FastQCFastQC Report
Mon 15 Aug 2016
SRR2921193_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921193_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2841661
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT49240.17327893791694365No Hit
TATCAACGCAGAGTACTTTTTTTTT31240.10993570309758977No Hit
GGTATCAACGCAGAGTACTTTTTTT30950.10891517320327794No Hit
GGTATCAACGCAGAGTACATGGGGG28630.10075093404878345No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGTAC456.737201E-414.7857393
GGATCGT602.5874646E-514.2396146
GGTATCA17850.012.9008091
GAATGCG550.00308632712.0820977
CGTAAGA657.9908234E-411.69860652
TAGGGTG2650.011.1133215
ACGATTC600.005888034611.0807148
TTGCGCG600.005908122811.075644518
CTTATAC2200.010.8132721
GTATCAA36900.010.6245531
TTCGTTG1451.425542E-810.49191919
CGTCGAC856.543771E-410.06761319
CGTAGGG1252.554154E-69.8853233
TTACCCT1304.2123465E-69.5051184
ACAACCG1304.240781E-69.5000965
GTGCTAT2850.09.3487381
TATACAC4000.09.267493
TTAGCCT1856.6411303E-99.2482224
TCTATAC1856.6411303E-99.2482223
TATACTG2900.09.1725075
Produced by FastQC (version 0.11.5)