FastQCFastQC Report
Mon 15 Aug 2016
SRR2921193_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921193_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2841661
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGACCG550.00307822312.086349
GGTATCA16200.011.9649711
GTATTAC1850.011.2991921
AACTTAT1252.2550194E-710.6356047
TAATACC1659.349606E-1010.3606324
GTATCAA31750.010.2048621
CCCGTAG1451.6145532E-79.8292341
TTAATAC1651.027729E-89.7852133
GTATAGG2151.0913936E-119.7225611
GTACTAA1304.2322845E-69.5015941
TAGACTG2950.09.336115
GTTCTAG1951.6789272E-99.2579631
TGGACCG1856.7393557E-99.24045
TCGCGCA1151.1157333E-49.0835229
GTTCGCG1151.11616115E-49.0832027
GTCTTAA2553.6379788E-128.9426771
ACCGACC850.0074610158.9377988
CTAAACT3200.08.9036674
GGCTAAT1401.0833815E-58.8229081
CGTAGGG1401.0894231E-58.8190263
Produced by FastQC (version 0.11.5)