##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921192_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2605063
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.036642875815286	31.0	28.0	31.0	10.0	34.0
2	26.81518911442833	31.0	27.0	31.0	10.0	34.0
3	26.61962762512845	31.0	26.0	31.0	10.0	34.0
4	30.457011980132535	35.0	30.0	37.0	10.0	37.0
5	30.206188871439963	35.0	30.0	37.0	10.0	37.0
6	30.520054985234523	35.0	31.0	37.0	10.0	37.0
7	30.454379030372777	35.0	31.0	37.0	8.0	37.0
8	30.118468536077632	35.0	30.0	37.0	8.0	37.0
9	31.71885747100934	37.0	31.0	39.0	2.0	39.0
10	31.04342006316162	37.0	30.0	39.0	2.0	39.0
11	31.49900328706062	37.0	31.0	39.0	2.0	39.0
12	31.198759108704856	37.0	30.0	39.0	2.0	39.0
13	31.20007078523629	37.0	30.0	39.0	2.0	39.0
14	32.27455919492158	38.0	31.0	40.0	2.0	41.0
15	31.92518990903483	38.0	31.0	40.0	2.0	41.0
16	31.847481615607762	38.0	31.0	40.0	2.0	41.0
17	32.16356802119565	38.0	31.0	40.0	2.0	41.0
18	32.21679974726139	38.0	31.0	40.0	2.0	41.0
19	31.927131128882486	38.0	31.0	40.0	2.0	41.0
20	31.788658470063872	38.0	31.0	40.0	2.0	41.0
21	31.735475111350475	38.0	31.0	40.0	2.0	41.0
22	30.984905163521958	38.0	29.0	40.0	2.0	41.0
23	31.074020474744756	38.0	30.0	40.0	2.0	41.0
24	30.6981973180687	37.0	30.0	40.0	2.0	41.0
25	30.206009605141986	37.0	28.0	40.0	2.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	215461.0
3	10.0
4	1723.0
5	10371.0
6	12376.0
7	10630.0
8	9102.0
9	8547.0
10	7996.0
11	7666.0
12	7404.0
13	7009.0
14	6937.0
15	7286.0
16	7642.0
17	8447.0
18	10010.0
19	11923.0
20	15096.0
21	18791.0
22	22991.0
23	27191.0
24	31268.0
25	35684.0
26	40503.0
27	44518.0
28	49925.0
29	58708.0
30	69422.0
31	81898.0
32	102846.0
33	128691.0
34	163686.0
35	211158.0
36	276746.0
37	362720.0
38	512681.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.42340286719165	17.549366376988125	12.116693694029264	24.910537061790965
2	15.565564527527362	19.482237339380195	39.87330930565541	25.07888882743703
3	19.150589497277398	23.92288947919454	29.340336632910724	27.58618439061734
4	13.233342380840682	15.633094527768105	35.64769083170139	35.48587225968982
5	13.830401891888261	37.29277239563039	34.14459047798204	14.732235234499319
6	33.587992552926046	34.34195121014952	17.34066524634844	14.729390990575997
7	29.573961198844977	31.882352760526594	19.92645607979197	18.61722996083646
8	26.829009879401895	33.97038636231025	20.53169731011249	18.668906448175367
9	27.499666973396646	15.692866167069178	18.819822818330245	37.987644041203936
10	15.969403090785342	28.361757855851643	32.550483326289104	23.11835572707391
11	35.61697845847427	22.84386069780093	21.902477096940512	19.636683746784282
12	23.751697323095456	25.121456736064484	28.924036321658512	22.202809619181547
13	28.777882922915392	21.477658151190177	25.179884562155813	24.56457436373862
14	22.839593148187323	21.00935123391215	25.88088454525944	30.270171072641084
15	24.99895389016576	27.913742197844133	22.908423353163993	24.178880558826116
16	24.197964249180572	27.340291708009374	24.864989816638047	23.59675422617201
17	23.402098278060247	27.34931107049047	25.583823597179766	23.664767054269518
18	23.240792361434274	27.02870605436504	26.48825019159383	23.242251392606857
19	24.508709712468722	26.319529789554796	26.684150976284073	22.48760952169241
20	25.125650713476443	25.698627949110836	26.154890443635036	23.02083089377768
21	25.409259501009807	25.49614891378635	25.775515845895118	23.319075739308726
22	24.329286878568738	25.062324514360167	26.8892178398212	23.7191707672499
23	24.4436288079071	25.42844593852677	26.039738221623978	24.08818703194215
24	24.064967488230153	25.755187927896316	26.27662012731361	23.903224456559922
25	23.81257978549032	25.29698521726722	26.844486268218088	24.04594872902437
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1689.0
1	1689.0
2	1657.5
3	1626.0
4	1626.0
5	1626.0
6	2063.0
7	2500.0
8	2500.0
9	2500.0
10	3162.0
11	3824.0
12	3824.0
13	3824.0
14	5769.5
15	7715.0
16	7715.0
17	7715.0
18	13563.0
19	19411.0
20	19411.0
21	19411.0
22	31358.5
23	43306.0
24	43306.0
25	43306.0
26	62495.5
27	81685.0
28	81685.0
29	81685.0
30	106184.0
31	130683.0
32	130683.0
33	130683.0
34	157523.0
35	184363.0
36	184363.0
37	184363.0
38	214886.0
39	245409.0
40	245409.0
41	245409.0
42	274021.5
43	302634.0
44	302634.0
45	302634.0
46	329661.0
47	356688.0
48	356688.0
49	356688.0
50	358025.5
51	359363.0
52	359363.0
53	359363.0
54	334629.5
55	309896.0
56	309896.0
57	309896.0
58	272335.5
59	234775.0
60	234775.0
61	234775.0
62	196859.0
63	158943.0
64	158943.0
65	158943.0
66	123987.0
67	89031.0
68	89031.0
69	89031.0
70	65725.5
71	42420.0
72	42420.0
73	42420.0
74	29990.5
75	17561.0
76	17561.0
77	17561.0
78	12499.5
79	7438.0
80	7438.0
81	7438.0
82	5131.5
83	2825.0
84	2825.0
85	2825.0
86	1880.5
87	936.0
88	936.0
89	936.0
90	613.5
91	291.0
92	291.0
93	291.0
94	167.0
95	43.0
96	43.0
97	43.0
98	25.5
99	8.0
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03500875026822768
2	0.053703115817160654
3	0.014817300003877067
4	0.011285715546994448
5	0.00990379119430125
6	4.990282384725436E-4
7	0.003416424094158184
8	0.0018041790160161194
9	0.006372206737418635
10	0.0036083580320322387
11	0.010748300520947095
12	0.008675413991907297
13	0.007600583939812588
14	0.014586979278428199
15	0.006679301038017124
16	0.03128523187347101
17	0.0392696836890317
18	0.022840138607012575
19	0.016966960108066486
20	0.016199224356570265
21	0.023147232907611063
22	0.005719631348646847
23	0.016007290418696207
24	0.012053451298490671
25	0.4672823651481749
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2605063.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.87726659455895
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.59365794930812	40.934960535475234
2	12.54940590549424	13.773541869582798
3	5.159946427096044	8.494912670801744
4	2.6067302476695025	5.722009229658399
5	1.4393765218745505	3.9494524560429354
6	0.908553411506365	2.991535666717849
7	0.5900974557713081	2.2668054777993123
8	0.4221911446456376	1.8534956798864526
9	0.29600482652763005	1.4619542200769637
>10	1.3471285935494375	12.654143520756056
>50	0.05944420258577965	2.209304710875289
>100	0.025439300540716702	2.3816734247535036
>500	0.0010842929092732033	0.42528799724816396
>1k	9.397205213614568E-4	0.880922540325327
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3421	0.1313212002934286	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.67735751496221E-5
2	0.0	0.0	0.0	0.0	7.67735751496221E-5
3	0.0	0.0	0.0	0.0	7.67735751496221E-5
4	0.0	0.0	0.0	0.0	7.67735751496221E-5
5	0.0	0.0	0.0	0.0	7.67735751496221E-5
6	0.0	0.0	0.0	0.0	1.9193393787405525E-4
7	0.0	0.0	0.0	3.838678757481105E-5	1.9193393787405525E-4
8	0.0	0.0	0.0	3.838678757481105E-5	1.9193393787405525E-4
9	0.0	0.0	0.0	3.838678757481105E-5	1.9193393787405525E-4
10	0.0	0.0	0.0	3.838678757481105E-5	2.303207254488663E-4
11	0.0	0.0	0.0	3.838678757481105E-5	2.303207254488663E-4
12	0.0	0.0	0.0	3.838678757481105E-5	9.596696893702763E-4
13	0.0	0.0	0.0	3.838678757481105E-5	0.0012283772023939537
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	4540	0.0	10.737935	1
GTATAGA	245	0.0	10.472658	1
TTAGACA	295	0.0	9.656801	4
GTTCTAT	240	1.8189894E-12	9.502968	1
TCAAGAC	355	0.0	9.362289	3
GTGTAGG	490	0.0	9.30903	1
ACAGCGC	175	2.6888301E-8	9.224897	8
GTCTTAA	330	0.0	9.214999	1
TGCACCG	155	4.2897773E-7	9.189536	5
CATGGGT	945	0.0	9.1441555	4
TAGGTCC	135	6.888522E-6	9.1441555	4
AGACCCG	115	1.116349E-4	9.082991	5
GTCCTAA	210	6.6393113E-10	9.050446	1
GTTATAT	180	4.1998646E-8	8.975025	1
ACGGTCC	85	0.0074619055	8.937616	8
CGTAGAC	85	0.0074630803	8.937446	3
TCTATAC	170	1.6966624E-7	8.937446	3
GGTATCA	2135	0.0	8.902078	1
CCTATAC	150	2.7182105E-6	8.862967	3
CTTAGAC	270	1.8189894E-12	8.792626	3
>>END_MODULE