FastQCFastQC Report
Mon 15 Aug 2016
SRR2921192_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921192_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2605063
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT34210.1313212002934286No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA45400.010.7379351
GTATAGA2450.010.4726581
TTAGACA2950.09.6568014
GTTCTAT2401.8189894E-129.5029681
TCAAGAC3550.09.3622893
GTGTAGG4900.09.309031
ACAGCGC1752.6888301E-89.2248978
GTCTTAA3300.09.2149991
TGCACCG1554.2897773E-79.1895365
CATGGGT9450.09.14415554
TAGGTCC1356.888522E-69.14415554
AGACCCG1151.116349E-49.0829915
GTCCTAA2106.6393113E-109.0504461
GTTATAT1804.1998646E-88.9750251
ACGGTCC850.00746190558.9376168
CGTAGAC850.00746308038.9374463
TCTATAC1701.6966624E-78.9374463
GGTATCA21350.08.9020781
CCTATAC1502.7182105E-68.8629673
CTTAGAC2701.8189894E-128.7926263