##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921193_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2841661
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.314663149474903	31.0	28.0	31.0	15.0	34.0
2	27.074701732543044	31.0	27.0	31.0	10.0	34.0
3	26.83501304342777	31.0	26.0	31.0	10.0	34.0
4	30.728997934658636	35.0	31.0	37.0	16.0	37.0
5	30.491872183205526	35.0	30.0	37.0	10.0	37.0
6	31.078372473000826	35.0	32.0	37.0	15.0	37.0
7	31.080556406974654	35.0	32.0	37.0	11.0	37.0
8	30.714724240505817	35.0	31.0	37.0	10.0	37.0
9	32.39805240667342	37.0	32.0	39.0	10.0	39.0
10	31.741687696034116	37.0	31.0	39.0	10.0	39.0
11	32.210048278102136	37.0	32.0	39.0	10.0	39.0
12	31.901860918666934	37.0	32.0	39.0	8.0	39.0
13	31.913766983464953	37.0	32.0	39.0	8.0	39.0
14	33.030192200969786	38.0	32.0	40.0	8.0	41.0
15	32.681326871854175	38.0	32.0	40.0	7.0	41.0
16	32.6044482434745	38.0	32.0	40.0	2.0	41.0
17	32.921695445023175	38.0	32.0	40.0	2.0	41.0
18	32.976324762172545	38.0	32.0	40.0	2.0	41.0
19	32.66814725612942	38.0	32.0	40.0	2.0	41.0
20	32.55401506372505	38.0	32.0	40.0	2.0	41.0
21	32.4991165378277	38.0	32.0	40.0	2.0	41.0
22	31.724170476351684	38.0	30.0	40.0	2.0	41.0
23	31.834469347328902	38.0	31.0	40.0	2.0	41.0
24	31.465559755368428	38.0	30.0	40.0	2.0	41.0
25	30.96115053836471	38.0	30.0	40.0	2.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	203463.0
3	14.0
4	1551.0
5	8688.0
6	10795.0
7	9748.0
8	8557.0
9	7909.0
10	7592.0
11	7417.0
12	7271.0
13	7019.0
14	7029.0
15	7138.0
16	7735.0
17	8688.0
18	9825.0
19	12265.0
20	15306.0
21	19346.0
22	23637.0
23	28294.0
24	32265.0
25	37061.0
26	41687.0
27	47159.0
28	52750.0
29	62444.0
30	74368.0
31	88595.0
32	111441.0
33	141044.0
34	181926.0
35	237627.0
36	316723.0
37	426934.0
38	570350.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.18502221252209	17.17999535332343	11.956743665382964	25.678238768771518
2	16.33889609104717	19.2110550039117	38.827821158947366	25.622227746093756
3	19.653870290052218	22.75553656545876	29.131856540455654	28.45873660403337
4	13.980555188949012	15.47930139457129	33.970876776208705	36.569266640270996
5	14.670989861748557	36.141544870041955	33.839207691092156	15.348257577117334
6	35.02446824758362	33.22191887648057	16.817213428010987	14.936399447924826
7	30.303808792151628	30.791252823716015	19.865869310381598	19.039069073750763
8	26.98287126367936	33.77344938536366	20.062331103016295	19.181348247940683
9	27.360143982420293	15.498240691926469	18.51757090901924	38.62404441663399
10	16.153338576941145	27.637649104099694	32.48678980347029	23.722222515488877
11	36.33163775812414	22.14627934862601	21.4368196156923	20.085263277557555
12	23.91317354310845	24.00168649732244	28.860607758450783	23.224532201118326
13	29.20127217588602	20.68862485135131	24.890979228423987	25.219123744338678
14	23.251285101620258	20.0928476021702	25.16212254264461	31.493744753564933
15	24.872635898446575	27.453914536893002	22.678235836506584	24.995213728153836
16	24.864262440966833	26.629660346327906	24.26991779710896	24.2361594155963
17	23.912656991225216	26.69189674443985	25.310567588737996	24.08487867559694
18	24.17186910413886	26.460624799364723	25.722181391183874	23.64532470531254
19	25.04811421829706	25.78351910278774	25.93352846517403	23.234838213741167
20	25.397689676047815	25.261759281420265	25.598100775739486	23.742450266792435
21	25.852431024268885	25.05752450841493	25.1317950990562	23.958249368259988
22	24.717630936401765	24.38611519151545	26.504965345694902	24.391288526387882
23	25.071501629244608	24.780637999376317	25.584349396767696	24.563510974611376
24	24.805336407969275	25.09509691297541	25.59268394319244	24.50688273586287
25	24.287029462071658	24.879530552748825	26.335310576068455	24.498129409111062
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1151.0
1	1151.0
2	1190.5
3	1230.0
4	1230.0
5	1230.0
6	1561.0
7	1892.0
8	1892.0
9	1892.0
10	2394.0
11	2896.0
12	2896.0
13	2896.0
14	4279.5
15	5663.0
16	5663.0
17	5663.0
18	10119.0
19	14575.0
20	14575.0
21	14575.0
22	23721.5
23	32868.0
24	32868.0
25	32868.0
26	50622.0
27	68376.0
28	68376.0
29	68376.0
30	92974.5
31	117573.0
32	117573.0
33	117573.0
34	148549.5
35	179526.0
36	179526.0
37	179526.0
38	216986.0
39	254446.0
40	254446.0
41	254446.0
42	291373.5
43	328301.0
44	328301.0
45	328301.0
46	359516.0
47	390731.0
48	390731.0
49	390731.0
50	400606.0
51	410481.0
52	410481.0
53	410481.0
54	387860.5
55	365240.0
56	365240.0
57	365240.0
58	323824.5
59	282409.0
60	282409.0
61	282409.0
62	236708.0
63	191007.0
64	191007.0
65	191007.0
66	149478.5
67	107950.0
68	107950.0
69	107950.0
70	79958.5
71	51967.0
72	51967.0
73	51967.0
74	36569.0
75	21171.0
76	21171.0
77	21171.0
78	14698.0
79	8225.0
80	8225.0
81	8225.0
82	5531.0
83	2837.0
84	2837.0
85	2837.0
86	1863.0
87	889.0
88	889.0
89	889.0
90	555.5
91	222.0
92	222.0
93	222.0
94	124.0
95	26.0
96	26.0
97	26.0
98	17.5
99	9.0
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03241062181590274
2	0.05148397363373042
3	0.014568944008451394
4	0.010064536199075118
5	0.008586527386623528
6	6.68623034204291E-4
7	0.003659831345118225
8	0.002181822532666634
9	0.007143709260182689
10	0.003730212717139729
11	0.012105595987698744
12	0.009466294536892331
13	0.008093857782472997
14	0.015695045960795462
15	0.007073327888161184
16	0.031143757119515662
17	0.03997661930821446
18	0.02410561991736523
19	0.01777129643542984
20	0.017630533691386834
21	0.024563098835505007
22	0.005876844563795611
23	0.01643405036702126
24	0.012281549417752504
25	0.46251118623931564
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2841661.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.248890099828486
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.60379345931781	31.042455106720045
2	12.884325846758705	11.660028885007284
3	6.224453766802834	8.449488732765747
4	3.5315379785595837	6.391926955008523
5	2.211739642793824	5.003938201310583
6	1.5017218934623309	4.077074935067001
7	1.0243348911734214	3.244501184128814
8	0.760372735797752	2.7524817885614725
9	0.5788355993505391	2.357250157879281
>10	2.5559565758084553	19.285456987583295
>50	0.09183026387864247	2.8044266734754837
>100	0.02964527716488192	2.20278183668829
>500	8.067050729459297E-4	0.2784411400912843
>1k	6.453640583552043E-4	0.4497474157129553
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	3.5190686010752165E-5	0.0	0.0	0.0
8	0.0	3.5190686010752165E-5	0.0	0.0	0.0
9	0.0	3.5190686010752165E-5	0.0	0.0	0.0
10	0.0	3.5190686010752165E-5	0.0	0.0	0.0
11	0.0	3.5190686010752165E-5	0.0	3.5190686010752165E-5	0.0
12	0.0	3.5190686010752165E-5	0.0	7.038137202150433E-5	0.0
13	3.5190686010752165E-5	3.5190686010752165E-5	0.0	7.038137202150433E-5	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGACCG	55	0.003078223	12.08634	9
GGTATCA	1620	0.0	11.964971	1
GTATTAC	185	0.0	11.299192	1
AACTTAT	125	2.2550194E-7	10.635604	7
TAATACC	165	9.349606E-10	10.360632	4
GTATCAA	3175	0.0	10.204862	1
CCCGTAG	145	1.6145532E-7	9.829234	1
TTAATAC	165	1.027729E-8	9.785213	3
GTATAGG	215	1.0913936E-11	9.722561	1
GTACTAA	130	4.2322845E-6	9.501594	1
TAGACTG	295	0.0	9.33611	5
GTTCTAG	195	1.6789272E-9	9.257963	1
TGGACCG	185	6.7393557E-9	9.2404	5
TCGCGCA	115	1.1157333E-4	9.083522	9
GTTCGCG	115	1.11616115E-4	9.083202	7
GTCTTAA	255	3.6379788E-12	8.942677	1
ACCGACC	85	0.007461015	8.937798	8
CTAAACT	320	0.0	8.903667	4
GGCTAAT	140	1.0833815E-5	8.822908	1
CGTAGGG	140	1.0894231E-5	8.819026	3
>>END_MODULE