FastQCFastQC Report
Mon 15 Aug 2016
SRR2921192_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921192_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2605063
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT75210.2887070293501539No Hit
TATCAACGCAGAGTACTTTTTTTTT47570.18260594849337616No Hit
GGTATCAACGCAGAGTACTTTTTTT44290.17001508216883815No Hit
CCCATGTACTCTGCGTTGATACCAC37950.14567785884640794No Hit
ACGCAGAGTACTTTTTTTTTTTTTT30090.11550584381260645No Hit
GTACATGGGAAGCAGTGGTATCAAC28260.10848106168641602No Hit
GGTATCAACGCAGAGTACATGGGGG27620.10602430728162812No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATAC604.0823425E-412.670833
CGATACT604.0823425E-412.670834
TAATACT2550.011.1801434
ACGCAAT700.001497230810.85257915
TTACCCT1355.6648787E-810.5590254
GGTATCA26900.010.50379851
TTATACT2300.010.329484
GTCCTAC1201.497101E-610.3063361
CCCCTAT1409.496398E-810.193081
TTAATAC2900.010.158513
TAGGGTG3100.010.112345
CTATAAG2850.010.0142541
GTATCAA51650.09.8727161
GTACTAA1553.977948E-89.820431
GTCTTAG3200.09.810841
GATCTCG1651.0069925E-89.79730819
TATAAGG2550.09.6894582
ACTATAC1901.0459189E-99.5031223
TCTATAC1304.223124E-69.5031213
CCATGCG800.00451281069.5011149
Produced by FastQC (version 0.11.5)