##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921193_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2841661
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.69087727213063	31.0	30.0	33.0	26.0	34.0
2	29.948702888908986	31.0	30.0	34.0	26.0	34.0
3	30.077215403244793	31.0	30.0	34.0	26.0	34.0
4	33.78461329483003	35.0	35.0	37.0	30.0	37.0
5	33.36502418831803	35.0	35.0	37.0	28.0	37.0
6	33.344648781117805	36.0	35.0	37.0	28.0	37.0
7	33.195426548064674	36.0	35.0	37.0	27.0	37.0
8	33.14556240170801	37.0	35.0	37.0	26.0	37.0
9	34.53364915801005	38.0	35.0	39.0	27.0	39.0
10	34.40526896065364	38.0	35.0	39.0	27.0	39.0
11	34.445435257759456	38.0	35.0	39.0	27.0	39.0
12	34.303027349145445	38.0	35.0	39.0	26.0	39.0
13	34.28572303311338	38.0	35.0	39.0	26.0	39.0
14	35.248881552021864	39.0	35.0	41.0	26.0	41.0
15	35.24297866635042	39.0	35.0	41.0	26.0	41.0
16	35.2328968867152	39.0	35.0	41.0	26.0	41.0
17	35.201039462483386	39.0	35.0	41.0	25.0	41.0
18	35.148305163775696	39.0	35.0	41.0	25.0	41.0
19	35.18438019172589	39.0	35.0	41.0	25.0	41.0
20	35.11889489984907	39.0	35.0	41.0	25.0	41.0
21	35.05290039874566	39.0	35.0	41.0	25.0	41.0
22	34.960057163750356	39.0	35.0	41.0	25.0	41.0
23	34.834152279247945	39.0	35.0	41.0	24.0	41.0
24	34.752160796097776	39.0	35.0	41.0	23.0	41.0
25	34.61787841688365	39.0	34.0	41.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	81962.0
3	6.0
4	1354.0
5	11038.0
6	12615.0
7	9703.0
8	7601.0
9	6512.0
10	5856.0
11	5512.0
12	5158.0
13	4826.0
14	4879.0
15	4754.0
16	4852.0
17	5250.0
18	5733.0
19	6797.0
20	8380.0
21	11157.0
22	14136.0
23	17241.0
24	20599.0
25	23986.0
26	27560.0
27	31224.0
28	35807.0
29	44203.0
30	55933.0
31	71711.0
32	92278.0
33	118540.0
34	155883.0
35	206601.0
36	280805.0
37	426705.0
38	1014504.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.728047575065226	18.172303911726022	9.247505458296082	31.85214305491268
2	15.079349718351345	20.61720240380538	34.89360623944939	29.409841638393885
3	18.612174287742068	23.082761611860263	27.42876012482116	30.87630397557651
4	13.889386723942149	15.84437030712217	32.26583805005149	38.000404918884186
5	15.222853770545372	36.910259182079585	32.2344462934702	15.632440753904845
6	35.31932893468575	34.02447397286981	15.852023010728697	14.804174081715745
7	30.75633741604804	31.08047950930004	19.058365635518737	19.104817439133182
8	28.114816634314504	34.72824182057992	18.1464673266602	19.010474218445378
9	27.399673007800374	15.433841792362065	18.15785010018799	39.00863509964957
10	17.07740648866983	28.266637012648587	30.610090366162606	24.045866132518974
11	36.78137337669307	22.06184217425562	20.98796017669997	20.168824272351337
12	24.12937363042249	24.135426428416338	28.272478666526375	23.4627212746348
13	29.387452030594847	20.468952071943992	24.73628923989717	25.40730665756399
14	23.35707289387684	19.87246969121906	24.956430137177293	31.814027277726804
15	25.009547536524384	27.55698867354183	22.17365640202362	25.25980738791016
16	25.35920088821479	26.664179852937814	23.50943376307117	24.467185495776228
17	24.025375326657887	26.584761431530467	25.23178369810864	24.158079543703007
18	24.25809907120961	26.34610911325204	25.658692317464514	23.73709949807383
19	25.328224567962582	25.574067479057955	25.55179170426217	23.545916248717294
20	25.458306383104514	25.136100121830285	25.41340302034939	23.992190474715816
21	25.63157800274707	24.90726087276308	25.269173095324316	24.191988029165536
22	25.507565133698495	24.543479064136918	25.058179124489072	24.89077667767551
23	25.03182124501391	24.748993104370516	25.410368253711308	24.808817396904267
24	24.96537225529375	25.03863949561734	25.283250728801416	24.712737520287494
25	24.75060228011709	24.962858658470605	25.443308125979204	24.843230935433105
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	752.0
1	752.0
2	790.5
3	829.0
4	829.0
5	829.0
6	1154.5
7	1480.0
8	1480.0
9	1480.0
10	1942.0
11	2404.0
12	2404.0
13	2404.0
14	3169.5
15	3935.0
16	3935.0
17	3935.0
18	7882.0
19	11829.0
20	11829.0
21	11829.0
22	20379.5
23	28930.0
24	28930.0
25	28930.0
26	45304.0
27	61678.0
28	61678.0
29	61678.0
30	84933.5
31	108189.0
32	108189.0
33	108189.0
34	135559.0
35	162929.0
36	162929.0
37	162929.0
38	201294.5
39	239660.0
40	239660.0
41	239660.0
42	276658.5
43	313657.0
44	313657.0
45	313657.0
46	348719.0
47	383781.0
48	383781.0
49	383781.0
50	401356.0
51	418931.0
52	418931.0
53	418931.0
54	400102.0
55	381273.0
56	381273.0
57	381273.0
58	341036.0
59	300799.0
60	300799.0
61	300799.0
62	254545.0
63	208291.0
64	208291.0
65	208291.0
66	163129.0
67	117967.0
68	117967.0
69	117967.0
70	87399.0
71	56831.0
72	56831.0
73	56831.0
74	40273.0
75	23715.0
76	23715.0
77	23715.0
78	16484.5
79	9254.0
80	9254.0
81	9254.0
82	6177.0
83	3100.0
84	3100.0
85	3100.0
86	2081.5
87	1063.0
88	1063.0
89	1063.0
90	685.0
91	307.0
92	307.0
93	307.0
94	185.0
95	63.0
96	63.0
97	63.0
98	38.5
99	14.0
100	14.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.10075093404878345
2	0.0
3	0.03930799627401017
4	0.055812428013052924
5	0.060774314740568984
6	0.0010557205803225648
7	2.1114411606451295E-4
8	5.982416621827868E-4
9	1.0557205803225648E-4
10	0.0
11	2.815254880860173E-4
12	0.0
13	2.1114411606451295E-4
14	0.04909100698499926
15	0.021607081210601828
16	2.1114411606451295E-4
17	1.0557205803225648E-4
18	2.815254880860173E-4
19	3.167161740967695E-4
20	1.0557205803225648E-4
21	0.0030967803689461904
22	2.1114411606451295E-4
23	2.1114411606451295E-4
24	3.167161740967695E-4
25	0.1592730448846643
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2841661.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.2230467066174
#Duplication Level	Percentage of deduplicated	Percentage of total
1	59.22026810800587	20.2669800143869
2	14.760468699522514	10.102964194306466
3	7.717849617093778	7.923849837613489
4	4.720427800116013	6.461896843143424
5	3.038684172970689	5.199651518911748
6	2.1920924006532783	4.501204836766693
7	1.5998160356846263	3.832540523786132
8	1.1087650939836309	3.035625567845507
9	0.8923811610967605	2.748600194068791
>10	4.485197551898376	26.22755940846595
>50	0.19341988298021162	4.486900843485763
>100	0.06762209029421075	3.8731058106546783
>500	0.0016592472828308106	0.3752745462527304
>1k	0.001348138417266941	0.9638458603118106
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	4924	0.17327893791694365	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3124	0.10993570309758977	No Hit
GGTATCAACGCAGAGTACTTTTTTT	3095	0.10891517320327794	No Hit
GGTATCAACGCAGAGTACATGGGGG	2863	0.10075093404878345	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.5190686010752165E-5
2	0.0	0.0	0.0	0.0	3.5190686010752165E-5
3	0.0	0.0	0.0	0.0	3.5190686010752165E-5
4	0.0	0.0	0.0	0.0	3.5190686010752165E-5
5	0.0	0.0	0.0	0.0	3.5190686010752165E-5
6	0.0	0.0	0.0	0.0	3.5190686010752165E-5
7	0.0	0.0	0.0	0.0	3.5190686010752165E-5
8	0.0	0.0	0.0	0.0	3.5190686010752165E-5
9	0.0	0.0	0.0	0.0	3.5190686010752165E-5
10	0.0	0.0	0.0	0.0	3.5190686010752165E-5
11	0.0	0.0	0.0	0.0	3.5190686010752165E-5
12	0.0	0.0	0.0	3.5190686010752165E-5	3.5190686010752165E-5
13	0.0	0.0	0.0	3.5190686010752165E-5	3.5190686010752165E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGTAC	45	6.737201E-4	14.785739	3
GGATCGT	60	2.5874646E-5	14.239614	6
GGTATCA	1785	0.0	12.900809	1
GAATGCG	55	0.003086327	12.082097	7
CGTAAGA	65	7.9908234E-4	11.6986065	2
TAGGGTG	265	0.0	11.113321	5
ACGATTC	60	0.0058880346	11.080714	8
TTGCGCG	60	0.0059081228	11.0756445	18
CTTATAC	220	0.0	10.813272	1
GTATCAA	3690	0.0	10.624553	1
TTCGTTG	145	1.425542E-8	10.491919	19
CGTCGAC	85	6.543771E-4	10.067613	19
CGTAGGG	125	2.554154E-6	9.885323	3
TTACCCT	130	4.2123465E-6	9.505118	4
ACAACCG	130	4.240781E-6	9.500096	5
GTGCTAT	285	0.0	9.348738	1
TATACAC	400	0.0	9.26749	3
TTAGCCT	185	6.6411303E-9	9.248222	4
TCTATAC	185	6.6411303E-9	9.248222	3
TATACTG	290	0.0	9.172507	5
>>END_MODULE