120	1.6861104E-5	9.507183	3
GCGGTTG	100	2.7576316E-4	9.499601	13
ACCGTCC	150	2.655961E-7	9.49852	8
GAACCGT	130	4.2823067E-6	9.492752	6
TCTAGGG	375	0.0	9.38042	3
>>END_MODULE